Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,169	T→C	T227T (ACT→ACC)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,169	0	T	C	100.0%	70.5 / NA	26	T227T (ACT→ACC)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

AGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTG  >  NC_000913/17924‑18411                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   aGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTCTCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGc                                                                                                                                                                                                                                               >  1:29836/1‑251 (MQ=255)    cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg                                                                                                                                                                                                                                            >  1:206506/1‑251 (MQ=255)     gtgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggg                                                                                                                                                                                                                                           <  2:10016/251‑1 (MQ=255)                      aagatccattcgatcgccctgccTATTATCATCGCTCTTGGGGCCATCATTATTTTCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGTGGCTGTAGCCATTGCGGTACTAGCGCTATTGCATCTGTGTGGTGTCCGCCGCACGGGCGTCTATATTCTGGCTGGCGTAGTGTTGTGGACTGCGGAGTTGAAAACGGGGGTTCACGCAACCCTGGAGGGGGTAAGTGTCGGcatctt                                                                                                                                                                                                                          >  2:117030/23‑251 (MQ=255)                             ttttGATGGCTCTGGCGATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACCCTAATGACTTAACGATGGCCTCTTTTGGCGGTGCCGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTTGTGTACGCCGCACGGGCGTCTATATTCTGGGTTGCGTAGTGTTGTGGACTGCGGTTTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTa                                                                                                                                                                                                                        <  1:42496/246‑1 (MQ=255)                                         tGGCTATTATCGACGCTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTTTTCCTTTGaaacggacg                                                                                                                                                                                                       >  2:227170/1‑245 (MQ=255)                                                 atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCg                                                                                                                                                                                               <  2:253725/251‑1 (MQ=255)                                                       gtgcTTGGGGCCATCACTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATGGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc                                                                                                                                                                                         <  2:230847/248‑1 (MQ=255)                                                       gaTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTCAAGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTATGTGGTGGACGCCGCACGGGCGTCTATCTTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAACCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGTGCGTTCTcc                                                                                                                                                                                         >  2:106945/1‑251 (MQ=255)                                                            ttgggCCATCATTATCATCGCATTGTTCTACACTAATGTCTTATCGATGGCCTCTCTGGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACGGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGTGGTAATTGTCGGCTTCTTTATTCCTTTTAAAGATAAGCATGGGCGTTCTcc                                                                                                                                                                                         <  2:263834/244‑1 (MQ=255)                                                            tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTtctc                                                                                                                                                                                          <  1:227170/245‑1 (MQ=255)                                                                                     ttCTACACTAATGACTTATCGATGGCCTCTCTTGGTGTCGCGGCTGTATCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACAcc                                                                                                                                                            <  2:4844/250‑1 (MQ=255)                                                                                              aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg                                                                                                                                                  >  2:190855/1‑250 (MQ=255)                                                                                                        cgatgtgcttttttGGCGTCGCGGCTTTATCAAGTGTGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGTTGTTTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGTGGTAATTGTCGGCGTCTTTATTCCGTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCGGGAGCATGTTTTACCCCCGTGGGTGGCGTATCTGAtt                                                                                                                                        <  2:199914/239‑1 (MQ=255)                                                                                                                     tGGCGTCGCGGCTGTAGCAATTGCGGTACGCGCGGTAGTGAATCTGTGTGGTGTACGCCGCGCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGTAATCGGGGTTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCGCCCGTGGGTGGTGTATCTGATTTTGCCGCTGt                                                                                                                              <  1:189824/248‑1 (MQ=255)                                                                                                                              ggCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCTGCTTCTTTATTCCTTTGAAAGAGAAGGATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCt                                                                                                                   >  1:71471/1‑250 (MQ=255)                                                                                                                                              gTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACt                                                                                                  <  1:101696/251‑1 (MQ=255)                                                                                                                                                    gcgGTAATGAATCTGGGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGCGAAGCATGGGCGTTCACCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTATTGCTGGCGGTTCACAGCAAgg                                                                                            >  2:191667/1‑251 (MQ=255)                                                                                                                                                                                    gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGACAGAGAAGCATGGGCGTTCTCCAGCGACGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGCCCTCCATTCTGc                                                            >  2:128704/1‑251 (MQ=255)                                                                                                                                                                                       cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCAt                                                         <  2:77349/251‑1 (MQ=255)                                                                                                                                                                                                          gtagtgtTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCtt                                      <  2:32600/248‑1 (MQ=255)                                                                                                                                                                                                             gtgtTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCTGCTTCTTTATGCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGCTGGCTTGACCTCCATTCTGCCAGTGGGGATCATCGCTGGCTTGCt                                   <  2:231263/251‑1 (MQ=255)                                                                                                                                                                                                                  gtgGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTg                              >  1:207684/1‑251 (MQ=255)                                                                                                                                                                                                                            gggtTGAAATCGGGGGTTCACGCAACCCGGGCGGGGGTAATTGGCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACCCCCGTGGGTGGCGTATCTGATTTTGGCGCGGGTTGGATGTGCTAATGCTGGCGGTTCACTGCAAGGTGTCACGGTGGGTGGCTTGACCTCCATTCTGTCATTGGGGATCATCGCTGGCTCGCTGATTGGTAAACCGCt                    <  2:29836/249‑1 (MQ=255)                                                                                                                                                                                                                                              cACGCAACCCTGGCGGGGGTAATTTTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCATCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGAGTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTctg  <  2:227183/251‑1 (MQ=255)                                                                                                                                                                                                                                              cACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTTTGTTct   <  1:35541/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   AGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTG  >  NC_000913/17924‑18411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑