Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,814	G→A	intergenic (‑28/‑349)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,814	0	G	A	100.0%	33.4 / NA	12	intergenic (‑28/‑349)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

GGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATG  >  NC_000913/11606‑12019                                                                                                                                                                                                                 |                                                                                                                                                                                                              ggTCGGCAGAGAGGTGTATGCGTTTCACCATGAGACAACCAGGCAGTTTCAGCGCCAGATCGTTGATTTCGTTACGCGGCATTGCAATGACGCCGAGGTGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGACATCGTAAAAAAGGATTTCATAAGGATTCTCTTAGTGGTCAGAGGTAGAGGGTTGAATACACACTAGTTTACTGCTGCTAAagagtaggtt                                                                                                                                                                     >  2:89466/1‑245 (MQ=255)           gagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTa                                                                                                                                                           <  2:59455/251‑1 (MQ=255)            agTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTGCAGCGCCAGATCGTTGGTTTCGTTACGCGGCAGTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAggttcggaa                                                                                                                                                          <  2:75349/251‑8 (MQ=255)                                gCGACAAACAGGAGGTTTCAGAGCCCGGTCGTTGGTTTCGTTACGCGGCATTGCAGTGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAAACGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGCTTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTTTTTTTATTACAAt                                                                                                                                      >  2:114825/1‑251 (MQ=255)                                                                                                              gTTTGCCTGCGCCGTGCAGCACAGCATCAGGCGAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCat                                                        <  2:151691/251‑1 (MQ=255)                                                                                                               tttGCCTGCGCCGTGCAGCACAGCATCAGGCTACTCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCat                                                        >  2:84552/1‑250 (MQ=255)                                                                                                                             gcagcaCAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTg                                         >  1:149111/1‑251 (MQ=255)                                                                                                                                                     ccggCTGGCGGAAATCGTAAAAACGGATTTCATACGGATTCTCTTAGTGGGAAGCGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAACTTTTTg                  >  2:228742/3‑250 (MQ=255)                                                                                                                                                         cTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCAtct             >  2:117869/1‑251 (MQ=255)                                                                                                                                                         cTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCAtct             >  2:81326/1‑251 (MQ=255)                                                                                                                                                           ggCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGGTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTcc           <  1:90857/251‑1 (MQ=255)                                                                                                                                                                    cGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATTAATACCCACTAGTTTACTGCTGATAAAGAGAAGTTTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTTATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTGTTGCATCTCCCCCTtgatg  <  1:228742/251‑1 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                              GGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTGTCAATTTTACGTCTTGTCCTGCCATATCGCGAAATTTCTGCGCAAAAGCACAAAAAATTTTTGCATCTCCCCCTTGATG  >  NC_000913/11606‑12019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑