Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,006	G→A	S163S (AGC→AGT)	satP ←	acetate/succinate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,006	0	G	A	100.0%	67.5 / NA	23	S163S (AGC→AGT)	satP	acetate/succinate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

AACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAAT  >  NC_000913/9759‑10251                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aaCGTTGTGGAACCCGGTATTTTTGCGAGCATAACGTACTGCATTCCGTTGCTGAGAGGGCCATATGTTGAAACGGCAACAGCAGAGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAACAATCCCCCCGAGCGGGGGGATCTCAAAACAATTATTGGGATTCACCAATCCGACGAACGGAGCGACCAAACTGCTCGTGCAGTACTTAACCCATCGCCAAATAGATTGCACTg                                                                                                                                                                                                                                                    >  2:178783/1‑251 (MQ=255)   cGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGc                                                                                                                                                                                                                                                  >  2:129827/1‑251 (MQ=255)          gTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAg                                                                                                                                                                                                                                           <  2:216876/251‑1 (MQ=255)                           aaGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGTCACAAGAAGTGTTTGCTGCATTCAGACCGAAGAGTGCAAGACGAGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGTGATTCACCAATCTGCAGAACGGTGCGACCAAACTGCGCGTTCAGTAATTCACCCTTCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAgtc                                                                                                                                                                                                                          <  1:239652/250‑3 (MQ=255)                                               atgtGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAAGAAAAAACTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCCCGCTTCGCCATATAGATTGCACTTGCACCACAGATCATACCAATCCAGCCGGCACTTTGGATGATTgcgc                                                                                                                                                                                                      <  2:51056/248‑2 (MQ=255)                                                                           ggCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGCTTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGcc                                                                                                                                                                            >  1:250040/1‑249 (MQ=255)                                                                                                       ccGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAg                                                                                                                                              <  2:202358/251‑1 (MQ=255)                                                                                                          aaGAGTGCAAGACTCGACGGTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAAGCCAGCCGGCAAAGTTGATGAGTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGaaa                                                                                                                                           <  1:178783/251‑1 (MQ=255)                                                                                                          aaGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTGGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGaaa                                                                                                                                           <  2:1493/251‑1 (MQ=255)                                                                                                                gCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGaa                                                                                                                                      <  1:53004/250‑1 (MQ=255)                                                                                                                                           aaTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTACTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTg                                                                                                          >  1:258141/1‑251 (MQ=255)                                                                                                                                              ccccccGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCg                                                                                                       <  1:7898/251‑1 (MQ=255)                                                                                                                                                      gCGGGTGGATCTCAAAACAACTAGTGGGATTCACCAATCGGCAGCACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACTGCAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACAt                                                                                               <  1:12095/251‑1 (MQ=255)                                                                                                                                                         gggggATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACTGTGCGACCAAACTGCTCGTTCAGTACTTAACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATaa                                                                                             >  1:159634/1‑250 (MQ=255)                                                                                                                                                                     aaCAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATAc                                                                                >  1:96050/1‑251 (MQ=255)                                                                                                                                                                       cAATTAGTGGGATTCTCCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTCCTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGAGCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGc                                                                              <  1:246670/251‑1 (MQ=255)                                                                                                                                                                                          aTCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCATCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGac                                                           <  1:168214/251‑1 (MQ=255)                                                                                                                                                                                            cGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGacac                                                         >  2:60234/1‑251 (MQ=255)                                                                                                                                                                                            cGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGaca                                                          <  1:61719/250‑1 (MQ=255)                                                                                                                                                                                                aGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCATTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCa                                                       >  1:228848/1‑249 (MQ=255)                                                                                                                                                                                                                                       aTCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAAAAGCACAGTCAGGATAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCCAGGAACGGTGACTTGGGCGCATCGTGCAGAACCAGtttggg              >  2:214322/1‑248 (MQ=255)                                                                                                                                                                                                                                              gatagatTGCACTGACACCACAGATCAGACCTATCCCGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGACCTGTGCATTTGGCGCATCGGTCAGACCCCGTTTCGGCATcagc       >  2:99707/1‑251 (MQ=255)                                                                                                                                                                                                                                                   atTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACAGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAAt  >  1:260887/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      AACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCCAGCAGGGCAAACAGCACGGTCAGGCTAAAGAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTCGGCATCAGCAGAAT  >  NC_000913/9759‑10251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑