Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	9,668	C→T	G121G (GGC→GGT)	mog →	molybdopterin adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	9,668	0	C	T	96.0%	74.9 / NA	25	G121G (GGC→GGT)	mog	molybdopterin adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/10);  minor base G (0/1);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGC  >  NC_000913/9434‑9909                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  aaCCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCCGGTTGGTGTGATTCGCAAACAgg                                                                                                                                                                                                                                   >  2:30955/1‑251 (MQ=255)                                    aGCAAACGTTGTGTGAGCTTGTGGATGAAATGAGTTGCCATCTGTTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAATAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTGCCAACTGCGATCCTTTCGCGTCAAGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGACGAAGTCt                                                                                                                                                                                                <  1:197123/251‑1 (MQ=255)                                      ccaaCGTTGTATGGGCTAGTGGATGCAATAAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTACCGCCCTACGCGACGCCGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGCTGCGCCCGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGGCCGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTACAATTACCCGGTCCGCCGAAGTCTAt                                                                                                                                                                                              >  2:257330/3‑251 (MQ=255)                                                gtgAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTTGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGAc                                                                                                                                                                                     <  2:219494/250‑1 (MQ=255)                                                  gAGCTAGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCt                                                                                                                                                                                  <  1:108133/251‑1 (MQ=255)                                                     ctagtggATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGCACTGGCCCGGAGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGCAGGCTATTAAAGAGACGCTgg                                                                                                                                                                                >  2:32778/4‑250 (MQ=255)                                                                             cTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTATATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTa                                                                                                                                                        >  2:61719/1‑250 (MQ=255)                                                                               ggTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTa                                                                                                                                                        <  1:32778/248‑1 (MQ=255)                                                                                  gCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTtg                                                                                                                                                  <  2:233652/251‑1 (MQ=255)                                                                                     caccacGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTtgtg                                                                                                                                                <  1:45384/250‑1 (MQ=255)                                                                                                   cgggCCCGGGGCGTCGTGACGTAACGCCCGATGCGACGCTGGGAGTAGCTGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGAGCCTTAACGTGCCCGGTCAGCCGGAGTCTATTAAAGAGTCGCTGGAAGTTGTGAAGGACGCTTCGGGTAACGTTGTGGTACACGGTAttttt                                                                                                                                 <  2:23631/249‑1 (MQ=255)                                                                                                                          aCGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTc                                                                                                           >  1:216876/1‑250 (MQ=255)                                                                                                                               cGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGc                                                                                                     >  2:62378/1‑251 (MQ=255)                                                                                                                                    cGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGa                                                                                                 >  2:7898/1‑250 (MQ=255)                                                                                                                                        gCTGGCAGTAGCGGCCCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGc                                                                                            >  1:1493/1‑251 (MQ=255)                                                                                                                                                 aGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGtt                                                                                    <  2:187448/250‑1 (MQ=255)                                                                                                                                                          cgAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCAc                                                                          >  1:202358/1‑251 (MQ=255)                                                                                                                                                                      cTTTGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTg                                                              >  2:168099/1‑251 (MQ=255)                                                                                                                                                                         tGGTGAACAGATGCGCCAGATAAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTgctg                                                           >  1:113486/1‑249 (MQ=255)                                                                                                                                                                                                           gTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTa                          <  1:30955/250‑1 (MQ=255)                                                                                                                                                                                                           gTACCAACTGCGATCCTTTCGCGTCAGGTTGGTGTGATTCGCAAACAAGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGACGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCAAGAGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGCCCGAAGAGACCAAGACGCGACGTTa                          >  2:246670/1‑250 (MQ=255)                                                                                                                                                                                                               cAACTGCAATCCTTTCGCGTCAGGATGGTGTGCTTCGCAAACACGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGCGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGa                      >  2:168214/1‑250 (MQ=255)                                                                                                                                                                                                                 aCTGCGATCCTTTCGCGTCATGTTGGGGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCTGGTAACGTTTTGGTACACGGTATTTTTTCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAATGGTTGCTGCATTCAGACCGAAGAGTGCTAGACGCGACGGTAGCGAATa                   <  1:91017/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTCAGGTTGGTGTGATTCGCAAACAGGCGATGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCg     >  1:91244/1‑249 (MQ=255)                                                                                                                                                                                                                                   cAGGTTGGTGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGCGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTATCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTGGAAACGGCTCCAGAAGTGGTTGCTGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAAGCCCCCCGAGc  <  1:257330/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  AACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAGCTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGTCGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGTGAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTGGGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAAGAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCCAGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTGGTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAAAAAAATCCCCCCGAGC  >  NC_000913/9434‑9909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑