| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 18,093 | C→T | A202V (GCA→GTA) | nhaA → | Na(+):H(+) antiporter NhaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 18,093 | 0 | C | T | 92.3% | 61.2 / ‑6.4 | 26 | A202V (GCA→GTA) | nhaA | Na(+):H(+) antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/13); minor base G (1/1); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGG > NC_000913/17846‑18321 | ccgatTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGGGTGGTGTAcg > 1:227183/1‑251 (MQ=255) ggTGGGCGATCCCGGCTGGTACTGACATTGCCTTGGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCAGCGCATTGTTCTACACTAATGACTTATCGATGTCCTCTCTTGGCTTCTCGGCTGTTGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCtat < 1:251275/251‑1 (MQ=255) ggcTTCTGACCTTGCCTTTTCAATTGGTGTGCTGGCGCTGTTGGGAAGTCTTGTTCCGTTAGCGCTGAAGAGCTTTTTGATGGCTCTGGCTATTATCGCCGATCTGGGGGCCATCATGATCATCGCATTGTTCTACACTAATGACTTATCGATGTCTTTTCTTGGCTTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTGTGGTGTACGCGTCACGGGGGTCTATATTCTTGCTGGcatac < 2:190029/251‑5 (MQ=255) gACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTtgtg < 1:75436/251‑1 (MQ=255) ctttGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCgg > 1:32600/2‑251 (MQ=255) tGGCGCAGTTAGGAAGTCGAGTTCCGTTAGCCCTGCAGTTCTTTTTGATGGCTCTGGCTGTTATCGACGATCTTGGGGACATCATTATCATCGCCTTGTTCTACACTCATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCCATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTCCGCCGCACGGGCGTATATATTCTGGTTGGCGTAGTGTTGTGGCCTGCGGGGTTGAAATCGGGGGtt > 2:35541/1‑251 (MQ=255) ggCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGtt < 1:4241/250‑1 (MQ=255) ggCGCTGTTGGGAAGTAGTGTTCCGTTAGAGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATAATCGCATTGTTATACACTAATGACTTATCGATGGCGTCTATTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGAGTACGTCGCACGGGCGTATATATTATGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTc > 1:237510/1‑251 (MQ=255) aGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTCTCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGc > 1:29836/1‑251 (MQ=255) cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg > 1:206506/1‑251 (MQ=255) gtgtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggg < 2:10016/251‑1 (MQ=255) aagatccattcgatcgccctgccTATTATCATCGCTCTTGGGGCCATCATTATTTTCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGTGGCTGTAGCCATTGCGGTACTAGCGCTATTGCATCTGTGTGGTGTCCGCCGCACGGGCGTCTATATTCTGGCTGGCGTAGTGTTGTGGACTGCGGAGTTGAAAACGGGGGTTCACGCAACCCTGGAGGGGGTAAGTGTCGGcatctt > 2:117030/23‑251 (MQ=255) ttttGATGGCTCTGGCGATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACCCTAATGACTTAACGATGGCCTCTTTTGGCGGTGCCGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTTGTGTACGCCGCACGGGCGTCTATATTCTGGGTTGCGTAGTGTTGTGGACTGCGGTTTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTa < 1:42496/246‑1 (MQ=255) tGGCTATTATCGACGCTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTTTTCCTTTGaaacggacg > 2:227170/1‑245 (MQ=255) atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCg < 2:253725/251‑1 (MQ=255) gtgcTTGGGGCCATCACTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGGAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATGGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc < 2:230847/248‑1 (MQ=255) gaTCTTGGGGCCATCATTATCATCGCATTGTTCTACACTCAAGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTATGTGGTGGACGCCGCACGGGCGTCTATCTTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAACCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGTGCGTTCTcc > 2:106945/1‑251 (MQ=255) ttgggCCATCATTATCATCGCATTGTTCTACACTAATGTCTTATCGATGGCCTCTCTGGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACGGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGTGGTAATTGTCGGCTTCTTTATTCCTTTTAAAGATAAGCATGGGCGTTCTcc < 2:263834/244‑1 (MQ=255) tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTtctc < 1:227170/245‑1 (MQ=255) ttCTACACTAATGACTTATCGATGGCCTCTCTTGGTGTCGCGGCTGTATCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACAcc < 2:4844/250‑1 (MQ=255) aaTGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtggg > 2:190855/1‑250 (MQ=255) cgatgtgcttttttGGCGTCGCGGCTTTATCAAGTGTGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCCCGGTTGTTTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGTGGTAATTGTCGGCGTCTTTATTCCGTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCGGGAGCATGTTTTACCCCCGTGGGTGGCGTATCTGAtt < 2:199914/239‑1 (MQ=255) tGGCGTCGCGGCTGTAGCAATTGCGGTACGCGCGGTAGTGAATCTGTGTGGTGTACGCCGCGCGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGTAATCGGGGTTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCGCCCGTGGGTGGTGTATCTGATTTTGCCGCTGt < 1:189824/248‑1 (MQ=255) ggCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGGGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCTGCTTCTTTATTCCTTTGAAAGAGAAGGATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCt > 1:71471/1‑250 (MQ=255) gTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACt < 1:101696/251‑1 (MQ=255) gcgGTAATGAATCTGGGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGGTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGCGAAGCATGGGCGTTCACCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTATTGCTGGCGGTTCACAGCAAgg > 2:191667/1‑251 (MQ=255) | CCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGG > NC_000913/17846‑18321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |