Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,022	G→T	G27G (GGG→GGT)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,022	0	G	T	100.0%	36.5 / NA	13	G27G (GGG→GGT)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/7);  total (6/7)

AACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCC  >  NC_000913/4640780‑4641260                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               aacaatTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGa                                                                                                                                                                                                                                        <  1:73412/251‑1 (MQ=255)                                                                                  aaggaTTTGTAAATTCGCAACGCGTAATAAGTTGACAGTGATCCCCCTGTTCGCGGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGata                                                                                                                                                        <  2:210302/246‑1 (MQ=255)                                                                                                                 ttGACAGTGATCACCCTGTCCGCGGTTTTTTGGTCATGAAGAGGGGCAATATGCGTATAACTATTCTTCTGGCCACACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAGGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTGCCCTACACTGGCTGAATc                                                                                                                         <  2:43912/250‑1 (MQ=255)                                                                                                                  ggACAGTGATCCCCCTGTTCGGGGTTATTGGGTCAAGAAGAGTGGAAAGATGGGTATAACGATTATTCGGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGt                                                                                                                       <  2:67128/250‑1 (MQ=255)                                                                                                                                       cgGTTATTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTg                                                                                                  <  1:67477/251‑1 (MQ=255)                                                                                                                                             tttGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATattatt                                                                                                                                                            >  1:34187/1‑187 (MQ=255)                                                                                                                                             tttGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATattatt                                                                                                                                                            <  2:34187/187‑1 (MQ=255)                                                                                                                                                 gtCAAGAAGATTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCcaccac                                                                                        <  2:127714/250‑1 (MQ=255)                                                                                                                                                             ggCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCg                                                                            >  2:194991/1‑251 (MQ=255)                                                                                                                                                                           aaCGATTATTCTGGTCGCACCAGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGGCGCACATGGATCTGGGGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCCGTCGGGCGAAATATCAtt                                                              >  2:56539/1‑251 (MQ=255)                                                                                                                                                                                       ggTCGCACCCGCCAGAGCAGAACATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCCTTACtacgctacgc                                                  >  2:184555/1‑251 (MQ=255)                                                                                                                                                                                                               tatTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCTCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGtt                            >  1:40870/1‑249 (MQ=255)                                                                                                                                                                                                                                       gAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACACTGGCTGCATCGTTACACGATGTCGATTTCACTGTCGCCACCAATGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGACAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGcc  >  1:35517/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               AACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCC  >  NC_000913/4640780‑4641260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑