Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,131	T→C	L64L (TTG→CTG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,131	0	T	C	100.0%	29.9 / NA	12	L64L (TTG→CTG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

TTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATC  >  NC_000913/4640892‑4641359                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     ttGACAGTGATCACCCTGTCCGCGGTTTTTTGGTCATGAAGAGGGGCAATATGCGTATAACTATTCTTCTGGCCACACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAGGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTGCCCTACACTGGCTGAATc                                                                                                                                                                                                                            <  2:43912/250‑1 (MQ=255)  ggACAGTGATCCCCCTGTTCGGGGTTATTGGGTCAAGAAGAGTGGAAAGATGGGTATAACGATTATTCGGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGt                                                                                                                                                                                                                          <  2:67128/250‑1 (MQ=255)                       cgGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTg                                                                                                                                                                                                     <  1:67477/251‑1 (MQ=255)                                 gtCAAGAAGATTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCcaccac                                                                                                                                                                                           <  2:127714/250‑1 (MQ=255)                                             ggCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCg                                                                                                                                                                               >  2:194991/1‑251 (MQ=255)                                                           aaCGATTATTCTGGTCGCACCAGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGGCGCACATGGATCTGGGGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCCGTCGGGCGAAATATCAtt                                                                                                                                                                 >  2:56539/1‑251 (MQ=255)                                                                       ggTCGCACCCGCCAGAGCAGAACATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCCTTACtacgctacgc                                                                                                                                                     >  2:184555/1‑251 (MQ=255)                                                                                               tatTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCTCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGtt                                                                                                                               >  1:40870/1‑249 (MQ=255)                                                                                                                       gAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGCATCGTTACACGATGTCGATTTCACTGTCGCCACCAATGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGACAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGcc                                                                                                     >  1:35517/1‑251 (MQ=255)                                                                                                                                           aacTGCGGTTTGGCGATAGTCAGGCACTCCTGGAGCCATCCACCCGCTGGGTCGCACATGGATCTGGTTATATTATTGATAATATTAAAGTTTTCCCGACACGGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTGAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGt                                                                                 <  2:174662/249‑1 (MQ=255)                                                                                                                                                                   cacaCCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAAc                                                         <  1:86906/251‑1 (MQ=255)                                                                                                                                                                                                                          taataTTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATc  >  2:84563/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     TTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATC  >  NC_000913/4640892‑4641359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑