Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,507	T→C	I115I (ATT→ATC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,507	0	T	C	100.0%	46.5 / NA	18	I115I (ATT→ATC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/7);  total (11/7)

ATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGAC  >  NC_000913/12280‑12744                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              aTTGACTATACCCAGGATGGTGAAACTGAGGTTGATCACCAGGCA‑ACACGTCAGCCAGTGACGAACACGCACAACACCATGATAGCGATTCCACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAGGGCCAGAAAAAGGCAACGCCGCAGATCACTGATGACGTGCTGataaacat                                                                                                                                                                                                                        >  2:35414/1‑248 (MQ=255)       tataCCCAGGATGGAGAAACTCTGGTTGGACAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGACAGAAAATGGAACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATgaaga                                                                                                                                                                                                                   >  2:228055/1‑250 (MQ=255)          accagagATGGAGAAACTCTGGTGGGTC‑GCCGGGTGAAACTACAGGAAGTGACGAACCCGCAAACCACCCTGTTTTGGATAAAACGCTTGATGGGCCGCCGCTTACAGGACGAAGAATTACAGCGTGATGTTTCCAGCATGCCGTTCAAATTGATGGCTGCTGATAACGGCGCCGCATGGGGAGAAGTTAAAGGAAAGAAAAGGACACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGaaaa                                                                                                                                                                                                                <  2:47926/244‑1 (MQ=255)                     gaaaaCTCTGGTGGGACAGCCGTCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGAAATTAAACGCATGATTGGCCTCCGTTTCCGGTACGAAAAATTACAGCGTGATTTTTCAATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGCCGCATGGTTCGAAGTAAAAGGACAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGAtt                                                                                                                                                                                                    <  1:89466/249‑1 (MQ=255)                             tggTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGAAAAACACCCTGTTTGCGATTACACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAACATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAACAAAATGAAGAAAACCGCTGAAGATTACCTGGGt                                                                                                                                                                                            >  2:46016/3‑251 (MQ=255)                                                                gAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCgg                                                                                                                                                          >  1:56033/1‑251 (MQ=255)                                                                        aaaaCACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACttt                                                                                                                                                  >  2:215488/1‑251 (MQ=255)                                                                                                                  ccaaGGACGAAGAATTACAGCGTGATGTTTCCATCAGGCCGGTCAAAATTATTGCTGCTAAGAACGGCGACGAAGGGTTCAAAGTAAAAGGCCAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTa                                                                                                          <  1:9831/246‑1 (MQ=255)                                                                                                                             gaagTACAGCGTGAGGTTTCCTGCAGGCCGTTAAAAATTATGGCTTCGGAGACGGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCTCCGTACCGGCATACTTTAACGATGCTCAGCGTCTGGCAACCAAAGACGCAGGCCGTATCGCTgtgctgga                                                                                             <  2:153900/251‑8 (MQ=255)                                                                                                                                 tACAGCGTGATGTTTCCATCAAGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAACAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTAGTCTGGAAGt                                                                                          >  2:57301/1‑250 (MQ=255)                                                                                                                                      cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACg                                                                                    >  1:16907/1‑251 (MQ=255)                                                                                                                                                    catGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAac                                                                      >  1:194534/1‑251 (MQ=255)                                                                                                                                                           gacAAAATTAGTGTTGCAGTTAACGGCGCCGTAGGGGTCGATGTAAAAGGCGAGAAATTGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAaccgaccg                                                                <  1:228055/248‑1 (MQ=255)                                                                                                                                                                           ctgATAAAGGCGACGCATGGGTCGAAGTTACAGGCCCGCAAATGCCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGACCCGGTAACTGACGCTGTTATCAACGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCCACGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGACCCGACCGCAGCTGCGATGGCTTAc                                               >  2:106795/1‑251 (MQ=255)                                                                                                                                                                                            ggggTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAggtac                              <  1:215488/250‑4 (MQ=255)                                                                                                                                                                                                                  aaaTGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGtt        >  2:83185/1‑251 (MQ=255)                                                                                                                                                                                                                    aTGGCACAGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATGACGAAAACCGCTGAAGTTTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGtttt      >  2:32130/1‑250 (MQ=255)                                                                                                                                                                                                                        cACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAGGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTAAGGTCTGGACAAAGGTACTGGCAACCGTACTACCGCGGTTTATGAc  <  2:49087/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGAC  >  NC_000913/12280‑12744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑