Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,701	T→C	A178A (GCT→GCC)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,701	0	T	C	97.1%	107.5 / ‑4.8	35	A178A (GCT→GCC)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/16);  minor base A (1/0);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

GGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTC  >  NC_000913/14462‑14935                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           ggCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCTCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGAc                                                                                                                                                                                                                                 <  1:102667/251‑1 (MQ=255)            tGGCTGCGGACGTGGGCGTCAACGTTTGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCGCCCCCTCGAAGAAGCTGTACGTGGCGCTACCAAATAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTCCACTGCCGCAGTCCTGACCGTCCTGTCATGGTGCTGGTCAGGTGCAGATGCTTCAGGGATTCTTTGCCGGGCAGCAGACCTGCCCacac                                                                                                                                                                                                                       <  2:197587/250‑1 (MQ=255)             ggcggcGTATGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCTCCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTTGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGGCCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTg                                                                                                                                                                                                                     <  2:190479/251‑1 (MQ=255)               cggcggACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTc                                                                                                                                                                                                                   >  2:52104/1‑251 (MQ=255)                ggcggACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCAGTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGGCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCa                                                                                                                                                                                                                  <  2:62718/251‑1 (MQ=255)                    gACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGc                                                                                                                                                                                                              >  2:114510/1‑251 (MQ=255)                               cAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGCAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGACGAGTGTGACGTTTGCCACGGTAGAGGTGCAACACCAGGTGCACAGCCGCAGACCTGTCCGACCTGTCATGGATCTGGTCAGGTGCAGATGCGTCAGGGCTTCTTTGCCGTGCCGCCGACCTGTCCACACTGTCAGGGCCGCGGTACGcg                                                                                                                                                                                                   >  1:119046/1‑250 (MQ=255)                                              ggtgcgtgtttACGCTATAACATGGAGCTCTCCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGACCCGCATTCCGACTCTCGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTg                                                                                                                                                                                    <  1:88666/243‑1 (MQ=255)                                                     aTTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGcaacaa                                                                                                                                                                              >  2:233473/1‑250 (MQ=255)                                                          cgcgataACATGTAGCCCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGCCGTTTGCCACGGTAGCGGTGCAAATCCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAGTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtca                                                                                                                                                                        <  2:58043/247‑1 (MQ=255)                                                                   ctGGAGCTCACCCTCGCACCAGCTGTACGTGGCGTGACCAAAGAGATCCGCCTTCCGACTCTGGAAGCGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGGGCAGCTGCGTCAGGGCTTCTTTGCCGGGCAGCAGACCTTTCCACACTGTCAGGGCCGCGGTACGCTGGTCAACGATCCGTGCACCAAATgtcatgtgcatgg                                                                                                                                                               >  2:12521/2‑244 (MQ=255)                                                                      gAGCTCACCCTCGAAGAAGCTGTACGTGGAGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCg                                                                                                                                                            >  1:76485/1‑251 (MQ=255)                                                                                  gcagaagCTGTACGTGGCGAGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGGACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTGGAgcgc                                                                                                                                                  >  2:3890/3‑249 (MQ=255)                                                                                    agaagCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCgcagc                                                                                                                                               >  1:245582/1‑250 (MQ=255)                                                                                             aCGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCAGTGCAAAACCATGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGTTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTg                                                                                                                                      <  1:151062/250‑1 (MQ=255)                                                                                                       aCCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAACCGCTGTCCGTTAAAAt                                                                                                                           >  1:16560/1‑251 (MQ=255)                                                                                                       aCCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGAGTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGGTGAGCGCAGCGAAACGCTGTCCGTTAAAAt                                                                                                                           <  2:232220/251‑1 (MQ=255)                                                                                                             gagaTCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATgtcat                                                                                                                                                                       >  1:17241/1‑201 (MQ=255)                                                                                                             gagaTCCGCATTCCGACTCTGGAAGAGTGGGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCATCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAAGgtcat                                                                                                                                                                       <  2:17241/201‑1 (MQ=255)                                                                                                                    gCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTg                                                                                                              <  1:52104/251‑1 (MQ=255)                                                                                                                          cGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGTTACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGGCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAAGCCCGGCAGGTGTAGACACt                                                                                                        <  2:123256/251‑1 (MQ=255)                                                                                                                                 ggAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAACACCAGGAACACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACCCTggcaacc                                                                                                 >  2:229179/1‑246 (MQ=255)                                                                                                                                      agTGTGACGTTTGCCACGGTAGCGGTGCAAAACCCGGTACACAGCCGCAGACCTGTCCGACCTGGCATGGTTCTGATCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACACATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATACCGGCAGGGGTGGACACTGGAGACCGCATc                                                                                            >  2:236726/1‑251 (MQ=255)                                                                                                                                                                       cAGGTACACAGCCGCAGACCTGTCCTACCTGTCTTGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCATCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCAt                                                           <  1:178294/251‑1 (MQ=255)                                                                                                                                                                        aGGTGCACAGCCGCAGACCTGTCCGACCTGTCATGGTTCTGGACAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGGACGCTGAGCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCGTGCGGGCGAAGGTGAAGCGGGCGTGCATg                                                          <  2:127553/251‑1 (MQ=255)                                                                                                                                                                                 gCCGCAGACCTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCgg                                                 >  1:213111/1‑251 (MQ=255)                                                                                                                                                                                    gCAGACCTGTCCGACCTGCCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTAAGCTGATCAAAGAGCCGTGCAACAAATGTCATGGTCATGGTCGTGTTAAGCGCAGCAAAACGCTGTCCGGTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGCCTAGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGc                                                >  2:202968/1‑249 (MQ=255)                                                                                                                                                                                              ccGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGt                                     >  1:113923/1‑250 (MQ=255)                                                                                                                                                                                                gACCTGTCATGGTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCAGTGCAGCAGACCTGTCCACACTGTCCGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAACTGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTACGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGGGAAGCGGGCGCGCATGGCTCACCGGCAGGCGATATGTACg                                  >  2:185297/1‑251 (MQ=255)                                                                                                                                                                                                           gTTCTGGTCAGGTGCAGATGCGTCAGGGATTCTATGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCCAAGATCCGTGCAACACATGTCGTGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGGGGACACTGGAGACCACATCCGTCTTGCGTGCGAAGGGGAAGCGGGCGAGCATGGCGCACCGGCAGGAGATCTGTCCgttcaggttccg                       >  2:151857/1‑249 (MQ=255)                                                                                                                                                                                                              cTGGTCAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGac                                                                                                           <  1:36022/164‑1 (MQ=255)                                                                                                                                                                                                              cTGGTCAGGAGCCGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGAGGTACGCTGATCAAAGCTCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGac                                                                                                           >  2:36022/1‑164 (MQ=255)                                                                                                                                                                                                                   cAGGTGCAGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAAc                <  2:235832/250‑1 (MQ=255)                                                                                                                                                                                                                          aGATGCGTCAGGGATTCTTTGCCGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCg        <  1:39959/251‑1 (MQ=255)                                                                                                                                                                                                                                gtcAGGGATTCTTTGACGTGCAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTc  >  1:71884/3‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GGCGATATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAACATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACTCTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACTTGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAGCAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGTCATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTC  >  NC_000913/14462‑14935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑