Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,261	A→T	I362L (ATA→TTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,261	0	A	T	100.0%	57.7 / NA	20	I362L (ATA→TTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/12);  total (8/12)

GTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAAT  >  NC_000913/4639015‑4639478                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          gtcgccgtgaccacgCCAGCCGATCAATAACAATTACCGAACCGGGCGACTAAGTACGCCATTCAGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGCAACGCACACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt                                                                                                                                                                                                                       >  2:268756/13‑251 (MQ=255) gTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGTTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt                                                                                                                                                                                                                       <  1:164379/251‑1 (MQ=255)            gacgCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattt                                                                                                                                                                                                            <  1:152266/251‑1 (MQ=255)             acgCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattta                                                                                                                                                                                                           <  1:131685/251‑1 (MQ=255)                     cGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCagg                                                                                                                                                                                                   >  1:175911/1‑249 (MQ=255)                           aTACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCCACGTTTACACCCAATGCCATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGCACATATCGGTTTTACCGTGGCATGGCTAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTAt                                                                                                                                                                                             >  2:229656/1‑251 (MQ=255)                                          ccGGGCGGCTAAGGACGCCATGTTGCTGATTGCACTGACTTTTATGGCGGCCTATGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGTGGTTTTCATTGGTGATGTTTTATTTGCTCGTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCa                                                                                                                                                                              <  2:248265/251‑1 (MQ=255)                                                        aCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAGTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATgttgtt                                                                                                                                                                 <  1:166443/250‑1 (MQ=255)                                                                                  tttATGGCGTCCTTTTGTTTTGAAACGCTCCCCTCGCAACGTTTACACCCAATGCAATATTTGCTGGGGGGGCTTTCATTGGTGAGGTTGTATTTGCTCTTTCCGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCTGTATTGTAAGGCTGGCTCATCAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGAtt                                                                                                                                       <  2:130826/250‑2 (MQ=255)                                                                                                     ttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCt                                                                                                                    <  1:169435/250‑1 (MQ=255)                                                                                                               cACCGCGCCACGTTGACCCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAGGTTTTTTTTGCTCTTGCTGGCTCTTGCTGATCATATCGGTTTTCCTGTGGCATGGATAAGCGCCAGTCTGATTGGGTCGTTAATGAACGGTAGTTATTTGCCGGCGGTATTGAAAGGCTGTCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGTATGGTGTGATTTTGGGACTGATCAACTCTGcc                                                                                                          <  2:221163/250‑1 (MQ=255)                                                                                                                                         aaTATTTGCTGGTGGGGCTTTCATTGGTGCTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgg                                                                               >  1:210306/1‑250 (MQ=255)                                                                                                                                                  tggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTg                                                                                                            <  1:139624/213‑1 (MQ=255)                                                                                                                                                   ggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATAGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGAGGAATTGAAAGGCGGGCGGAACAGCAAGTTGTTTAACCTCGCGGAGTTGGTGCTGGAAGGGGTGATGGGGGGACTGCTCAACGCTGCCGATAGCGAGCTGGTGGTGGGAACCAgggaggtggggg                                                                     >  1:138605/1‑240 (MQ=255)                                                                                                                                                       gggcgttgaTTGGTGATGTTTTTGGTTCTCTTGCTGGCGCTTGTAGAACATATCGGTTTTACCGGGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGACGTGGGGACTGCTCAACTCTGCCGATAGCGCGCGGTTGTTGGGAACCAGTGTGCTGGTGGTGgc                                                                 <  2:100154/243‑1 (MQ=255)                                                                                                                                                       gggCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTTCTGGCTGGTGTGGTGTGGGGACTGATCACCTTTGCCGATTGCGCGCTGTTGTTGGGTACCAGGTTGCTGGTTGTGgc                                                                 >  2:109539/1‑251 (MQ=255)                                                                                                                                                            ttCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCGGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCATTGTGCTGGTGGTGGCGCTg                                                             <  2:188723/250‑1 (MQ=255)                                                                                                                                                             tCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGGGGCGCTGGc                                                           >  1:210832/1‑251 (MQ=255)                                                                                                                                                                                                        atatcGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGGAACAGTGTGCTGGGGGGGGCGCTGGCCGGCATGATGTTTTGGACTCGTTATATTGACTGGGATGCGttt                >  2:125585/5‑251 (MQ=255)                                                                                                                                                                                                                      gTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAGCAGCATTTTGTTTACCCGCGCGCTGTTGTTGCTGGATGGTGTTATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGTAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAAt  <  1:268756/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          GTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAAT  >  NC_000913/4639015‑4639478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑