| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 16,749 | T→A | intergenic (+192/+2) | insL1 → / ← hokC | IS186/IS421 family transposase/protein HokC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 16,749 | 0 | T | A | 88.9% | 38.7 / ‑5.6 | 18 | intergenic (+192/+2) | insL1/hokC | IS186/IS421 family transposase/protein HokC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (13/3); minor base C (1/1); total (14/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCAC > NC_000913/16514‑16993 | tGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCATGACAGCCTACAAAACGCAATTGCAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCgg > 1:167338/1‑250 (MQ=255) ggATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGACATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGGCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTACCCGCCGTTGCACTTACTCGGAttagt > 1:246832/1‑247 (MQ=255) cccccccAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTAAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGt > 1:189037/1‑251 (MQ=255) gtgGAGAAAAACAAAAATGTGCATCTGGAGTTTACAGGTGCCCATTCGTGGGACATTATCCCTGACAGCCTACAAAACGCAATGGAAGACCGCGAGCCATCTTCTCAACGAGCCCCCGAGGCGTCGCATTCTTCAGATGTTTCACCCCTTAAGTGAGCGCTTATGGGATCACCCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTca < 1:37264/251‑1 (MQ=255) cacgggTGCCCAGTCGGGGGACAGTCCCCCTGACAGCCAAAAAACCAAAAATGAAGACCGCGAGGCATCCTATTAAGGCGGCACCGGGGCGTCCCTTCCTCCAGATGTTCCCCCCCTAAATTTGGCGCTTAGGGGACCCCTCCCCGCCGTTGCACTTACTCGGATTCGGAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGACCCTCACAGAGGTCTTTTCGCGTTACCAGCGCCGCCAc < 2:123013/247‑1 (MQ=255) ggCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGCAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTAcggcg > 1:186782/1‑251 (MQ=255) tATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCACCCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGGTGACggt > 1:90531/1‑249 (MQ=255) aaaCGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCTCCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGCAACAGATGATGCTCAGGGCGACAATCATCGc > 2:203684/1‑251 (MQ=255) aTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTAtgc > 1:96490/1‑251 (MQ=255) cGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGACCCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATAACGATCAGAGCGCCAATCAACGCCTTATGCTGCTTTATTGCTCTCTTCTCCTTGACCTTACTGTc > 2:197221/1‑251 (MQ=255) cGAGGCCTCGAATTCTTCCGATGGTTCAACCCTTAAGATAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCCGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGACTACTAACAGGGAGTCACTCATCCCCTTAATCTGCCTCATTGCTCTCTTCTCCTTGACCTTACgggc > 2:39901/1‑249 (MQ=255) aGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTACGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGCCAATCATCGCCTTTTGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTc > 2:257887/1‑249 (MQ=255) aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCCTCGCCCTCTGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACCGTCAgggatcgga > 2:171444/1‑242 (MQ=255) cAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAggg > 2:82583/1‑170 (MQ=255) cTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCCCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAAACATCGCCTTTTGCTGCTTAATTTCTCTCTTTTCCTTGACCTTACGGTTAGTAAGAGGCCCTCTCCAtttttttagcatt > 2:46769/1‑239 (MQ=255) agggatccccTCCCCGCCGTTGCACTTCCTCGGATTGGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGATGTTAACCTCACAGAGGTCTTTTCTCGTTCCCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCggg < 1:86656/241‑1 (MQ=255) ccccccccccccCGTGGCCCTTACTCGGTTTCGTAAGCCGTGAAAACAGCAACCTCCGTCGGGCCAGTTCGGATGTGAACCCCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGAta < 2:96490/245‑2 (MQ=255) cgTTGCACTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCAc > 2:217410/1‑251 (MQ=255) | TGGATTTCCCCCCCAGAAGTGCCGGATCCGAAAAGAAGAACTAACTCGTTGTGGAGAATAACAAAAATGGTCATCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCAC > NC_000913/16514‑16993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |