Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	16,981	G→A	noncoding (30/59 nt)	sokC →	small regulatory RNA antitoxin SokC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	16,981	0	G	A	100.0%	30.2 / NA	13	noncoding (30/59 nt)	sokC	small regulatory RNA antitoxin SokC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

CGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTC  >  NC_000913/16743‑17175                                                                                                                                                                                                                                               |                                                                                                                                                                                                    cgTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCAc                                                                                                                                                                                         >  2:217410/1‑251 (MQ=255)    tgcactTACTCGGATTCGTAAGCCGTGAAAACAGCACCCTCGGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTTTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTc                                                                                                                                                                                       <  1:199857/246‑1 (MQ=255)                    cGTAATCCGTAAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATc                                                                                                                                                                      <  1:184349/250‑1 (MQ=255)                    cGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTGCGGATGTGACCCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCt                                                                                                                                                                     <  1:257887/251‑1 (MQ=255)                                       aaCCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCCGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGGCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCtt                                                                                                                                                  >  2:134344/1‑250 (MQ=255)                                                     cAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGGAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGagaccg                                                                                                                                            >  2:9763/1‑241 (MQ=255)                                                     cAGTCCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGagacag                                                                                                                                            <  1:9763/243‑1 (MQ=255)                                                                                       cgtcacccgcgcCGCCACTACGGGGGTGATAAAGAGGACGATCGGGGCGACAATCATCGCCTTATGCTTCTTCATTGCTCTCTTCCCCTGGACCTTGCGGTCAGAAAGAGGCCCTCTACATGTGTCCAGCATATGGGGGGCCTGGGGTTAATAGTAAAATATCCCGCGGGGTTTTTCT‑CTATCGGCCGTTCAGCTAATGCCGGAGACAGATAGCCAAAAGCACCCGCCGCTATTATATCGCTCTCttt                                                                                                     <  1:217410/240‑1 (MQ=255)                                                                                                    gccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGGTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAACACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGtttt                                                                                      >  1:206985/1‑250 (MQ=255)                                                                                                                                    ggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                                                     >  2:182297/1‑251 (MQ=255)                                                                                                                                           agcctcgccttttttggctTCTTTTCTCTTTTCTCTTTGCCCTTACGGTCAGTAATAGGCACTCTAAATGTGTTAAGCTTATAGGGGGCCTGGGGTTGATAGTAAAATATCACTCGGGGTTTTTTT‑CTCCCGGCCTTCCAGCAAAGCCCTGAGCAAGATCCCCGAAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCg                                              <  2:189037/235‑1 (MQ=255)                                                                                                                                                             ttaattcctctctTCTCCTTTCCCTTACGGTGAGTAAGAGGCTCTCTACATGTTTCCAGAATAGAGGGGCCCTGGGGTTGATAGAAAAATCTCCCTCGGGCTTTTTCT‑CTAGCTCCCTGCCAGCTAATGCCGGAGAAAAATAGCCTAACGTTCCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt                               <  1:197221/241‑1 (MQ=255)                                                                                                                                                                                        cGGTCAGTAAGAGGCACTTTACGTGTTTTCAGCATAGAGGGTGCCTCGTGTTGATATTAAATTACCACTCGGGGTTTTTCTTCT‑TCTGCGGTCCAGCTAATGCCTGAGACAAATAGCCCCAGGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCttt  <  1:234209/250‑2 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                    CGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCT‑CTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTC  >  NC_000913/16743‑17175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑