Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,830 T→C A114A (GCT→GCC)  nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,8300TC96.2% 81.3 / NA 26A114A (GCT→GCC) nhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (13/12);  minor base A (1/0);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGG  >  NC_000913/17585‑18074
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
gcAACCAGGTGATGGTAACCCGACTTTCTGGAGACGCCGGTTCAGCCCCGGGTTGGTTCCCTCGAAATCAACAAAATCATGCTGTTATGGATAAATGACGCGCTGATGTCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTaa                                                                                                                                                                                                                                                 <  1:200614/251‑1 (MQ=255)
      aGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAGTCGACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGGGAAATGGTGCAAGGATCGCTAACCAGCTTACGACAGGCCGCATTTCCAGTTATCGCCGCTCTTGGTGGGATGCTTGTTTCGGCATTACTCTATCTGGCCTTTAACTATGc                                                                                                                                                                                                                                           >  2:163687/1‑251 (MQ=255)
       gTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGTTTCACTCGAAATCAACAAAAACAGGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGCTCGCTAGCCAGCTTACGCCAGGCCTCATTTCCAGTTAGCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGcc                                                                                                                                                                                                                                          <  2:193165/251‑1 (MQ=255)
                     gACTTTCGGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCcg                                                                                                                                                                                                                            <  1:163687/251‑1 (MQ=255)
                      aCTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCcgc                                                                                                                                                                                                                           <  1:226260/251‑1 (MQ=255)
                             ggAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGt                                                                                                                                                                                                                    >  1:10016/1‑251 (MQ=255)
                                      ggTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATcc                                                                                                                                                                                                           >  1:4844/1‑251 (MQ=255)
                                           aGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCcggcg                                                                                                                                                                                                      <  2:126205/251‑1 (MQ=255)
                                               actggttggttTATTCGAAATCAACAAAATCAGGCTGTTATGGAGAAATGACGCGCTTATGGCGTTATTTTTCCTGTTATTCAGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCTCATTTCCAGTTATCGCCGCTATTGGTGGGAGGATTGTTCCGGCATTACCCTATCTGGCCTTTAACTATCCCGATCCGATTACCCTCGAAGTGTGGGCAATCCCGGCGGCt                                                                                                                                                                                                   <  2:88609/247‑1 (MQ=255)
                                                      ggttCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCTATCCCGGCGGCTACTGACa                                                                                                                                                                                            <  2:172019/250‑1 (MQ=255)
                                                                        aaaaaCATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGTTTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGgtgt                                                                                                                                                                         >  1:262161/1‑251 (MQ=255)
                                                                           aaCATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGgtgtgct                                                                                                                                                                      >  2:59231/1‑248 (MQ=255)
                                                                                   gTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTg                                                                                                                                                               <  1:113017/250‑1 (MQ=255)
                                                                                                       tGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGCAGGTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTAAGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGGCATTTCCTTTGCACTTGGTGTGCTGGCTCTGGTGGGGAGTCGTGTTCCGtt                                                                                                                                           >  2:101696/1‑250 (MQ=255)
                                                                                                                                      ggAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCta                                                                                                           <  1:59231/251‑1 (MQ=255)
                                                                                                                                         aGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGCTGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCAGCGCAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGTACTTTTTGATGGCTCTGGCtattt                                                                                                        >  2:42496/1‑250 (MQ=255)
                                                                                                                                            tAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGACGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGGAGATCTTTTTGATGGTTCTGGCTCTTATcg                                                                                                     >  1:230847/1‑251 (MQ=255)
                                                                                                                                                        gATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTgggg                                                                                         >  1:253725/1‑251 (MQ=255)
                                                                                                                                                            cAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCgtg                                                                                                                                                  >  1:239678/1‑190 (MQ=255)
                                                                                                                                                            cAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACGTGGTGTGCTTGCGCTGTTGGGAAGTCgtg                                                                                                                                                  <  2:239678/190‑1 (MQ=255)
                                                                                                                                                                                                ccAGTTATCGCCGCTATTGGCGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATc                                                 >  1:263834/1‑251 (MQ=255)
                                                                                                                                                                                                     tATCGCCGCAATTGGTGGGATGATTGTTCAGGCATTAATCTATCTGGCATTTCACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATAATTATCATCGCATTGTTCTACACTAATGACTTATCGATgg                                            >  2:191027/1‑251 (MQ=255)
                                                                                                                                                                                                                              gttcccgCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACTATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCTTCTCGTGGCGTCGCGTCTgaag                    <  1:191027/244‑4 (MQ=255)
                                                                                                                                                                                                                                  cGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCCGTAGCAAtt               >  2:4241/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ggCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTAACAGCGCAGGAAGGGCGATCCCGGCGTATACTGAAATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGACGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGTTATTATAGACGGTCTTGGGGGCATCAATATCATCGCATTGTTCTACACTAATGATTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTg              >  1:173854/1‑251 (MQ=255)
                                                                                                                                                                                                                                               gcTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTAACGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGGCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCgg  <  2:227983/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
GCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGG  >  NC_000913/17585‑18074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑