Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,973	T→A	K128N (AAA→AAT)  I48I (ATT→ATA)	yaaW ← mbiA →	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,973	0	T	A	96.4%	84.1 / NA	28	K128N (AAA→AAT)  I48I (ATT→ATA)	yaaW mbiA	putative enzyme‑specific chaperone YaaW uncharacterized protein MbiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (8/19);  minor base C (1/0);  total (9/19)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTT  >  NC_000913/10727‑11209                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gACCGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATAttc                                                                                                                                                                                                                                           <  2:102853/250‑1 (MQ=255)    cGCTTTAACCCCATTTAATGCTGCGCCCACAGGGCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTcc                                                                                                                                                                                                                                       >  1:84553/1‑251 (MQ=255)                                   gggCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTg                                                                                                                                                                                                        <  2:55433/251‑1 (MQ=255)                                    ggCCTCCCAGACCCGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGTAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGt                                                                                                                                                                                                       <  2:219371/251‑1 (MQ=255)                                      cctccaagagccgcgcagctCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATCTTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTg                                                                                                                                                                                                     >  2:196208/11‑251 (MQ=255)                                           cagacccGCGCCGCGCAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCaa                                                                                                                                                                                                <  1:191283/247‑1 (MQ=255)                                                   cgccgcgcAGCAAACCATGCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTCGCTCAATCTCAAAc                                                                                                                                                                                        >  2:36803/1‑251 (MQ=255)                                                                     gCCCAAGCACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTCGCTCAATCTCAAACGTAGACATCTCTTTGTc                                                                                                                                                                       <  1:141061/250‑1 (MQ=255)                                                                                      gctgcGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCg                                                                                                                                                     <  1:59995/251‑1 (MQ=255)                                                                                          cGTGGGTGCGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTCCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGtttt                                                                                                                                                 <  1:46231/251‑1 (MQ=255)                                                                                                  cGTAAAATGCGGGTCAATTGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATc                                                                                                                                         <  1:191306/251‑1 (MQ=255)                                                                                                      aactGCGGGTCAATTGGCTGGAAAGAAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGAAGCGGCAGTAGCTCTTCCAGCTCATACACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCCTATTATGCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATATATTTGTCGGATTTCAACTTCACTCGTTTTGAAACATctcgc                                                                                                                                     >  2:229840/4‑247 (MQ=255)                                                                                                                caattGGCTGGAAAGCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGcc                                                                                                                            <  1:187792/247‑1 (MQ=255)                                                                                                                                  aTGCGACACGCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTc                                                                                                         <  1:178301/251‑1 (MQ=255)                                                                                                                                                gtGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTttgttg                                                                                           >  2:769/2‑251 (MQ=255)                                                                                                                                                         atTTGTCTTTCATCAGCAGAGGCAGCAGCTCGTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTa                                                                                  <  1:192424/251‑1 (MQ=255)                                                                                                                                                               cTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGCTACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGcc                                                                             >  2:153167/1‑250 (MQ=255)                                                                                                                                                                 ttCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGccac                                                                           <  1:153167/250‑1 (MQ=255)                                                                                                                                                                 ttCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGGCGGCTTTCAGCTTCAAGCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGcc                                                                             <  2:89894/248‑1 (MQ=255)                                                                                                                                                                            cGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTCGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGATCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTa                                                               <  1:36803/251‑1 (MQ=255)                                                                                                                                                                                      tctTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCCGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTccagc                                                     >  2:192144/1‑248 (MQ=255)                                                                                                                                                                                                     ccccTGGCATCGACCGCGTTCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGtt                                      <  1:214346/250‑1 (MQ=255)                                                                                                                                                                                                      cccTGGCATCGATCGCGGGCAGAAACTCCTGCTTATGTTCCTCGTCCATACTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTTAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACAGCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATATTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTg                                     <  1:46181/251‑1 (MQ=255)                                                                                                                                                                                                       ccTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTgc                                    >  1:227389/1‑251 (MQ=255)                                                                                                                                                                                                                gACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGtt                           >  2:243487/1‑251 (MQ=255)                                                                                                                                                                                                                          aGAAACTCCTGCTTATGTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATg                 <  1:213044/251‑1 (MQ=255)                                                                                                                                                                                                                                    gCTTATGTTCCGCGTCCATATTCTGCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCGCTTTGGCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGATGTAAGTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCa         <  2:164611/249‑1 (MQ=255)                                                                                                                                                                                                                                          gTTCCTCGTCCATATTCTTCCAGGTGTTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATCTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGTTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAttt  <  1:242669/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACGCAGAAATTGTTCCAGTAACTGTTGCTCAATTTCAAACGTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTT  >  NC_000913/10727‑11209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑