BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,309	A→G	L49L (CTA→CTG)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,309	0	A	G	100.0%	56.2 / NA	20	L49L (CTA→CTG)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/13); total (7/13)

TGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATT  >  NC_000913/12066‑12550

ggCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTc                                                                                                                                                                                                                                             <  2:47701/250‑1 (MQ=255)

 gCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCa                                                                                                                                                                                                                                            <  1:114825/251‑1 (MQ=255)

     aaaaaTGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCg                                                                                                                                                                                                                                        <  1:117869/251‑1 (MQ=255)

                             ccAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAAc                                                                                                                                                                                                               <  1:81326/251‑1 (MQ=255)

                                           ccTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAacaccct                                                                                                                                                                                                 <  2:145211/251‑4 (MQ=255)

                                                tACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTg                                                                                                                                                                                            >  1:194963/1‑251 (MQ=255)

                                                 aCAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACGCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTg                                                                                                                                                                                            <  1:84552/250‑1 (MQ=255)

                                                                         cGAATATATAGTGGAGACGTGTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGccgccg                                                                                                                                                                   <  1:150524/251‑1 (MQ=255)

                                                                                             ttAGATGGGTAAAATCATTGGTATCGCCCTGGGTACTACCAACTCTTGAGTAACGCCTATGGATAGAACCACTCCTCGTGTGCTGGCGAACACCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCCGGATGGTGAAAATCTGGTTGGACATCCAGCT‑AACCGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGaagaaggac                                                                                                                                               >  2:98714/1‑248 (MQ=255)

                                                                                              tAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTAc                                                                                                                                               <  2:253755/250‑1 (MQ=255)

                                                                                                                                   ccAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATtgct                                                                                                         <  1:112125/251‑1 (MQ=255)

                                                                                                                                             tgtAGCGATTATGGATGGCACCACTCCTCGTGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACg                                                                                               >  2:9831/1‑251 (MQ=255)

                                                                                                                                                           atggCACCACTCCTCGTGTGCTGGAGAACGCCGAATGCGATCGCACCACGCCTTCTATCAGTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGGCGCTTCCAGGACGAAGAAGGACAGCGTGATGTTTCCATCAGGCCGTGCAAAATTATTGCTGCTGATAACGGTTACTCATGGGTc                                                                                 <  2:159033/251‑1 (MQ=255)

                                                                                                                                                                                        gCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAACTTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCAccgcc                                                    >  1:12955/1‑251 (MQ=255)

                                                                                                                                                                                                    cGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAg                                                                  >  1:225522/1‑225 (MQ=255)

                                                                                                                                                                                                    cGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAg                                                                  <  2:225522/225‑1 (MQ=255)

                                                                                                                                                                                                                      aTTGACTATACCCAGGATGGTGAAACTGAGGTTGATCACCAGGCA‑ACACGTCAGCCAGTGACGAACACGCACAACACCATGATAGCGATTCCACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAGGGCCAGAAAAAGGCAACGCCGCAGATCACTGATGACGTGCTGataaacat                      >  2:35414/1‑248 (MQ=255)

                                                                                                                                                                                                                            tataCCCAGGATGGAGAAACTCTGGTTGGACAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGACAGAAAATGGAACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATgaaga                 >  2:228055/1‑250 (MQ=255)

                                                                                                                                                                                                                               accagagATGGAGAAACTCTGGTGGGTC‑GCCGGGTGAAACTACAGGAAGTGACGAACCCGCAAACCACCCTGTTTTGGATAAAACGCTTGATGGGCCGCCGCTTACAGGACGAAGAATTACAGCGTGATGTTTCCAGCATGCCGTTCAAATTGATGGCTGCTGATAACGGCGCCGCATGGGGAGAAGTTAAAGGAAAGAAAAGGACACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGaaaa              <  2:47926/244‑1 (MQ=255)

                                                                                                                                                                                                                                          gaaaaCTCTGGTGGGACAGCCGTCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGAAATTAAACGCATGATTGGCCTCCGTTTCCGGTACGAAAAATTACAGCGTGATTTTTCAATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGCCGCATGGTTCGAAGTAAAAGGACAAAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGAtt  <  1:89466/249‑1 (MQ=255)

TGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAGCGATTATGGATGGCACCACTCCTCGCGTGCTGGAGAACGCCGAAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCT‑AAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATT  >  NC_000913/12066‑12550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`