Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,245	C→G	intergenic (+235/‑244)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,245	0	C	G	100.0%	31.3 / NA	11	intergenic (+235/‑244)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/2);  total (9/2)

ACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAAT  >  NC_000913/17034‑17483                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  acagatagCCTCAAGCACCCGCCGCTCTTATATCGCTCTCTTTAAACAATTCTGTTTTATCGCTTCTCATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTCTTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAAt                                                                                                                                                                                                            >  2:116379/1‑251 (MQ=255)                                 tCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCCTGCATTTTTGTGGCGTAATTTTTTAATGATTTAACTCTTTAACTTTAAATTACCCCTTTATCGCAATTATTGCCGACACGTTGGATTATTTTTGTAATATTGTCCTAACAAATAACGCCGACTGACAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGCAATCCTCACCTTAAGCTATGATTATCTGGGCtt                                                                                                                                                                              >  1:240815/1‑249 (MQ=255)                                      ctccttAACCCATTCGGTTTTATCGATTTTAATTCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATTATTTAATTATTTAACTTGAATTTATCTCTTTATCTCAATTATTGACGACAAGCTGGATTATTTGTGAAATATTGTCCTAACAAATAACTCCGACTGACAAGAAATTAAGTATTTCTTGTCCGAATTAAGCCCGCAGAAATCCTCACCTTAAGCTATCATGATCTGTGCTTAGGGt                                                                                                                                                                         <  2:246832/245‑1 (MQ=255)                                                                        cTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCACTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGTGCTTACAGCCGTCa‑aaaaactcatc                                                                                                                                    >  1:88609/1‑244 (MQ=255)                                                                                                          ttAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATCATTGACGACAAGCTGAATTATTTTTGAAATATTGTCATTACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCTGAAATCCTCACCTTAAGCTAAGATTATCTGGGATTCGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGTTGGCGAAAATTCTTCattggatcg                                                                                                  >  1:248674/1‑243 (MQ=255)                                                                                                                                        tctcTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGATATTCCTACACTATAATc                                                                    >  1:193165/1‑251 (MQ=255)                                                                                                                                                   cAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACg                                                         <  1:182297/251‑1 (MQ=255)                                                                                                                                                             cgacAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTcc                                                                                >  1:15264/1‑218 (MQ=255)                                                                                                                                                                     tGGATTATTTTTGGAATATTGTCCTAACAAATAACGACGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCACAAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTACTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTa                                        >  2:96204/1‑250 (MQ=255)                                                                                                                                                                                            ctaacaaataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCAc               >  2:226260/12‑251 (MQ=255)                                                                                                                                                                                                         ccGACTGACAAGACATTAATTATTAATTTTACTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATATGGACTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGG‑TACAAATAGTACAAACGATCTATTCACCTGAAAGCGAAAt  >  1:190029/1‑251 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA‑AAATAGTAAAAACGATCTATTCACCTGAAAGAGAAAT  >  NC_000913/17034‑17483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑