Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,127	G→A	V213V (GTG→GTA)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,127	0	G	A	100.0%	64.3 / NA	25	V213V (GTG→GTA)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

GACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCAT  >  NC_000913/17885‑18356                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      gACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTtgtg                                                                                                                                                                                                                               <  1:75436/251‑1 (MQ=255)         ctttGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCgg                                                                                                                                                                                                                       >  1:32600/2‑251 (MQ=255)                          tGGCGCAGTTAGGAAGTCGAGTTCCGTTAGCCCTGCAGTTCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGACATCATTATCATCGCCTTGTTCTACACTCATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCCATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTCCGCCGCACGGGCGTATATATTCTGGTTGGCGTAGTGTTGTGGCCTGCGGGGTTGAAATCGGGGGtt                                                                                                                                                                                                      >  2:35541/1‑251 (MQ=255)                           ggCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGtt                                                                                                                                                                                                      <  1:4241/250‑1 (MQ=255)                           ggCGCTGTTGGGAAGTAGTGTTCCGTTAGAGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTATACACTAATGACTTATCGATGGCGTCTATTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGAGTACGTCGCACGGGCGTATATATTATGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTc                                                                                                                                                                                                     >  1:237510/1‑251 (MQ=255)                                        aGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTCTCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGc                                                                                                                                                                                        >  1:29836/1‑251 (MQ=255)                                           cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg                                                                                                                                                                                     >  1:206506/1‑251 (MQ=255)                                            gtgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggg                                                                                                                                                                                    <  2:10016/251‑1 (MQ=255)                                                             aagatccattcgatcgccctgccTATTATCATCGCTCTTGGGGCCATCATTATTTTCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGTGGCTGTAGCCATTGCGGTACTAGCGCTATTGCATCTGTGTGGTGTCCGCCGCACGGGCGTCTATATTCTGGCTGGCGTAGTGTTGTGGACTGCGGAGTTGAAAACGGGGGTTCACGCAACCCTGGAGGGGGTAAGTGTCGGcatctt                                                                                                                                                                   >  2:117030/23‑251 (MQ=255)                                                                    ttttGATGGCTCTGGCGATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACCCTAATGACTTAACGATGGCCTCTTTTGGCGGTGCCGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTTGTGTACGCCGCACGGGCGTCTATATTCTGGGTTGCGTAGTGTTGTGGACTGCGGTTTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTa                                                                                                                                                                 <  1:42496/246‑1 (MQ=255)                                                                                tGGCTATTATCGACGCTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTTTTCCTTTGaaacggacg                                                                                                                                                >  2:227170/1‑245 (MQ=255)                                                                                        atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCg                                                                                                                                        <  2:253725/251‑1 (MQ=255)                                                                                              gtgcTTGGGGCCATCACTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATGGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc                                                                                                                                  <  2:230847/248‑1 (MQ=255)                                                                                              gaTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTCAAGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTATGTGGTGGACGCCGCACGGGCGTCTATCTTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAACCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGTGCGTTCTcc                                                                                                                                  >  2:106945/1‑251 (MQ=255)                                                                                                   ttgggCCATCATTATCATCGCATTGTTCTACACTAATGTCTTATCGATGGCCTCTCTGGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACGGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGTGGTAATTGTCGGCTTCTTTATTCCTTTTAAAGATAAGCATGGGCGTTCTcc                                                                                                                                  <  2:263834/244‑1 (MQ=255)                                                                                                   tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTtctc                                                                                                                                   <  1:227170/245‑1 (MQ=255)                                                                                                                            ttCTACACTAATGACTTATCGATGGCCTCTCTTGGTGTCGCGGCTGTATCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACAcc                                                                                                     <  2:4844/250‑1 (MQ=255)                                                                                                                                     aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg                                                                                           >  2:190855/1‑250 (MQ=255)                                                                                                                                               cgatgtgcttttttGGCGTCGCGGCTTTATCAAGTGTGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGTTGTTTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGTGGTAATTGTCGGCGTCTTTATTCCGTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCGGGAGCATGTTTTACCCCCGTGGGTGGCGTATCTGAtt                                                                                 <  2:199914/239‑1 (MQ=255)                                                                                                                                                            tGGCGTCGCGGCTGTAGCAATTGCGGTACGCGCGGTAGTGAATCTGTGTGGTGTACGCCGCGCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGTAATCGGGGTTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCGCCCGTGGGTGGTGTATCTGATTTTGCCGCTGt                                                                       <  1:189824/248‑1 (MQ=255)                                                                                                                                                                     ggCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCTGCTTCTTTATTCCTTTGAAAGAGAAGGATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCt                                                            >  1:71471/1‑250 (MQ=255)                                                                                                                                                                                     gTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACt                                           <  1:101696/251‑1 (MQ=255)                                                                                                                                                                                           gcgGTAATGAATCTGGGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGCGAAGCATGGGCGTTCACCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTATTGCTGGCGGTTCACAGCAAgg                                     >  2:191667/1‑251 (MQ=255)                                                                                                                                                                                                                           gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGACAGAGAAGCATGGGCGTTCTCCAGCGACGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGCCCTCCATTCTGc     >  2:128704/1‑251 (MQ=255)                                                                                                                                                                                                                              cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCAt  <  2:77349/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      GACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCAT  >  NC_000913/17885‑18356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑