Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,341	C→G	V335V (GTC→GTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,341	0	C	G	100.0%	66.4 / NA	25	V335V (GTC→GTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/9);  total (16/9)

GATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTC  >  NC_000913/1098‑1574                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           gATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGc                                                                                                                                                                                                                                       >  1:128877/1‑249 (MQ=255)     gctcTTTCCTACTGCGGCGCTAATGTTCTTCACCCCCCCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAACAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGTATTTCCGGTCAAGGGCATTTCCAATCTGAAGAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATTAAATCGATGGTTGGCATGGCGGCGCGCGTGTTGGCAGCGAt                                                                                                                                                                                                                                 <  2:160064/248‑1 (MQ=255)                                  tcACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATTCCTGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCTGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTg                                                                                                                                                                                                     <  1:236889/250‑1 (MQ=255)                                       cccGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACg                                                                                                                                                                                               >  1:85857/1‑251 (MQ=255)                                           cACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAat                                                                                                                                                                                           >  1:142823/1‑251 (MQ=255)                                                 tACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCtt                                                                                                                                                                                     >  1:89263/1‑251 (MQ=255)                                                        aTCGCCCAGGTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATa                                                                                                                                                                              <  1:237735/251‑1 (MQ=255)                                                                     aGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTCATGAAGACGCATTACCGGTCAAGGGAATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTCTTTCCGTGGAGCTGGTTACGCCATCATCTTCCGCATACAGTATCCGTTTc                                                                                                                                                                 >  1:254309/1‑251 (MQ=255)                                                                       aTCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTGCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGTCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAGCAGTTTCTg                                                                                                                                                               <  2:135321/251‑1 (MQ=255)                                                                        tCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTAAAGTATCAGTTTCTGc                                                                                                                                                              >  1:52144/1‑251 (MQ=255)                                                                              gCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTGTCTGCGTTccg                                                                                                                                                        <  2:20979/251‑2 (MQ=255)                                                                                cTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATTCAGTATCAGTTTCTGCGTTccgc                                                                                                                                                       >  1:175886/1‑248 (MQ=255)                                                                                            aCCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt                                                                                                                                          >  2:203279/1‑251 (MQ=255)                                                                                                gAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGa                                                                                                                                      <  2:142823/251‑1 (MQ=255)                                                                                                              ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa                                                                                                                         <  1:125756/250‑1 (MQ=255)                                                                                                                            gggTGCCAGCCTGGATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGTTTGGCATGGCGGCGCGCGTGTTTGCAGCTATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTGCCGAAGACTGTATCAGTTTCTGCGTGCCTCAAAGCGACTGTGTGCGAGCTGAGCGGGCAATGCAGGAAGAGTTc                                                                                                           <  2:250914/249‑1 (MQ=255)                                                                                                                             ggTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCtc                                                                                                         >  1:27863/1‑250 (MQ=255)                                                                                                                               tGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGCGCTGAACGGGCAATGCAGGAAGAGTTCTAcc                                                                                                       >  2:103020/1‑251 (MQ=255)                                                                                                                                   acccGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCGTGGTGGTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCCAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg                                                                                                    >  2:187916/3‑250 (MQ=255)                                                                                                                                                                                       tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATCGAGGCGCGCGTGGTTGCCGCGATGACACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAACAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGACGGGACGG‑CTGGCCAtt                                              >  1:181184/1‑251 (MQ=255)                                                                                                                                                                                              gTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGCGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGACCGGGCTGGC‑ATTTTCTCgg                                       >  1:135329/1‑251 (MQ=255)                                                                                                                                                                                                    cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCggtggt                                   >  1:65751/1‑249 (MQ=255)                                                                                                                                                                                                                            gttGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGATGTCACGCGGCCGTACTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACCGTATCCGCTTCTTCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGTGTTCTACCTGGAACTGAACGAGGGCCTACTGGAGCCGCAGGCGGGGCAGGAACAG‑CCTGCCATTAACTCGGGGGTAggggagcgtattgcgcacatccgtg         >  2:72732/3‑228 (MQ=255)                                                                                                                                                                                                                               ggttggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAACGG‑CGGGCCCTTTACTCGGGGGGAGGGGGTGGGTTGCGCACCTTGCGTgggg      >  2:128914/5‑250 (MQ=255)                                                                                                                                                                                                                                   ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCTCACCGTGCGTGGTAtctc  <  1:79513/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           GATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTC  >  NC_000913/1098‑1574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑