Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,303	T→C	C376R (TGC→CGC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,303	0	T	C	100.0%	46.5 / NA	20	C376R (TGC→CGC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/14);  total (6/14)

CCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAA  >  NC_000913/4639056‑4639543                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccGGGCGGCTAAGGACGCCATGTTGCTGATTGCACTGACTTTTATGGCGGCCTATGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGTGGTTTTCATTGGTGATGTTTTATTTGCTCGTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCa                                                                                                                                                                                                                                               <  2:248265/251‑1 (MQ=255)               aCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAGTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATgttgtt                                                                                                                                                                                                                                  <  1:166443/250‑1 (MQ=255)                                         tttATGGCGTCCTTTTGTTTTGAAACGCTCCCCTCGCAACGTTTACACCCAATGCAATATTTGCTGGGGGGGCTTTCATTGGTGAGGTTGTATTTGCTCTTTCCGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCTGTATTGTAAGGCTGGCTCATCAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGAtt                                                                                                                                                                                                        <  2:130826/250‑2 (MQ=255)                                                            ttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCt                                                                                                                                                                                     <  1:169435/250‑1 (MQ=255)                                                                      cACCGCGCCACGTTGACCCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAGGTTTTTTTTGCTCTTGCTGGCTCTTGCTGATCATATCGGTTTTCCTGTGGCATGGATAAGCGCCAGTCTGATTGGGTCGTTAATGAACGGTAGTTATTTGCCGGCGGTATTGAAAGGCTGTCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGTATGGTGTGATTTTGGGACTGATCAACTCTGcc                                                                                                                                                                           <  2:221163/250‑1 (MQ=255)                                                                                                aaTATTTGCTGGTGGGGCTTTCATTGGTGCTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgg                                                                                                                                                >  1:210306/1‑250 (MQ=255)                                                                                                         tggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTg                                                                                                                                                                             <  1:139624/213‑1 (MQ=255)                                                                                                          ggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATAGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGAGGAATTGAAAGGCGGGCGGAACAGCAAGTTGTTTAACCTCGCGGAGTTGGTGCTGGAAGGGGTGATGGGGGGACTGCTCAACGCTGCCGATAGCGAGCTGGTGGTGGGAACCAgggaggtggggg                                                                                                                                      >  1:138605/1‑240 (MQ=255)                                                                                                              gggcgttgaTTGGTGATGTTTTTGGTTCTCTTGCTGGCGCTTGTAGAACATATCGGTTTTACCGGGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGACGTGGGGACTGCTCAACTCTGCCGATAGCGCGCGGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                                                                                                                  <  2:100154/243‑1 (MQ=255)                                                                                                              gggCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTTCTGGCTGGTGTGGTGTGGGGACTGATCACCTTTGCCGATTGCGCGCTGTTGTTGGGTACCAGGTTGCTGGTTGTGgc                                                                                                                                  >  2:109539/1‑251 (MQ=255)                                                                                                                   ttCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCGGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCATTGTGCTGGTGGTGGCGCTg                                                                                                                              <  2:188723/250‑1 (MQ=255)                                                                                                                    tCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGGGGCGCTGGc                                                                                                                            >  1:210832/1‑251 (MQ=255)                                                                                                                                                               atatcGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGGAACAGTGTGCTGGGGGGGGCGCTGGCCGGCATGATGTTTTGGACTCGTTATATTGACTGGGATGCGttt                                                                                 >  2:125585/5‑251 (MQ=255)                                                                                                                                                                             gTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAGCAGCATTTTGTTTACCCGCGCGCTGTTGTTGCTGGATGGTGTTATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGTAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAAt                                                                   <  1:268756/251‑1 (MQ=255)                                                                                                                                                                                                            ggttAATGATCGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGAGGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTTGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGAt                                    <  2:175911/248‑1 (MQ=255)                                                                                                                                                                                                                     aCGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTGCCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGt                           <  1:109539/251‑1 (MQ=255)                                                                                                                                                                                                                           tttatttGCAGGCGGTATGGAAAGTCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTGGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGTCCGGCATGATGTTTGTGACTCGTAATATCGGCTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTg                      <  2:138605/250‑1 (MQ=255)                                                                                                                                                                                                                                        ggTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGa         >  2:54295/1‑250 (MQ=255)                                                                                                                                                                                                                                              gAAAGGCTGGCGCAACAGCATTTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa  <  2:210306/251‑1 (MQ=255)                                                                                                                                                                                                                                              gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa  <  1:125585/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAA  >  NC_000913/4639056‑4639543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑