Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,846	G→T	G228G (GGG→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,846	0	G	T	97.1%	93.6 / NA	34	G228G (GGG→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (16/17);  minor base C (1/0);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAG  >  NC_000913/12614‑13093                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          gTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGc                                                                                                                                                                                                                                         >  2:57876/1‑251 (MQ=255)                                 ggTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTAGCGAAATCGACGAAGTTGACGGCGAAAAAACCGTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTGCGACAGCCGTCTGATCATCTACC‑TGGTTGAAGAATTc                                                                                                                                                                                                          <  2:43923/249‑1 (MQ=255)                                      ggAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGCTATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCaagaa                                                                                                                                                                                                     >  1:160212/1‑249 (MQ=255)                                            aaaaCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAg                                                                                                                                                                                              <  1:83185/250‑1 (MQ=255)                                               aCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGc                                                                                                                                                                                           >  1:146876/1‑250 (MQ=255)                                               aCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGc                                                                                                                                                                                           >  2:41739/1‑250 (MQ=255)                                                cGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCAt                                                                                                                                                                                         <  1:32130/251‑1 (MQ=255)                                                cGTATCATCAACGAACCGACCGCAGCGGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTGTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCAt                                                                                                                                                                                         <  1:106795/251‑1 (MQ=255)                                                            gAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCTGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTAGCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTGCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTg                                                                                                                                                                                                                  <  1:116004/214‑1 (MQ=255)                                                            gAACCGACCGCAGCTGCGCTGGCTTACGGCCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGCACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGCGGTGAAGACTTCGACAGCCGTCTGCTCAACTACC‑TGGTTg                                                                                                                                                                                                                  >  2:116004/1‑214 (MQ=255)                                                             aaCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCTCGTTTTATGACCTGGGTGGTTGTACTTTCGATATTTCTATTATCGAAATCGTCGATGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGTCTTCGACAGCCGTCTGATCAACT‑TCATGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAAc                                                                                                                                                                            <  1:35414/251‑1 (MQ=255)                                                                                  cTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgc                                                                                                                                                                                 >  2:230724/1‑225 (MQ=255)                                                                                  cTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgc                                                                                                                                                                                 <  1:230724/225‑1 (MQ=255)                                                                                            ggACAAGGGGACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGAGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATACGCTGGCAATGCAGCGC‑CTGAaaga                                                                                                                                              >  2:230247/1‑249 (MQ=255)                                                                                                    gtaCTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGaa                                                                                                                                    <  2:146876/249‑1 (MQ=255)                                                                                                    gtaCTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGa                                                                                                                                     >  1:27856/3‑250 (MQ=255)                                                                                                        tGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGaaaa                                                                                                                                  >  1:145439/1‑249 (MQ=255)                                                                                                         ggCAACCGTACTATCGCGGTTTAGGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTTGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAg                                                                                                                                <  2:117537/250‑1 (MQ=255)                                                                                                                  aCTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCg                                                                                                                       >  1:54769/1‑250 (MQ=255)                                                                                                                             ttctGACCTGGGAGGTGGTACTTTCGATATTTCTCTTATCGAACTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGACAACCAACGGTGATACCCACCTGGGTGGAGAAACCTTCGCCAGCCGTCTGATCAACTAAC‑TGGCTGAAGAATCAAAGAAAAATCAGAGCATAGAACTGCGGTAAGACCCGATGGCAATGC‑GGGCGCTGAAAAACGCGGGAAAAAAAGCGAACATCGAACtttagtc                                                                                                             >  2:197153/4‑243 (MQ=255)                                                                                                                             ttATGACCTGGGTGGGGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTcc                                                                                                            <  2:6393/250‑1 (MQ=255)                                                                                                                                  aCCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                                                                        >  2:135430/1‑249 (MQ=255)                                                                                                                                                                             cgaAGTTGACGGCGAAAACACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCACAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCg                                                           >  1:181896/1‑251 (MQ=255)                                                                                                                                                                             cgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTAAGCAGACCGACGTTAACCTGCCGAACATCACTGCAGATGCGCCCg                                                           >  2:232548/1‑251 (MQ=255)                                                                                                                                                                               aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGTTGCGACCGGt                                                         >  1:264800/1‑251 (MQ=255)                                                                                                                                                                                aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTc                                                        <  2:54769/251‑1 (MQ=255)                                                                                                                                                                                        gCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAacac                                                <  2:194534/251‑1 (MQ=255)                                                                                                                                                                                                 ccTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CGGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGGCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATc                                       <  1:41783/251‑1 (MQ=255)                                                                                                                                                                                                              ggCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCGCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTg                          <  2:34885/251‑1 (MQ=255)                                                                                                                                                                                                                       cGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTgg                 <  1:142385/251‑1 (MQ=255)                                                                                                                                                                                                                              aCCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGcc           <  1:41739/250‑1 (MQ=255)                                                                                                                                                                                                                                acACCTGGGTTGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGGTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAACAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGCAACACATGAACATCAACGTAACTCGGGCGAAACTGGAAAGACTgg        >  2:1811/2‑251 (MQ=255)                                                                                                                                                                                                                                  aCCTGGGTGGTGAATACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGCGACTCGTCCGAAACTGGAAATCCTGGtt      <  2:160212/251‑1 (MQ=255)                                                                                                                                                                                                                                    cTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTg     >  2:172567/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggggggTGAAGACTTCGACAGCCATCTGATCAACTACC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCAGTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACAGCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAg  <  2:100473/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          GTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATC‑TGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGC‑CTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAG  >  NC_000913/12614‑13093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑