Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,175	C→T	intergenic (+165/‑314)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,175	0	C	T	100.0%	18.1 / NA	7	intergenic (+165/‑314)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

TCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATC  >  NC_000913/16994‑17418                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGCTAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTACCTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTAaca                                                                                                                                                                                  >  1:24472/1‑250 (MQ=255)                                         acagatagCCTCAAGCACCCGCCGCTCTTATATCGCTCTCTTTAAACAATTCTGTTTTATCGCTTCTCATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTCTTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAAt                                                                                                                                         >  2:116379/1‑251 (MQ=255)                                                                         tCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCCTGCATTTTTGTGGCGTAATTTTTTAATGATTTAACTCTTTAACTTTAAATTACCCCTTTATCGCAATTATTGCCGACACGTTGGATTATTTTTGTAATATTGTCCTAACAAATAACGCCGACTGACAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGCAATCCTCACCTTAAGCTATGATTATCTGGGCtt                                                                                                           >  1:240815/1‑249 (MQ=255)                                                                              ctccttAACCCATTCGGTTTTATCGATTTTAATTCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATTATTTAATTATTTAACTTGAATTTATCTCTTTATCTCAATTATTGACGACAAGCTGGATTATTTGTGAAATATTGTCCTAACAAATAACTCCGACTGACAAGAAATTAAGTATTTCTTGTCCGAATTAAGCCCGCAGAAATCCTCACCTTAAGCTATCATGATCTGTGCTTAGGGt                                                                                                      <  2:246832/245‑1 (MQ=255)                                                                                                                cTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCACTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGTGCTTACAGCCGTCaaaaaactcatc                                                                  >  1:88609/1‑244 (MQ=255)                                                                                                                                                  ttAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATCATTGACGACAAGCTGAATTATTTTTGAAATATTGTCATTACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCTGAAATCCTCACCTTAAGCTAAGATTATCTGGGATTCGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGTTGGCGAAAATTCTTCattggatcg                                >  1:248674/1‑243 (MQ=255)                                                                                                                                                                                tctcTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGATATTCCTACACTATAATc  >  1:193165/1‑251 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATC  >  NC_000913/16994‑17418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑