Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,608	G→A	C233C (TGC→TGT)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,608	0	G	A	100.0%	33.6 / NA	13	C233C (TGC→TGT)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/3);  total (10/3)

GTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCG  >  NC_000913/4639402‑4639839                                                                                                                                                                                                               |                                                                                                                                                                                                                                        gtgCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccgg                                                                                                                                                                                             <  1:54295/251‑1 (MQ=255)                                             tatCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCg                                                                                                                                                 >  2:65775/1‑251 (MQ=255)                                                            gTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGTATACGGCGGATCGTCACGTCtcaagta                                                                                                                                   >  2:100491/1‑244 (MQ=255)                                                                          aaaTGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATGTGGCAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGAGGTAAAGCCGATTAATCTTCCAGATCACAACAGAAGCGATAACCTTCACCGTGGCTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGAGTGGTTTc                                                                                                                    >  1:35677/1‑251 (MQ=255)                                                                                                 acaatggacgctcaattACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGCCATTttctt                                                                                             >  1:54356/16‑251 (MQ=255)                                                                                                             caattACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTccgc                                                                                 >  1:211484/4‑248 (MQ=255)                                                                                                                           gAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTACTCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGCTTCGAAATGTTTa                                                                                                                                                                 >  1:268128/1‑157 (MQ=255)                                                                                                                           gAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTGTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGAGTCGAAATGTTTa                                                                                                                                                                 <  2:268128/157‑1 (MQ=255)                                                                                                                                           aTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTcac                                                    >  1:73401/1‑250 (MQ=255)                                                                                                                                                aaCGGCGCTCAAAAACGCCGTCTTTTTTGCCGGAGGAAAAGCCGCTTAATCTTCCAGTTCACCACAGCAGCGATACCCTTCACCGTGGATGGTGGCGCTGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGCATCCGACGGATCGTCACGTCTACAGGACGGTCTTGTGGATTCAGCTCACGGCCCGTCATTTTTTTCAGCAGTTCGACACGAGGCTTGATTTTGCCTggagtttctccgcagt                                              >  2:162149/1‑237 (MQ=255)                                                                                                                                                                                            aTTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCg  >  1:21717/1‑251 (MQ=255)                                                                                                                                                                                                      cAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTg      >  1:12920/1‑237 (MQ=255)                                                                                                                                                                                                      cAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTg      <  2:12920/237‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                        GTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCG  >  NC_000913/4639402‑4639839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑