Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,357	T→C	T65T (ACT→ACC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,357	0	T	C	100.0%	47.7 / NA	18	T65T (ACT→ACC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/8);  total (10/8)

TACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTT  >  NC_000913/12114‑12600                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    tACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTg                                                                                                                                                                                                                                              >  1:194963/1‑251 (MQ=255)  aCAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACGCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTg                                                                                                                                                                                                                                              <  1:84552/250‑1 (MQ=255)                          cGAATATATAGTGGAGACGTGTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGccgccg                                                                                                                                                                                                                     <  1:150524/251‑1 (MQ=255)                                              ttAGATGGGTAAAATCATTGGTATCGCCCTGGGTACTACCAACTCTTGAGTAACGCCTATGGATAGAACCACTCCTCGTGTGCTGGCGAACACCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCCGGATGGTGAAAATCTGGTTGGACATCCAGCT‑AACCGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGaagaaggac                                                                                                                                                                                                 >  2:98714/1‑248 (MQ=255)                                               tAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTAc                                                                                                                                                                                                 <  2:253755/250‑1 (MQ=255)                                                                                    ccAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATtgct                                                                                                                                                           <  1:112125/251‑1 (MQ=255)                                                                                              tgtAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACg                                                                                                                                                 >  2:9831/1‑251 (MQ=255)                                                                                                            atggCACCACTCCTCGTGTGCTGGAGAACGCCGAATGCGATCGCACCACGCCTTCTATCAGTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGGCGCTTCCAGGACGAAGAAGGACAGCGTGATGTTTCCATCAGGCCGTGCAAAATTATTGCTGCTGATAACGGTTACTCATGGGTc                                                                                                                                   <  2:159033/251‑1 (MQ=255)                                                                                                                                         gCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAACTTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCAccgcc                                                                                                      >  1:12955/1‑251 (MQ=255)                                                                                                                                                     cGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAg                                                                                                                    <  2:225522/225‑1 (MQ=255)                                                                                                                                                     cGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAg                                                                                                                    >  1:225522/1‑225 (MQ=255)                                                                                                                                                                       aTTGACTATACCCAGGATGGTGAAACTGAGGTTGATCACCAGGCA‑ACACGTCAGCCAGTGACGAACACGCACAACACCATGATAGCGATTCCACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAGGGCCAGAAAAAGGCAACGCCGCAGATCACTGATGACGTGCTGataaacat                                                                        >  2:35414/1‑248 (MQ=255)                                                                                                                                                                             tataCCCAGGATGGAGAAACTCTGGTTGGACAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGACAGAAAATGGAACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATgaaga                                                                   >  2:228055/1‑250 (MQ=255)                                                                                                                                                                                accagagATGGAGAAACTCTGGTGGGTC‑GCCGGGTGAAACTACAGGAAGTGACGAACCCGCAAACCACCCTGTTTTGGATAAAACGCTTGATGGGCCGCCGCTTACAGGACGAAGAATTACAGCGTGATGTTTCCAGCATGCCGTTCAAATTGATGGCTGCTGATAACGGCGCCGCATGGGGAGAAGTTAAAGGAAAGAAAAGGACACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGaaaa                                                                <  2:47926/244‑1 (MQ=255)                                                                                                                                                                                           gaaaaCTCTGGTGGGACAGCCGTCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGAAATTAAACGCATGATTGGCCTCCGTTTCCGGTACGAAAAATTACAGCGTGATTTTTCAATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGCCGCATGGTTCGAAGTAAAAGGACAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGAtt                                                    <  1:89466/249‑1 (MQ=255)                                                                                                                                                                                                   tggTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGAAAAACACCCTGTTTGCGATTACACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAACATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAACAAAATGAAGAAAACCGCTGAAGATTACCTGGGt                                            >  2:46016/3‑251 (MQ=255)                                                                                                                                                                                                                                      gAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCgg          >  1:56033/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaaCACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACttt  >  2:215488/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTT  >  NC_000913/12114‑12600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑