Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,284	A→G	Q369Q (CAA→CAG)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,284	0	A	G	100.0%	46.5 / NA	18	Q369Q (CAA→CAG)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/12);  total (6/12)

ATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTG  >  NC_000913/4639041‑4639523                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                aTACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCCACGTTTACACCCAATGCCATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGCACATATCGGTTTTACCGTGGCATGGCTAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTAt                                                                                                                                                                                                                                          >  2:229656/1‑251 (MQ=255)                ccGGGCGGCTAAGGACGCCATGTTGCTGATTGCACTGACTTTTATGGCGGCCTATGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGTGGTTTTCATTGGTGATGTTTTATTTGCTCGTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCa                                                                                                                                                                                                                           <  2:248265/251‑1 (MQ=255)                              aCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAGTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATgttgtt                                                                                                                                                                                                              <  1:166443/250‑1 (MQ=255)                                                        tttATGGCGTCCTTTTGTTTTGAAACGCTCCCCTCGCAACGTTTACACCCAATGCAATATTTGCTGGGGGGGCTTTCATTGGTGAGGTTGTATTTGCTCTTTCCGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCTGTATTGTAAGGCTGGCTCATCAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGAtt                                                                                                                                                                                    <  2:130826/250‑2 (MQ=255)                                                                           ttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCt                                                                                                                                                                 <  1:169435/250‑1 (MQ=255)                                                                                     cACCGCGCCACGTTGACCCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAGGTTTTTTTTGCTCTTGCTGGCTCTTGCTGATCATATCGGTTTTCCTGTGGCATGGATAAGCGCCAGTCTGATTGGGTCGTTAATGAACGGTAGTTATTTGCCGGCGGTATTGAAAGGCTGTCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGTATGGTGTGATTTTGGGACTGATCAACTCTGcc                                                                                                                                                       <  2:221163/250‑1 (MQ=255)                                                                                                               aaTATTTGCTGGTGGGGCTTTCATTGGTGCTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgg                                                                                                                            >  1:210306/1‑250 (MQ=255)                                                                                                                        tggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTg                                                                                                                                                         <  1:139624/213‑1 (MQ=255)                                                                                                                         ggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATAGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGAGGAATTGAAAGGCGGGCGGAACAGCAAGTTGTTTAACCTCGCGGAGTTGGTGCTGGAAGGGGTGATGGGGGGACTGCTCAACGCTGCCGATAGCGAGCTGGTGGTGGGAACCAgggaggtggggg                                                                                                                  >  1:138605/1‑240 (MQ=255)                                                                                                                             gggcgttgaTTGGTGATGTTTTTGGTTCTCTTGCTGGCGCTTGTAGAACATATCGGTTTTACCGGGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGACGTGGGGACTGCTCAACTCTGCCGATAGCGCGCGGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                                                                                              <  2:100154/243‑1 (MQ=255)                                                                                                                             gggCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTTCTGGCTGGTGTGGTGTGGGGACTGATCACCTTTGCCGATTGCGCGCTGTTGTTGGGTACCAGGTTGCTGGTTGTGgc                                                                                                              >  2:109539/1‑251 (MQ=255)                                                                                                                                  ttCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCGGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCATTGTGCTGGTGGTGGCGCTg                                                                                                          <  2:188723/250‑1 (MQ=255)                                                                                                                                   tCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGGGGCGCTGGc                                                                                                        >  1:210832/1‑251 (MQ=255)                                                                                                                                                                              atatcGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGGAACAGTGTGCTGGGGGGGGCGCTGGCCGGCATGATGTTTTGGACTCGTTATATTGACTGGGATGCGttt                                                             >  2:125585/5‑251 (MQ=255)                                                                                                                                                                                            gTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAGCAGCATTTTGTTTACCCGCGCGCTGTTGTTGCTGGATGGTGTTATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGTAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAAt                                               <  1:268756/251‑1 (MQ=255)                                                                                                                                                                                                                           ggttAATGATCGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGAGGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTTGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGAt                <  2:175911/248‑1 (MQ=255)                                                                                                                                                                                                                                    aCGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTGCCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGt       <  1:109539/251‑1 (MQ=255)                                                                                                                                                                                                                                          tttatttGCAGGCGGTATGGAAAGTCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTGGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGTCCGGCATGATGTTTGTGACTCGTAATATCGGCTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTg  <  2:138605/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                ATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTG  >  NC_000913/4639041‑4639523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑