Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	21,895	C→T	R163R (CGC→CGT)	ribF →	bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	21,895	0	C	T	97.4%	115.4 / NA	39	R163R (CGC→CGT)	ribF	bifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/13);  minor base G (0/1);  total (25/14)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTGCGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAAC  >  NC_000913/21658‑22132                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              gtgtGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCAccg                                                                                                                                                                                                                                  >  2:266664/1‑251 (MQ=255)      gctttGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCTCGCAAAATTTCATCAGCTATCTGCTGGGTAAGCTTTTGGGCGTAAAATTTCTTGCCGTAGGTGAGGATGTCCGCTT‑GGCAGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGAACGCAAACTTTTTACAAAGGTGGCATGCGTAGCAGCATCAccgctttg                                                                                                                                                                                                                             <  2:221181/248‑5 (MQ=255)        gTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCg                                                                                                                                                                                                                           >  1:121963/1‑251 (MQ=255)            aTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTc                                                                                                                                                                                                                         >  2:103397/1‑249 (MQ=255)                          gtgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCgggtg                                                                                                                                                                                                          >  2:136307/1‑247 (MQ=255)                            gcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACa                                                                                                                                                                                                       <  1:103397/251‑1 (MQ=255)                             cgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACa                                                                                                                                                                                                       >  1:184287/1‑250 (MQ=255)                               tttCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtc                                                                                                                                                                                                    >  1:68880/1‑251 (MQ=255)                                  cGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctg                                                                                                                                                                                                  <  1:254307/250‑1 (MQ=255)                                   gACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggc                                                                                                                                                                                                >  1:154343/1‑251 (MQ=255)                                         cGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggctctggc                                                                                                                                                                                          <  1:266664/251‑1 (MQ=255)                                                cggcgTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCaggaagt                                                                                                                                                                                   >  2:194176/1‑246 (MQ=255)                                                     ttAACCGCGAAAAATTTCATCAGCGATATTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAACGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACt                                                                                                                                                                              >  2:180328/1‑251 (MQ=255)                                                                  ctttCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACCATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGACAGTTTACTGGGGCACCCGttt                                                                                                                                                                 >  2:82111/2‑251 (MQ=255)                                                                            cGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGCAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCg                                                                                                                                                       >  1:119776/1‑251 (MQ=255)                                                                                       tGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCTGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTc                                                                                                                                            >  1:225623/1‑251 (MQ=255)                                                                                                            ttCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGgcg                                                                                                                        <  1:23918/250‑1 (MQ=255)                                                                                                                 gCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAACGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGGTGAATTAGGGCGCACtat                                                                                                                  >  2:52232/1‑251 (MQ=255)                                                                                                                    gTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACTGTGATGAATTAGGGCGCACTATAgg                                                                                                               >  1:177842/1‑251 (MQ=255)                                                                                                                    gTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAgg                                                                                                               >  1:29827/1‑251 (MQ=255)                                                                                                                       ggTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGttt                                                                                                            <  1:60191/251‑1 (MQ=255)                                                                                                                        gTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAACGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGCCAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTc                                                                                                           >  1:101570/1‑251 (MQ=255)                                                                                                                                       ttGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGCTGACAATCTGGCTCTGGCAGAAAGTTTCCTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGt                                                                                             >  2:126447/1‑250 (MQ=255)                                                                                                                                                ggTCGTGAAGGCGATTTCTTGTTATGACAGAAAGCTGTCATGGACTACGGCTTCGATATCACAAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGATGCGCGTCAGGCGCTGGCGGATGCGAATCTGGATCAGGCAGAAAGTTTACTAGGGCACCCGGTTGCCATCTCTGGGCGCGTAGTCAAAGGTGATGAATTAGGGCGAACTATAGGATTCTCGACGGCGAATGAACCgacacg                                                                                    >  2:7526/1‑246 (MQ=255)                                                                                                                                                   cGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGAGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCg                                                                                 >  2:149596/1‑251 (MQ=255)                                                                                                                                                   cGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACAAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGTTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCg                                                                                 >  1:40312/1‑251 (MQ=255)                                                                                                                                                                ttttGTTATTACAGAAAGCTTGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGAGAAGGGGGCGTGCGGATCAGCAGCTCCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGTGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTcccc                                                                     <  2:229571/248‑1 (MQ=255)                                                                                                                                                                  ttGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCggg                                                                  >  2:192834/1‑250 (MQ=255)                                                                                                                                                                   tGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCCCCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTTCCGCTACGCCGTCAGGTTTCCCCg                                                                    <  1:149596/248‑1 (MQ=255)                                                                                                                                                                           cAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGt                                                                                                              >  2:63644/1‑198 (MQ=255)                                                                                                                                                                           cAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGt                                                                                                              <  1:63644/198‑1 (MQ=255)                                                                                                                                                                                    ggCATGGAATACGGCTTCGATATTACCAGTACGCAAACTCTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGAACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGt                                                <  1:96279/251‑1 (MQ=255)                                                                                                                                                                                       aTGGAATACGGCTTCGATATCCCCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATTCGGTAGa                                             <  2:40312/251‑1 (MQ=255)                                                                                                                                                                                                    tCGATAGCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACTGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGcc                                  <  2:177842/249‑1 (MQ=255)                                                                                                                                                                                                     cGATATCACCAGTCCGCAAACGTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTc                                <  2:224874/250‑1 (MQ=255)                                                                                                                                                                                                           cACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTAAGGCGCTGGCGGATGACAATCTGGCTCTGGCCGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGACCACGGTGATGAATTAGGGAGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAGCGGGGTTTATGCGGTAGCAGGGCTGGGCCTCGGTGaa                          >  2:170223/1‑250 (MQ=255)                                                                                                                                                                                                                    gCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTAc                >  1:240318/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCGCCGGGCGGGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATTTACCGCGACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCg             <  1:180328/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaGGTGGCGTGCGTATCAGAAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTACGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAAc  >  1:68408/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTCCACGGTGATGAATTAGGGCGCACTATAGGTTTCCCGACGGCGAATGTACCGCTGCGCCGTCAGGTTTCCCCGGTGAAAGGGGTTTATGCGGTAGAAGTGCTGGGCCTCGGTGAAAAGCCGTTACCCGGCGTGGCAAAC  >  NC_000913/21658‑22132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑