Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,309	A→G	intergenic (+299/‑180)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,309	0	A	G	100.0%	44.2 / NA	16	intergenic (+299/‑180)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/3);  total (13/3)

TCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCT  >  NC_000913/17066‑17554                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         tCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCCTGCATTTTTGTGGCGTAATTTTTTAATGATTTAACTCTTTAACTTTAAATTACCCCTTTATCGCAATTATTGCCGACACGTTGGATTATTTTTGTAATATTGTCCTAACAAATAACGCCGACTGACAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGCAATCCTCACCTTAAGCTATGATTATCTGGGCtt                                                                                                                                                                                                                                                     >  1:240815/1‑249 (MQ=255)      ctccttAACCCATTCGGTTTTATCGATTTTAATTCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATTATTTAATTATTTAACTTGAATTTATCTCTTTATCTCAATTATTGACGACAAGCTGGATTATTTGTGAAATATTGTCCTAACAAATAACTCCGACTGACAAGAAATTAAGTATTTCTTGTCCGAATTAAGCCCGCAGAAATCCTCACCTTAAGCTATCATGATCTGTGCTTAGGGt                                                                                                                                                                                                                                                <  2:246832/245‑1 (MQ=255)                                        cTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCACTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGTGCTTACAGCCGTCa‑aaaaactcatc                                                                                                                                                                                                           >  1:88609/1‑244 (MQ=255)                                                                          ttAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATCATTGACGACAAGCTGAATTATTTTTGAAATATTGTCATTACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCTGAAATCCTCACCTTAAGCTAAGATTATCTGGGATTCGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGTTGGCGAAAATTCTTCattggatcg                                                                                                                                                                         >  1:248674/1‑243 (MQ=255)                                                                                                        tctcTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGATATTCCTACACTATAATc                                                                                                                                           >  1:193165/1‑251 (MQ=255)                                                                                                                   cAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACg                                                                                                                                <  1:182297/251‑1 (MQ=255)                                                                                                                             cgacAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTcc                                                                                                                                                       >  1:15264/1‑218 (MQ=255)                                                                                                                                     tGGATTATTTTTGGAATATTGTCCTAACAAATAACGACGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCACAAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTACTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTa                                                                                                               >  2:96204/1‑250 (MQ=255)                                                                                                                                                            ctaacaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCAc                                                                                      >  2:226260/12‑251 (MQ=255)                                                                                                                                                                         ccGACTGACAAGACATTAATTATTAATTTTACTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATATGGACTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGG‑TACAAATAGTACAAACGATCTATTCACCTGAAAGCGAAAt                                                                         >  1:190029/1‑251 (MQ=255)                                                                                                                                                                                                                 cccTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGc                                 >  1:9626/1‑251 (MQ=255)                                                                                                                                                                                                                 cccTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGACGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGc                                 >  2:113017/1‑251 (MQ=255)                                                                                                                                                                                                                     cagaaaTCCTCACCTTAAGCTATGATTATCTGGGCTTATGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCACCGATTCTTTACCAGTGATGCCTc                              <  2:24472/246‑1 (MQ=255)                                                                                                                                                                                                                      agaaaTCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCgg                            >  2:186550/4‑251 (MQ=255)                                                                                                                                                                                                                                        cTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATc           >  2:74961/1‑250 (MQ=255)                                                                                                                                                                                                                                                aTCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCt  >  2:75436/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         TCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCT  >  NC_000913/17066‑17554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑