Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323	C→T	V329V (GTC→GTT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323	0	C	T	100.0%	78.2 / NA	26	V329V (GTC→GTT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/9);  total (17/9)

AGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTG  >  NC_000913/1076‑1567                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTAAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGc                                                                                                                                                                                                                                                    >  1:173914/1‑251 (MQ=255)        gTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATggcgg                                                                                                                                                                                                                                             <  1:186235/251‑1 (MQ=255)                       gATGGAGTGTTCCTACTTCGGCGATAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCATCAAGCTCCCGGCACGCTAATTGGTGCAAGCTGTGATGAAGACGAATTACCGGTAAAGAGCATTTCCAATCTGAATAATATTGCAATATTCAGTGTTTCCGGCCCGTGGATGAAAGGGTTAGTTGGCATGGTGGtgcgcgggtttga                                                                                                                                                                                                                                >  2:194185/1‑242 (MQ=255)                       gATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGc                                                                                                                                                                                                                                >  1:128877/1‑249 (MQ=255)                           gctcTTTCCTACTGCGGCGCTAATGTTCTTCACCCCCCCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAACAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGTATTTCCGGTCAAGGGCATTTCCAATCTGAAGAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATTAAATCGATGGTTGGCATGGCGGCGCGCGTGTTGGCAGCGAt                                                                                                                                                                                                                          <  2:160064/248‑1 (MQ=255)                                                        tcACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATTCCTGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCTGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTg                                                                                                                                                                                              <  1:236889/250‑1 (MQ=255)                                                             cccGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACg                                                                                                                                                                                        >  1:85857/1‑251 (MQ=255)                                                                 cACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAat                                                                                                                                                                                    >  1:142823/1‑251 (MQ=255)                                                                       tACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCtt                                                                                                                                                                              >  1:89263/1‑251 (MQ=255)                                                                              aTCGCCCAGGTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATa                                                                                                                                                                       <  1:237735/251‑1 (MQ=255)                                                                                           aGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTCATGAAGACGCATTACCGGTCAAGGGAATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTCTTTCCGTGGAGCTGGTTACGCCATCATCTTCCGCATACAGTATCCGTTTc                                                                                                                                                          >  1:254309/1‑251 (MQ=255)                                                                                             aTCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTGCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGTCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAGCAGTTTCTg                                                                                                                                                        <  2:135321/251‑1 (MQ=255)                                                                                              tCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTAAAGTATCAGTTTCTGc                                                                                                                                                       >  1:52144/1‑251 (MQ=255)                                                                                                    gCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTGTCTGCGTTccg                                                                                                                                                 <  2:20979/251‑2 (MQ=255)                                                                                                      cTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATTCAGTATCAGTTTCTGCGTTccgc                                                                                                                                                >  1:175886/1‑248 (MQ=255)                                                                                                                  aCCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt                                                                                                                                   >  2:203279/1‑251 (MQ=255)                                                                                                                      gAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGa                                                                                                                               <  2:142823/251‑1 (MQ=255)                                                                                                                                    ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa                                                                                                                  <  1:125756/250‑1 (MQ=255)                                                                                                                                                  gggTGCCAGCCTGGATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGTTTGGCATGGCGGCGCGCGTGTTTGCAGCTATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTGCCGAAGACTGTATCAGTTTCTGCGTGCCTCAAAGCGACTGTGTGCGAGCTGAGCGGGCAATGCAGGAAGAGTTc                                                                                                    <  2:250914/249‑1 (MQ=255)                                                                                                                                                   ggTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCtc                                                                                                  >  1:27863/1‑250 (MQ=255)                                                                                                                                                     tGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGCGCTGAACGGGCAATGCAGGAAGAGTTCTAcc                                                                                                >  2:103020/1‑251 (MQ=255)                                                                                                                                                         acccGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCGTGGTGGTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCCAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg                                                                                             >  2:187916/3‑250 (MQ=255)                                                                                                                                                                                                             tGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATCGAGGCGCGCGTGGTTGCCGCGATGACACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAACAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGACGGGACGG‑CTGGCCAtt                                       >  1:181184/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGCGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGACCGGGCTGGC‑ATTTTCTCgg                                >  1:135329/1‑251 (MQ=255)                                                                                                                                                                                                                          cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGG‑CTGGCCATTATCTCggtggt                            >  1:65751/1‑249 (MQ=255)                                                                                                                                                                                                                                                  gttGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGATGTCACGCGGCCGTACTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACCGTATCCGCTTCTTCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGTGTTCTACCTGGAACTGAACGAGGGCCTACTGGAGCCGCAGGCGGGGCAGGAACAG‑CCTGCCATTAACTCGGGGGTAggggagcgtattgcgcacatccgtg  >  2:72732/3‑228 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      AGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGG‑CTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTG  >  NC_000913/1076‑1567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑