Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,797	G→A	Q487Q (CAG→CAA)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,797	0	G	A	100.0%	75.8 / NA	24	Q487Q (CAG→CAA)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

ACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCA  >  NC_000913/1558‑2009                                                                                                                                                                                                                                                |                                                                                                                                                                                                                      aCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGAGCGCGTTACTCATCAGATGCTGATCAATACCGATCAGGGTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGGTGCTGGAGCAACTGAAGCGTCCACAAAgatggcg                                                                                                                                                                                                            >  2:1273/1‑250 (MQ=255)     tGCGTGGGATCTCGGCGAAATGCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCTTCAACAAAGCTGGCTgaa                                                                                                                                                                                                         <  1:103020/250‑1 (MQ=255)     tGCGTGGGATCTCGGCGAAATGCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTgaa                                                                                                                                                                                                         <  2:135329/250‑1 (MQ=255)         ggggATCTCGGCGAAATTCTTTGCCGCGCTGGCACGCGCCAATATCAACATTGGCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGGCCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTTGGTGGCGGTGGCGGAGCGCTGCTGGAGCAACTGAAGCCTCAACAAATCGGGCTGAAGAAT‑a                                                                                                                                                                                                   <  2:175886/250‑1 (MQ=255)                 cGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCGGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGGGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAAc                                                                                                                                                                                                <  2:133814/246‑1 (MQ=255)                                                 aaTATCAACATTGGCGCCATTGCTCAGGGATCTTCTGAACGCTCAAGCTCTGTCGTGGTAAAGAACGATGATGCGACCGCTGGCGTGCGCGTTACTCATCAGATGCTGGTCAATACCGATCAGGTGATCGAAGTGTTTGTTATTGGCGTCGGGGGCGTTGGCGGTGCGCTGCTGGATGAACGGAAGCGTCAACAAAGCTGGCTGA‑GTATGAAAGATATCGACTTCCGTGGCTGCGGTGTTGcgagttcgaa                                                                                                                                                           <  2:254309/251‑10 (MQ=255)                                                               cGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCAcc                                                                                                                                              >  1:173823/1‑250 (MQ=255)                                                                 ccATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAAt                                                                                                                                           >  2:55153/1‑251 (MQ=255)                                                                     tGCTCAGGGATCTTCGGAACGCGCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGGGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGGGTCGGCGGTGTTGCCAACTCGAAGGCGGTGCTCACCAATggg                                                                                                                                        <  2:109980/250‑3 (MQ=255)                                                                              aTCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCta                                                                                                                               >  1:126537/1‑249 (MQ=255)                                                                                  tctGAACGCTCCATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGGGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCt                                                                                                                          >  2:176866/1‑251 (MQ=255)                                                                                  tctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGCCCACTGGCGTGCGCGGTACTCATCAGATGTTGTTCAATACCGGTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCATCTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATc                                                                                                                           <  1:142238/250‑1 (MQ=255)                                                                                  tctGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACGCGAAGGCACTGCTCACCAATGTGCATGGCCTAAAt                                                                                                                            <  2:173823/249‑1 (MQ=255)                                                                                                         ggTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGGGTTTGGGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAAAATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTCAATCTCGAAAACTGGCAGGAAGAACTgg                                                                                                   >  1:451/1‑251 (MQ=255)                                                                                                                   gatgatGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGGTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCa                                                                                          >  2:97620/1‑250 (MQ=255)                                                                                                                      gatgCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAaga                                                                                      <  1:128914/251‑1 (MQ=255)                                                                                                                                                    caGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGCGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AACCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGACCTGGCGCAAGCCCAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCt                                                        >  2:23856/1‑251 (MQ=255)                                                                                                                                                          cTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGa                                                   >  2:44213/1‑250 (MQ=255)                                                                                                                                                            gTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAg                                                <  1:260880/251‑1 (MQ=255)                                                                                                                                                                               aTCGAAGTGTGTGTGATTGGCGTCGGTGGCGTTGGCGGCGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAcc                              <  1:264113/250‑1 (MQ=255)                                                                                                                                                                                                            agctGGCGGGGCGCTGCTGGAGCAACTGAAGCTTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACCTACGTGTCTGCGGTGTTGCCAACTCGAATGCACTGCTCACCAATGAGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCGGCTGAACCCGTGCAATGTTGTCTGCACTAccagcca  <  2:15043/246‑1 (MQ=255)                                                                                                                                                                                                                ggCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGCCT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCAAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTccagcca  >  1:15043/1‑245 (MQ=255)                                                                                                                                                                                                                                         aGCGTCAACCAAGCTGGCTGACGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGcc                                                                                   >  1:50413/1‑139 (MQ=255)                                                                                                                                                                                                                                         aGCGTCAACAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAAGGTGCATGGCCTAAAGCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGcc                                                                                   <  2:50413/139‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                      ACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAAT‑AAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCA  >  NC_000913/1558‑2009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑