Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,085	T→C	intergenic (+75/‑404)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,085	0	T	C	100.0%	19.6 / NA	9	intergenic (+75/‑404)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/4);  total (5/4)

GCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGT  >  NC_000913/16842‑17319                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGGTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAACACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGtttt                                                                                                                                                                                                                                      >  1:206985/1‑250 (MQ=255)                                 ggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                                                                                                                                                                                                     >  2:182297/1‑251 (MQ=255)                                        agcctcgccttttttggctTCTTTTCTCTTTTCTCTTTGCCCTTACGGTCAGTAATAGGCACTCTAAATGTGTTAAGCTTATAGGGGGCCTGGGGTTGATAGTAAAATATCACTCGGGGTTTTTTT‑CTCCCGGCCTTCCAGCAAAGCCCTGAGCAAGATCCCCGAAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCg                                                                                                                                                                                              <  2:189037/235‑1 (MQ=255)                                                          ttaattcctctctTCTCCTTTCCCTTACGGTGAGTAAGAGGCTCTCTACATGTTTCCAGAATAGAGGGGCCCTGGGGTTGATAGAAAAATCTCCCTCGGGCTTTTTCT‑CTAGCTCCCTGCCAGCTAATGCCGGAGAAAAATAGCCTAACGTTCCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt                                                                                                                                                                               <  1:197221/241‑1 (MQ=255)                                                                                     cGGTCAGTAAGAGGCACTTTACGTGTTTTCAGCATAGAGGGTGCCTCGTGTTGATATTAAATTACCACTCGGGGTTTTTCTTCT‑TCTGCGGTCCAGCTAATGCCTGAGACAAATAGCCCCAGGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCttt                                                                                                                                                  <  1:234209/250‑2 (MQ=255)                                                                                                                                                         tCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGCTAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTACCTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTAaca                                                                              >  1:24472/1‑250 (MQ=255)                                                                                                                                                                                                  acagatagCCTCAAGCACCCGCCGCTCTTATATCGCTCTCTTTAAACAATTCTGTTTTATCGCTTCTCATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTCTTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAAt                                     >  2:116379/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCCTGCATTTTTGTGGCGTAATTTTTTAATGATTTAACTCTTTAACTTTAAATTACCCCTTTATCGCAATTATTGCCGACACGTTGGATTATTTTTGTAATATTGTCCTAACAAATAACGCCGACTGACAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGCAATCCTCACCTTAAGCTATGATTATCTGGGCtt       >  1:240815/1‑249 (MQ=255)                                                                                                                                                                                                                                       ctccttAACCCATTCGGTTTTATCGATTTTAATTCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATTATTTAATTATTTAACTTGAATTTATCTCTTTATCTCAATTATTGACGACAAGCTGGATTATTTGTGAAATATTGTCCTAACAAATAACTCCGACTGACAAGAAATTAAGTATTTCTTGTCCGAATTAAGCCCGCAGAAATCCTCACCTTAAGCTATCATGATCTGTGCTTAGGGt  <  2:246832/245‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           GCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGT  >  NC_000913/16842‑17319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑