Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	9,851	A→T	A182A (GCA→GCT)	mog →	molybdopterin adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,851	0	A	T	100.0%	61.4 / NA	22	A182A (GCA→GCT)	mog	molybdopterin adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/14);  total (8/14)

GTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCC  >  NC_000913/9636‑10081                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       gTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTa                                                                                                                                                                                                      <  1:30955/250‑1 (MQ=255) gTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAAGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGACGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCAAGAGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGCCCGAAGAGACCAAGACGCGACGTTa                                                                                                                                                                                                      >  2:246670/1‑250 (MQ=255)     cAACTGCAATCCTTTCGCGTCAGGATGGTGTGCTTCGCAAACACGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGCGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGa                                                                                                                                                                                                  >  2:168214/1‑250 (MQ=255)       aCTGCGATCCTTTCGCGTCATGTTGGGGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCTGGTAACGTTTTGGTACACGGTATTTTTTCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAATGGTTGCTGCATTCAGACCGAAGAGTGCTAGACGCGACGGTAGCGAATa                                                                                                                                                                                               <  1:91017/251‑1 (MQ=255)                       gTCAGGTTGGTGTGATTCGCAAACAGGCGATGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCg                                                                                                                                                                                 >  1:91244/1‑249 (MQ=255)                         cAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTATCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTGGAAACGGCTCCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAAGCCCCCCGAGc                                                                                                                                                                              <  1:257330/250‑1 (MQ=255)                          aggttggtgTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCgg                                                                                                                                                                            <  2:113486/243‑1 (MQ=255)                                            aaCAGGCGCTGATCCTTAACGTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAtt                                                                                                                                                          <  2:182269/251‑1 (MQ=255)                                                                     cGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAAc                                                                                                                                 <  1:168099/251‑1 (MQ=255)                                                                                            agaCGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTa                                                                                                                                                                                                      <  2:63310/159‑1 (MQ=255)                                                                                            agaCGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTa                                                                                                                                                                                                      >  1:63310/1‑159 (MQ=255)                                                                                                    gAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCa                                                                                                  <  1:62378/251‑1 (MQ=255)                                                                                                          gtgAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCAt                                                                                             <  2:91244/250‑1 (MQ=255)                                                                                                                   gCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATGCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAAGTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACCGCTCGTTCAGTACTTCACCCATCGCCagatag                                                                                   <  2:193060/251‑1 (MQ=255)                                                                                                                            aaCGTTGTGGAACCCGGTATTTTTGCGAGCATAACGTACTGCATTCCGTTGCTGAGAGGGCCATATGTTGAAACGGCAACAGCAGAGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAACAATCCCCCCGAGCGGGGGGATCTCAAAACAATTATTGGGATTCACCAATCCGACGAACGGAGCGACCAAACTGCTCGTGCAGTACTTAACCCATCGCCAAATAGATTGCACTg                                                                          >  2:178783/1‑251 (MQ=255)                                                                                                                              cGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGc                                                                        >  2:129827/1‑251 (MQ=255)                                                                                                                                     gTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAg                                                                 <  2:216876/251‑1 (MQ=255)                                                                                                                                                      aaGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGTCACAAGAAGTGTTTGCTGCATTCAGACCGAAGAGTGCAAGACGAGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGTGATTCACCAATCTGCAGAACGGTGCGACCAAACTGCGCGTTCAGTAATTCACCCTTCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAgtc                                                <  1:239652/250‑3 (MQ=255)                                                                                                                                                                  cTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGc                                                                                                                            >  2:162884/1‑163 (MQ=255)                                                                                                                                                                  cTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGc                                                                                                                            <  1:162884/163‑1 (MQ=255)                                                                                                                                                                          atgtGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAAGAAAAAACTCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCCCGCTTCGCCATATAGATTGCACTTGCACCACAGATCATACCAATCCAGCCGGCACTTTGGATGATTgcgc                            <  2:51056/248‑2 (MQ=255)                                                                                                                                                                                                      ggCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGCTTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCACTGGCACCACAGATCAGACCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGcc  >  1:250040/1‑249 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       GTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGCGGGGGGATCTCAAAACAATTAGTGGGATTCACCAATCGGCAGAACGGTGCGACCAAACTGCTCGTTCAGTACTTCACCCATCGCCAGATAGATTGCGCTGGCACCGCAGATCAGCCCAATCCAGCCGGCAAAGTGGATGATTGCGGCGTTACCGGCAATGTTACCGATCGCC  >  NC_000913/9636‑10081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑