Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,118	T→G	L210L (CTT→CTG)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,118	0	T	G	96.2%	69.9 / ‑5.3	26	L210L (CTT→CTG)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (12/13);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCAT  >  NC_000913/17878‑18356                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ggcTTCTGACCTTGCCTTTTCAATTGGTGTGCTGGCGCTGTTGGGAAGTCTTGTTCCGTTAGCGCTGAAGAGCTTTTTGATGGCTCTGGCTATTATCGCCGATCTGGGGGCCATCATGATCATCGCATTGTTCTACACTAATGACTTATCGATGTCTTTTCTTGGCTTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTGTGGTGTACGCGTCACGGGGGTCTATATTCTTGCTGGcatac                                                                                                                                                                                                                                      <  2:190029/251‑5 (MQ=255)        gACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTtgtg                                                                                                                                                                                                                               <  1:75436/251‑1 (MQ=255)                ctttGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCgg                                                                                                                                                                                                                       >  1:32600/2‑251 (MQ=255)                                 tGGCGCAGTTAGGAAGTCGAGTTCCGTTAGCCCTGCAGTTCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGACATCATTATCATCGCCTTGTTCTACACTCATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCCATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTCCGCCGCACGGGCGTATATATTCTGGTTGGCGTAGTGTTGTGGCCTGCGGGGTTGAAATCGGGGGtt                                                                                                                                                                                                      >  2:35541/1‑251 (MQ=255)                                  ggCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGtt                                                                                                                                                                                                      <  1:4241/250‑1 (MQ=255)                                  ggCGCTGTTGGGAAGTAGTGTTCCGTTAGAGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTATACACTAATGACTTATCGATGGCGTCTATTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGAGTACGTCGCACGGGCGTATATATTATGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTc                                                                                                                                                                                                     >  1:237510/1‑251 (MQ=255)                                               aGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTCTCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGc                                                                                                                                                                                        >  1:29836/1‑251 (MQ=255)                                                  cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg                                                                                                                                                                                     >  1:206506/1‑251 (MQ=255)                                                   gtgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggg                                                                                                                                                                                    <  2:10016/251‑1 (MQ=255)                                                                    aagatccattcgatcgccctgccTATTATCATCGCTCTTGGGGCCATCATTATTTTCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGTGGCTGTAGCCATTGCGGTACTAGCGCTATTGCATCTGTGTGGTGTCCGCCGCACGGGCGTCTATATTCTGGCTGGCGTAGTGTTGTGGACTGCGGAGTTGAAAACGGGGGTTCACGCAACCCTGGAGGGGGTAAGTGTCGGcatctt                                                                                                                                                                   >  2:117030/23‑251 (MQ=255)                                                                           ttttGATGGCTCTGGCGATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACCCTAATGACTTAACGATGGCCTCTTTTGGCGGTGCCGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTTGTGTACGCCGCACGGGCGTCTATATTCTGGGTTGCGTAGTGTTGTGGACTGCGGTTTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTa                                                                                                                                                                 <  1:42496/246‑1 (MQ=255)                                                                                       tGGCTATTATCGACGCTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTTTTCCTTTGaaacggacg                                                                                                                                                >  2:227170/1‑245 (MQ=255)                                                                                               atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCg                                                                                                                                        <  2:253725/251‑1 (MQ=255)                                                                                                     gtgcTTGGGGCCATCACTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATGGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc                                                                                                                                  <  2:230847/248‑1 (MQ=255)                                                                                                     gaTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTCAAGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTATGTGGTGGACGCCGCACGGGCGTCTATCTTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAACCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGTGCGTTCTcc                                                                                                                                  >  2:106945/1‑251 (MQ=255)                                                                                                          ttgggCCATCATTATCATCGCATTGTTCTACACTAATGTCTTATCGATGGCCTCTCTGGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACGGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGTGGTAATTGTCGGCTTCTTTATTCCTTTTAAAGATAAGCATGGGCGTTCTcc                                                                                                                                  <  2:263834/244‑1 (MQ=255)                                                                                                          tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTtctc                                                                                                                                   <  1:227170/245‑1 (MQ=255)                                                                                                                                   ttCTACACTAATGACTTATCGATGGCCTCTCTTGGTGTCGCGGCTGTATCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACAcc                                                                                                     <  2:4844/250‑1 (MQ=255)                                                                                                                                            aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg                                                                                           >  2:190855/1‑250 (MQ=255)                                                                                                                                                      cgatgtgcttttttGGCGTCGCGGCTTTATCAAGTGTGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGTTGTTTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGTGGTAATTGTCGGCGTCTTTATTCCGTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCGGGAGCATGTTTTACCCCCGTGGGTGGCGTATCTGAtt                                                                                 <  2:199914/239‑1 (MQ=255)                                                                                                                                                                   tGGCGTCGCGGCTGTAGCAATTGCGGTACGCGCGGTAGTGAATCTGTGTGGTGTACGCCGCGCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGTAATCGGGGTTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCGCCCGTGGGTGGTGTATCTGATTTTGCCGCTGt                                                                       <  1:189824/248‑1 (MQ=255)                                                                                                                                                                            ggCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCTGCTTCTTTATTCCTTTGAAAGAGAAGGATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCt                                                            >  1:71471/1‑250 (MQ=255)                                                                                                                                                                                            gTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACt                                           <  1:101696/251‑1 (MQ=255)                                                                                                                                                                                                  gcgGTAATGAATCTGGGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGCGAAGCATGGGCGTTCACCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTATTGCTGGCGGTTCACAGCAAgg                                     >  2:191667/1‑251 (MQ=255)                                                                                                                                                                                                                                  gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGACAGAGAAGCATGGGCGTTCTCCAGCGACGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGCCCTCCATTCTGc     >  2:128704/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCAt  <  2:77349/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCAT  >  NC_000913/17878‑18356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑