Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	21,750	A→T	H115L (CAT→CTT)	ribF →	bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	21,750	0	A	T	100.0%	101.2 / NA	34	H115L (CAT→CTT)	ribF	bifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/11);  total (23/11)

CGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTC  >  NC_000913/21503‑21994                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cgcgcgCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTTCCGGTGATGGTGATTCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGAGTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCtttt                                                                                                                                                                                                                                                    <  2:264254/251‑1 (MQ=255)                     gCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCCCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACAGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCg                                                                                                                                                                                                                                >  1:255403/1‑251 (MQ=255)                                     aagcgcaaCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCCAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCg                                                                                                                                                                                                                >  1:159927/1‑251 (MQ=255)                                            aCTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGgc                                                                                                                                                                                                         >  1:76100/1‑251 (MQ=255)                                                  cGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGt                                                                                                                                                                                                  <  2:76100/251‑1 (MQ=255)                                                   ggtgatggtgatGCTTTTTAAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGTCAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGGATGATTTCCGCTTTGGC‑GCTGGt                                                                                                                                                                                                  >  2:220399/1‑250 (MQ=255)                                                         ggtgatgCTTTTTGAACCTAAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGGTGCGGGAACAACTGCGTTAACTTGCAGAGTGTAGCATTCATTACGTGATGTGCGTGCGTTTCGCCAGGCGCTTCGCGGCGTTCACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGAGTAACATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTggacggga                                                                                                                                                                                             >  1:238369/1‑243 (MQ=255)                                                                  ttttGAACCCCAACCACTGGAACTGTTTGCTACCGATAAAGCGCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTGGCAGAGTGTGGCGTTGATTACGGGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTTCCGTAGGTGATGATTTCCGCTTTGGC‑GCTAGTCGTGAAGGCGAttt                                                                                                                                                                                    <  2:255403/249‑1 (MQ=255)                                                                     tGAACCTCAACCACTGGAACTGTTTGCTACCGTTCAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGt                                                                                                                                                                               >  2:96279/1‑251 (MQ=255)                                                                                     gAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGAACCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGAGTTTCGACCGGCGTTTCGCGGCGTTAACCGCGAAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTATTGTTATTAAAGAAAGCTgg                                                                                                                                                               >  2:194363/1‑251 (MQ=255)                                                                                         tGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAACCTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGTCATg                                                                                                                                                           >  2:148764/1‑251 (MQ=255)                                                                                               cTACCGATAAAGCCACGGCACGGCTGACCCGGCTGCGGGAACAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGGATGCTTTCCGCTTTGGC‑GCTGGTCGTGCAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATAc                                                                                                                                                     >  2:251398/1‑251 (MQ=255)                                                                                                   cGATAACGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCt                                                                                                                                                 <  2:140326/251‑1 (MQ=255)                                                                                                     aTAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTc                                                                                                                                               <  1:65633/251‑1 (MQ=255)                                                                                                         aGCCCCGGCACGGCTGACCCGGCTGCGGGAAACACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGat                                                                                                                                            >  1:224874/1‑250 (MQ=255)                                                                                                          gccccgtcacggcTGACCCGGCTGCGGGAAAAACTGCGTTCCCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGatat                                                                                                                                          <  1:82778/239‑1 (MQ=255)                                                                                                                        gACCCGGCTGCGTGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTGCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCaaa                                                                                                                            <  2:75815/251‑1 (MQ=255)                                                                                                                         aCCCGGCTGCGGGAAAAACTGCGTTCCCTTGCAGAGTGTGTCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCTTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAAc                                                                                                                           <  1:106081/251‑1 (MQ=255)                                                                                                                           ccGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACtt                                                                                                                         >  2:23918/1‑251 (MQ=255)                                                                                                                                                            gtgtGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCAccg                                                                                        >  2:266664/1‑251 (MQ=255)                                                                                                                                                                 gctttGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCTCGCAAAATTTCATCAGCTATCTGCTGGGTAAGCTTTTGGGCGTAAAATTTCTTGCCGTAGGTGAGGATGTCCGCTT‑GGCAGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGAACGCAAACTTTTTACAAAGGTGGCATGCGTAGCAGCATCAccgctttg                                                                                   <  2:221181/248‑5 (MQ=255)                                                                                                                                                                   gTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCg                                                                                 >  1:121963/1‑251 (MQ=255)                                                                                                                                                                       aTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTc                                                                               >  2:103397/1‑249 (MQ=255)                                                                                                                                                                                     gtgcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCgggtg                                                                >  2:136307/1‑247 (MQ=255)                                                                                                                                                                                       gcgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACa                                                             <  1:103397/251‑1 (MQ=255)                                                                                                                                                                                        cgtTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACa                                                             >  1:184287/1‑250 (MQ=255)                                                                                                                                                                                          tttCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtc                                                          >  1:68880/1‑251 (MQ=255)                                                                                                                                                                                             cGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctg                                                        <  1:254307/250‑1 (MQ=255)                                                                                                                                                                                              gACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggc                                                      >  1:154343/1‑251 (MQ=255)                                                                                                                                                                                                    cGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAAtctggctctggc                                                <  1:266664/251‑1 (MQ=255)                                                                                                                                                                                                           cggcgTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCaggaagt                                         >  2:194176/1‑246 (MQ=255)                                                                                                                                                                                                                ttAACCGCGAAAAATTTCATCAGCGATATTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAACGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACt                                    >  2:180328/1‑251 (MQ=255)                                                                                                                                                                                                                             ctttCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACCATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGACAGTTTACTGGGGCACCCGttt                       >  2:82111/2‑251 (MQ=255)                                                                                                                                                                                                                                       cGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGCAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCg             >  1:119776/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCTGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGTATCAGCAGCACCGCTGTGCGTCAGGCGCTGGCGGATGACAATCTGGCTCTGGCAGAAAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTc  >  1:225623/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGATGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGC‑GCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCGATATCACCAGTACGCAAACTTTTTGCGAAGGTGGCGTGCGCATCAGCAGCACCGCCGTGCGTCAGGCCCTTGCGGATGACAATCTGGCTCTGGCAGAGAGTTTACTGGGGCACCCGTTTGCCATCTCCGGGCGTGTAGTC  >  NC_000913/21503‑21994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑