Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,425 A→G P363P (CCA→CCG)  thrA → fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,4250AG96.8% 88.0 / NA 31P363P (CCA→CCG) thrAfused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (18/12);  minor base T (0/1);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

ACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGT  >  NC_000913/1189‑1664
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 
aCCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt                                                                                                                                                                                                                                     >  2:203279/1‑251 (MQ=255)
    gAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGa                                                                                                                                                                                                                                 <  2:142823/251‑1 (MQ=255)
                  ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa                                                                                                                                                                                                                    <  1:125756/250‑1 (MQ=255)
                                gggTGCCAGCCTGGATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGTTTGGCATGGCGGCGCGCGTGTTTGCAGCTATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTGCCGAAGACTGTATCAGTTTCTGCGTGCCTCAAAGCGACTGTGTGCGAGCTGAGCGGGCAATGCAGGAAGAGTTc                                                                                                                                                                                                      <  2:250914/249‑1 (MQ=255)
                                 ggTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCtc                                                                                                                                                                                                    >  1:27863/1‑250 (MQ=255)
                                   tGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGCGCTGAACGGGCAATGCAGGAAGAGTTCTAcc                                                                                                                                                                                                  >  2:103020/1‑251 (MQ=255)
                                       acccGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCGTGGTGGTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCCAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg                                                                                                                                                                                               >  2:187916/3‑250 (MQ=255)
                                                                                           tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATCGAGGCGCGCGTGGTTGCCGCGATGACACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAACAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGACGGGACGG‑CTGGCCAtt                                                                                                                                         >  1:181184/1‑251 (MQ=255)
                                                                                                  gTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGCGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGACCGGGCTGGC‑ATTTTCTCgg                                                                                                                                  >  1:135329/1‑251 (MQ=255)
                                                                                                        cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCggtggt                                                                                                                              >  1:65751/1‑249 (MQ=255)
                                                                                                                                gttGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGATGTCACGCGGCCGTACTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACCGTATCCGCTTCTTCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGTGTTCTACCTGGAACTGAACGAGGGCCTACTGGAGCCGCAGGCGGGGCAGGAACAG‑CCTGCCATTAACTCGGGGGTAggggagcgtattgcgcacatccgtg                                                                                                    >  2:72732/3‑228 (MQ=255)
                                                                                                                                   ggttggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAACGG‑CGGGCCCTTTACTCGGGGGGAGGGGGTGGGTTGCGCACCTTGCGTgggg                                                                                                 >  2:128914/5‑250 (MQ=255)
                                                                                                                                       ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCTCACCGTGCGTGGTAtctc                                                                                             <  1:79513/251‑1 (MQ=255)
                                                                                                                                                   cgcgtgattGCAGCACTGTCACGCGCCCGTATTTCAGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTTCGTGGGGTCTCGGCGAAATTCtt                                                                                 >  2:35778/8‑251 (MQ=255)
                                                                                                                                                      gtgtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGc                                                                              <  1:6113/248‑1 (MQ=255)
                                                                                                                                                        gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGcc                                                                             <  2:85857/249‑1 (MQ=255)
                                                                                                                                                                     tCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CCGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGGTCTCGGCGGAATTCTTTGCCGCGGTGGCCCccgc                                                               >  2:216942/1‑251 (MQ=255)
                                                                                                                                                                       aCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGcc                                                              >  1:183362/1‑250 (MQ=255)
                                                                                                                                                                         gcgcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGGATCAGTTTCTGCGTTCCGCAAAGCGACGGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGTCTTTCTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTGTGCCGCGCTGGCCCGCGCCAat                                                           <  2:143534/251‑1 (MQ=255)
                                                                                                                                                                               gTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAAc                                                     <  2:65751/251‑1 (MQ=255)
                                                                                                                                                                                 aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACA‑t                                                  >  1:99441/1‑251 (MQ=255)
                                                                                                                                                                                   ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACA‑tt                                                 <  1:216942/250‑1 (MQ=255)
                                                                                                                                                                                    tCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTCCTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGGGGTAGGCGTTGGTATGCGCACCTTGCGTGGGTTCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACA‑TTGt                                               >  2:18652/1‑251 (MQ=255)
                                                                                                                                                                                            gCTGATTACGCAATCATCTTGCGCATACAGGATCAGTTTCTGCGTTCCGCAAAGCGACTGGGTGCGAGCTGAACGGGCAATGCAGGAATAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCGTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCAGGCCCGCGCCAATATCCACA‑TTGTCGCCATTg                                       <  2:173914/251‑1 (MQ=255)
                                                                                                                                                                                                   aCGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGACGCTGGCGGTGAAGGAACGG‑CCGGCCATTATCTCGGTGGTAGGAGATGGTATTAGCACCTTGCGGGGGTGCTCGGAGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCCACA‑TTATCTCCATTGCTCAggg                                >  1:110444/1‑251 (MQ=255)
                                                                                                                                                                                                          catcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGCAACGG‑CTGGCCCTTATCCCGGTGGTAGGTGCTGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCACTATCAACA‑TTTTCGCCCTTTCTCAGGGAtcttct                         >  1:139935/1‑251 (MQ=255)
                                                                                                                                                                                                               tcgcaatacggtaTCAGCTTCTGCGTTCCGCAAGGCGACTGGGTGCGAGCTGAACGGGCAATTCAGGAATAGTTCTCCCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACCGAACGT‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCACATATCAACA‑TTGTCGCCATTGCTCAGTGATCTTCTg                        <  2:226251/235‑1 (MQ=255)
                                                                                                                                                                                                                         gtatccgTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAAAGG‑CTGGCCATTATATAGGTGGAAGGGGATGGTATGCGAACCCTGCGTGGGGTTTAGGCGAAATTTTTTGGCGCGCTGGGACGCGCCAATATCA‑CACTTGTCGCCAATGCTCGGGGCTCTTCTGAACCCTCaaattat          >  2:264113/7‑246 (MQ=255)
                                                                                                                                                                                                                             cAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGCAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATATCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCg      >  1:109980/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ttCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATCAACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTgg   <  1:68385/250‑1 (MQ=255)
                                                                                                                                                                                                                                  tCTGCGTTCCGCAAAGCGACGGTGTGCGAGCTGAGCGGGTAATGCAGGAAGAGTGCTACCTGGAACTGAAAGAAGGCTTACTGGGGCCGCTGGCGTTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGAGCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCATTAGCAGCA‑TTGTCGCGATTGCGCAGGGATCTTCTGAACGCTCAATCTTTGTCGTGGt  <  2:181184/250‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 
ACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATCAACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGTCGTGGT  >  NC_000913/1189‑1664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑