Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,996	G→T	A253A (GCG→GCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,996	0	G	T	100.0%	71.2 / NA	24	A253A (GCG→GCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/13);  total (11/13)

GATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC  >  NC_000913/8759‑9202                                                                                                                                                                                                                                              |                                                                                                                                                                                                               gATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAgg                                                                                                                                                                                                     <  1:86104/249‑1 (MQ=255)  aTCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGc                                                                                                                                                                                                  >  1:30443/1‑251 (MQ=255)            ttgttgGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTAGCGAACg                                                                                                                                                                                         <  1:203907/250‑1 (MQ=255)                       aTTCTTGACTGGTCCAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTGTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCtt                                                                                                                                                                              <  1:13548/250‑1 (MQ=255)                                                     aagaaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGa                                                                                                                                                                                            >  2:68798/1‑206 (MQ=255)                                                     aagaaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGa                                                                                                                                                                                            <  1:68798/206‑1 (MQ=255)                                                        aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACAGCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAACGCTTCCTGCGCGTAt                                                                                                                                            <  2:257379/251‑1 (MQ=255)                                                            agTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACt                                                                                                                                        >  1:3869/1‑251 (MQ=255)                                                                                     cGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcac                                                                                                                >  1:231215/1‑250 (MQ=255)                                                                                                          cTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCCTCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACAAGGATACAATGGCAGt                                                                                            >  2:228710/1‑249 (MQ=255)                                                                                                                   ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGAGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGACAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTCGATAAACTgg                                                                                 >  2:200221/1‑251 (MQ=255)                                                                                                                              aCGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATc                                                                      <  2:15881/251‑1 (MQ=255)                                                                                                                                 gtgaTGAAACCGGGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTTCGACCGTCTTACCATCGCACCTACACTGCTGAAATAGCTGGCTGAGATCGAATGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCTTATCACTTAGTCCGAGTTCCGGTGGCAGTACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                   <  2:260832/248‑1 (MQ=255)                                                                                                                                        aaCCGTGGGTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTg                                                            <  2:3869/251‑1 (MQ=255)                                                                                                                                                    gggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAgg                                                <  1:200221/251‑1 (MQ=255)                                                                                                                                                     ggCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGCAAGAGCTGGCTGAGAGCGAAGGGGCTATCGACCGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTGGATCAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAACAgg                                                >  1:239293/1‑250 (MQ=255)                                                                                                                                                                       aTCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTATACTGCTGAAAGAGCTGGCTGAGAACGAAGGAACTATCGAACGTAAACTGTCTTAAACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCCGGATCCAATGGCAGTAGATAAACTGGCGGCAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAt                             >  1:263659/1‑251 (MQ=255)                                                                                                                                                                                 gtCTGGAACTGGCAGACTGCGACCGTCTTACCATCGCACCTACACTCATGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGAATTACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTCGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAACACTGGAAAAAATTATCGGCGATctg                   >  2:45384/2‑251 (MQ=255)                                                                                                                                                                                   cTGGAACTGGCAGGCTGCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgct                 >  1:160282/1‑251 (MQ=255)                                                                                                                                                                                             cAGGCTACTACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTTGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTTCACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAATCTGTCGGAAGGGGTCCGTAAGATTGCTATTGACCAGTAAAAACTGAAAAAAATGATCGGTGATCTGCTGTAATCATTc       <  2:251037/251‑1 (MQ=255)                                                                                                                                                                                                   gCGACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGGCCGGCGCGTATCACTGTGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGc  <  1:246471/250‑1 (MQ=255)                                                                                                                                                                                                     gACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                   >  2:143566/1‑183 (MQ=255)                                                                                                                                                                                                     gACCGTCTTACCATCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                   <  1:143566/183‑1 (MQ=255)                                                                                                                                                                                                                 aTCGCACCTACACTGCTGAAAGAGCTGGCTGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTg                                                            <  1:113670/178‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                               GATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGC  >  NC_000913/8759‑9202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑