| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,038 | C→T | L568L (CTG→TTG) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,038 | 0 | C | T | 91.2% | 90.9 / NA | 34 | L568L (CTG→TTG) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (15/16); minor base G (0/3); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATG > NC_000913/1801‑2218 | aGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCCGCCAGGCAGTGGCGGATCCATAT‑GCCGACTTCTTGCGCGAAGGttt > 2:232900/1‑251 (MQ=255) ggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGGTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATGCGCCTCGTGAAAGAATATCATCTGCTGATCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCAc < 1:23856/251‑1 (MQ=255) ctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGCTCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGt > 1:217794/1‑251 (MQ=255) tgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAAATGGCGCAAGCCAAAGAGCAGTTTAATCTCGGACGCTTAATTCGACTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGGTTCCACggt > 1:159352/1‑249 (MQ=255) aaTAAACATATCTACTTACGTGTCTGCGGTGTTGCCAACTCGAATGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCTTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCAc < 1:33506/251‑1 (MQ=255) aCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGACGTTTAATCTCGGGCGCTTAATTCGCCTCGTGACAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCCATAT‑GCAGACTTCTTGAGCACAGGTTTCCACGTTGTCACGCCg > 2:148335/1‑250 (MQ=255) aCGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGcc < 1:44213/250‑1 (MQ=255) cGTGTCGGCGGTGTTGGCAACGCGAAGGCACTGCTCACCAATGAGCATGGCCTAAATCTAGAAAACTGGCAGTAAGATCTTGCTCAAGCCAAAGATCCGTTTAATCTCGGGTGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCAGGTCATTGTTGGCTGCACTTCCAGCCAGGCATGGGCGGAGCAATAT‑GCCGATTGCGCGCGCGACGGTTACCCCGTGGTCACTCCGAAcgaaccggccaa < 2:110444/251‑12 (MQ=255) tgtCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCCTGGCCTAAATCTGGAAAACTGGCAGGAAGAACTAGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAACGAAGATCATCTGCTGAACCCGGTCATTGTTGAGTGCACTTCCAGCCAGGCAGTGGCGGCCCAATAT‑GCCGGCGTCTTGCGCGCAGGTTTCCACGTTGTCACGCCGCACCAAAAGGCCaaa > 2:184166/1‑249 (MQ=255) actGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATctgt < 1:55153/250‑4 (MQ=255) tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg > 1:171968/1‑251 (MQ=255) tCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCAAACACCTCGTCGATGGATTACTACAATCTGTTGCGt > 1:186638/1‑251 (MQ=255) ccAATGTGCATGGCCTAAATCTGGAAAACTTGCAGGAAGAACTGGCGCAAGTCAAAGAGCCGGTTAAGCTCGGGCGCATAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCTCGTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGAGTTCGTGCGCGAAGGGTTCCGCGTTGTCACGCCTAACAAAAATGCCAACACCGCGGCGAGGGAttgcttccagctgtggtgtcat < 2:139935/251‑21 (MQ=255) gCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACAACTCGTCGATGGATTACTACCATCTGTTTCGTCATGCGGCGGaaaa > 1:161953/1‑250 (MQ=255) aaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATcgcg < 1:1273/251‑1 (MQ=255) ggAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCCTCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCTCGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCgcgg < 1:8281/247‑1 (MQ=255) ggCAGGAAGAACTTGCTCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTTTTGACTTCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCGGACTTCTTGCGCGAAGGTTGCCACGTGGTCTCGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAtt < 1:43917/247‑1 (MQ=255) cgcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGGCTTCTTGCGCGCAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGtt > 1:124544/1‑251 (MQ=255) agCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt > 1:182701/1‑250 (MQ=255) ccGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTAcc < 1:18652/251‑1 (MQ=255) ccGTTTAATCGCGGTGGCTTTATTCGCCTCGTTAAAGAATATCAGCTGCTGAACCGCGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCAGTTCAATAG‑GCCGACTTCGTGCGCGAAGGTGGCCATGTTGTCACGCCGAACAAAAATGTCAACGCCTCGGCGATGGTTTACTTTTATCTGTTTCGTCATGCGGCGGATAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGAGGCTGGATTAcc < 1:239620/251‑1 (MQ=255) ccggCGCTTAATTCGCCTCGTGAAAGAATACCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGGCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGTCTGGATTACCGTTTATTgag < 2:126537/248‑1 (MQ=255) cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt < 1:97620/247‑1 (MQ=255) cGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 2:171968/251‑1 (MQ=255) cTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGa > 1:213701/1‑153 (MQ=255) cTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGa < 2:213701/153‑1 (MQ=255) cTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCCATAT‑GCCGTCTTCTTGCGCGAAGGTTTCCACGTTGTCCCGCCGAACAAAAAGGCCAACACCTCGTCGATGGCTTACTACCATCTGtt > 2:123112/1‑158 (MQ=255) cTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt < 1:123112/158‑1 (MQ=255) gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg > 1:201534/1‑210 (MQ=255) gTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTggg < 2:201534/210‑1 (MQ=255) tGAAAGAATATCATCTGCTGACCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATCGCGGA‑TTCTTGCGCGAAGGTTTCCACGGTGTCACGCAGAACAAACAGGCCAACACCTCGTCGAAGGATTACTACCATATGTTGCGTCCTGCGGCGGAAAAATCGAGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAACAACTGCTCacg > 2:264836/1‑249 (MQ=255) catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatg < 1:176866/250‑1 (MQ=255) aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt > 2:234208/1‑125 (MQ=255) aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt < 1:234208/125‑1 (MQ=255) | AGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATAT‑GCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATG > NC_000913/1801‑2218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |