Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,297	C→T	L303L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,297	0	C	T	97.7%	108.1 / NA	44	L303L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/21);  minor base G (0/1);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGA  >  NC_000913/23053‑23528                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         aCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGAGTtgctgc                                                                                                                                                                                                                                    >  1:110693/1‑251 (MQ=255) aCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTtgctgc                                                                                                                                                                                                                                    <  1:114738/251‑1 (MQ=255)    gAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGTTGCGGAGCTTGAGTTGCTgcg                                                                                                                                                                                                                                   <  2:227347/249‑1 (MQ=255)          aaaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTTCGCTTTAccc                                                                                                                                                                                                                           >  2:8465/1‑251 (MQ=255)          aaaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTAccc                                                                                                                                                                                                                           <  2:157949/251‑1 (MQ=255)                               tAACGGCCCAATCTCGCTGGTGATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGAc                                                                                                                                                                                                       <  1:130852/250‑1 (MQ=255)                                             cGCTGGTGATCTGGACCACGACGCCGTGGACTCTGCCTGCGAACCGCGCAAACTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                                        <  2:41771/251‑1 (MQ=255)                                                   tgaTCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAt                                                                                                                                                                                  >  1:158707/3‑251 (MQ=255)                                                                 acGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTg                                                                                                                                                                      <  1:194089/249‑1 (MQ=255)                                                                      gTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCGGATGTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCg                                                                                                                                                               <  2:13409/251‑1 (MQ=255)                                                                        ggACTCTGCCTGCGAGCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGGCGTTCCGGCAATCCTCGGCGATACCGTTACCCTGGATGCCGGt                                                                                                                                                             >  2:43041/1‑251 (MQ=255)                                                                             ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTGAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCgg                                                                                                                                                        >  2:107463/1‑251 (MQ=255)                                                                                                        gCACCTGATTTCGACTCTGCGCTGGTGACGATAGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTAACCCATTCTCAGCACGGTAAAAGGTGCGGCGCTTGCGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACgg                                                                                                                            >  2:16916/1‑251 (MQ=255)                                                                                                         catctgattgcggcTATGCGCTGGTGCCGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAATGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCTG‑TGCCGTTCACACCTCGCGTGGGCACGGc                                                                                                                           <  2:41774/238‑1 (MQ=255)                                                                                                          acctgATTTCGACTATGCGCTGGTGCAGATCTAGGGGGAGGCCGTGTTTCTGAAGAAAGATCTGGTTGAAAGCGGAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAACGGTGCGGAGCTTAAGTTGCTGCGCCTTACCCATCCGTTTTTGGGCCTCGACAATCCCGCAATCCCCGGCGATCACGTTCACATGGACGCCGGTACCGGGTCCAGTCCACAC‑GCGCCTGGCCCCGGcc                                                                                                                          >  2:232770/5‑251 (MQ=255)                                                                                                              gATTTCGACTATGCGCTGGGGCAGAGCGACGGGCAGGCCGTGACTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAGTCTCCGCACTGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGTCATTCCTCGGCGATCCCGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGga                                                                                                                      <  2:117698/251‑1 (MQ=255)                                                                                                                      cTATGCGCTGGTGCAGCTCGACGGTCAGGCCGTGCTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCCTGAGTTGCTGCGCTTTACCCAACCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGTACCGG‑CTCCGTTACCACCGCGCCTGGTCACGGCCCGGCCGACTAtg                                                                                                              >  2:54037/1‑251 (MQ=255)                                                                                                                        aTGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAACACGGTAAACGGCGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCCATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGGCTAtgtg                                                                                                            >  1:158236/1‑251 (MQ=255)                                                                                                                                    aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGCAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGACCACCGCCCGGACGACTATGTGATCGGCCAGaa                                                                                                 >  1:217128/1‑250 (MQ=255)                                                                                                                                          aCGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCCCGGCCCGGCCGACTATGTGATCGGTCAGAAATACGGc                                                                                          >  2:86159/1‑251 (MQ=255)                                                                                                                                            ggTCAGGCCGTGATTCTGGCGAAAGACCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTTAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑GGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCt                                                                                        <  2:123539/251‑1 (MQ=255)                                                                                                                                             gTCAGGCCGTGATTCGGGGGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGGCCGATTACACCATTCTCATCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCTCGTTACCCATCCGGTTATGGGCTTCGACGTTGCGGCAATCCGCGGCGATCACGTTACCCTGTATGGCTGTGCCTG‑TGCCGTTCACATCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTg                                                                                       <  1:132767/251‑1 (MQ=255)                                                                                                                                                   ccGTTATTCTGGCGCAAGATCTGGGTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCATTCCTCGGCGATCACGTTACCCTGGATGCCGTTACCGG‑TGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAcc                                                                                 <  1:69794/251‑1 (MQ=255)                                                                                                                                                     gTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGCCGTGCCCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGCTGAGCTTTCCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTAACGG‑TGCCGTTCACACCGCGCCTCGCCACGGCACGGCCGACTATGTGCTCGGTCAGAAATACGCCCTGGAAACCGc                                                                               >  1:166124/1‑251 (MQ=255)                                                                                                                                                      tGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAACATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGGACCGG‑TGCCGTTCACACCGCGCCTGGACACGGCCCGGAAGACTATGTGATCGGTCAGAAATGCGGCCTGGAAACCGCt                                                                              >  1:7072/1‑251 (MQ=255)                                                                                                                                                             ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCg                                                                        <  1:8465/250‑1 (MQ=255)                                                                                                                                                               cGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGtt                                                                     <  2:79682/251‑1 (MQ=255)                                                                                                                                                               cGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGAGGAGATTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGGTACCCTGGATGCCGGTACCGG‑GGCCGTTCAGACCGCGCCTGGCCACGGCCCGGACGAATATGTGATCGGTCAGACAAACGGCCTGGAAACCGCTAACCCGGtt                                                                     >  1:152526/1‑251 (MQ=255)                                                                                                                                                                        gggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGAc                                                            <  1:206090/250‑1 (MQ=255)                                                                                                                                                                          gTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACgg                                                          >  1:175060/1‑251 (MQ=255)                                                                                                                                                                             taaaGCGTAATGCAGCGTATCGGCGTGTCCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCa                                                        <  2:219497/249‑1 (MQ=255)                                                                                                                                                                             gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGGGCGGGGCTTGAGTTGCTGCGCTTTACCCACCCGTTTTTGGGCTTCGCCGTTCCGGCAATCCTCGGCAATCCCGTTACCCTGGTTGCCGGGCCCGA‑TGCCGTCAACACCGGGCCTGGCCCCGGCCCGGACGCCTACGTGTTCGGCCAGAACTACGGCCGGAAAACCGCCAACCCGGTTTGCCCGGACGGccc                                                       >  2:234027/1‑249 (MQ=255)                                                                                                                                                                                   gTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCt                                                 <  1:16916/251‑1 (MQ=255)                                                                                                                                                                                     aagtcAGCGTATCGGCGGGACCTATTACACCATTCGCAGCACGGTAAAAGGTGCGGAGCTTTATTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCGGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCTGGAAACCGCTATCCCGGTTGGCCCGTACTGCACTTATCTGc                                               <  2:256872/247‑1 (MQ=255)                                                                                                                                                                                      aTGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGcc                                              <  1:86159/251‑1 (MQ=255)                                                                                                                                                                                         cAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGATTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATAAGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCACTTATCTGCCggg                                           >  1:136360/1‑251 (MQ=255)                                                                                                                                                                                            cGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGGGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCAc                                        >  2:86995/1‑251 (MQ=255)                                                                                                                                                                                             ggagcGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCGGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACt                                       <  2:110693/247‑1 (MQ=255)                                                                                                                                                                                                   gCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                                 >  1:151956/1‑251 (MQ=255)                                                                                                                                                                                                               acacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTGACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCTCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATgg                      <  1:43041/250‑1 (MQ=255)                                                                                                                                                                                                                        ccaccACGGTAAAAGGTGCGGAGCGTGAGGTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTc            <  2:45759/247‑1 (MQ=255)                                                                                                                                                                                                                           gCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTc         >  1:50322/1‑251 (MQ=255)                                                                                                                                                                                                                                gTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGc    >  1:256761/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaGGAGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGACGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGa  >  2:226876/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         ACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGG‑TGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGA  >  NC_000913/23053‑23528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑