Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,217	C→T	T347I (ACC→ATC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,217	0	C	T	100.0%	67.2 / NA	25	T347I (ACC→ATC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

CAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGC  >  NC_000913/4638974‑4639421                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             cAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGGGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGGGATGGTTTATTTGCTCTTGCTGTCGCTTTCTGAACAGATCGGttt                                                                                                                                                                                                        <  2:50687/250‑1 (MQ=255)             ccGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGttt                                                                                                                                                                                                        >  1:104014/1‑238 (MQ=255)             ccGGCTGGGAAAACTTCCCCGCGGTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATGCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCGCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGttt                                                                                                                                                                                                        <  2:104014/238‑1 (MQ=255)              cGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGCCGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGATGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATgg                                                                                                                                                                                           >  2:131685/1‑250 (MQ=255)                   gggAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTCCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATCTGCTGGTGGGGTTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATc                                                                                                                                                                                     >  1:100154/1‑251 (MQ=255)                           ttCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGCTCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTTCTGACGCTTTCTGAACATATCGGTTTTACCGAGGCATGGATAATCGCCAGTCt                                                                                                                                                                             >  1:248265/1‑251 (MQ=255)                              ccctcGTTTAGCGTCGCAGTGACGCCGCCAGCCGAGCAATACCAATTAACGGACCGGGCGACTACGTACGCCATTCTGCTGATTGCTCTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCTCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCCTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAt                                                                                                                                                                          <  1:109785/247‑1 (MQ=255)                                          gtcgccgtgaccacgCCAGCCGATCAATAACAATTACCGAACCGGGCGACTAAGTACGCCATTCAGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGCAACGCACACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt                                                                                                                                                              >  2:268756/13‑251 (MQ=255)                                          gTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGTTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt                                                                                                                                                              <  1:164379/251‑1 (MQ=255)                                                     gacgCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattt                                                                                                                                                   <  1:152266/251‑1 (MQ=255)                                                      acgCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGtattta                                                                                                                                                  <  1:131685/251‑1 (MQ=255)                                                              cGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCagg                                                                                                                                          >  1:175911/1‑249 (MQ=255)                                                                    aTACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCCACGTTTACACCCAATGCCATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGCACATATCGGTTTTACCGTGGCATGGCTAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTAt                                                                                                                                    >  2:229656/1‑251 (MQ=255)                                                                                   ccGGGCGGCTAAGGACGCCATGTTGCTGATTGCACTGACTTTTATGGCGGCCTATGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGTGGTTTTCATTGGTGATGTTTTATTTGCTCGTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCa                                                                                                                     <  2:248265/251‑1 (MQ=255)                                                                                                 aCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAGTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATgttgtt                                                                                                        <  1:166443/250‑1 (MQ=255)                                                                                                                           tttATGGCGTCCTTTTGTTTTGAAACGCTCCCCTCGCAACGTTTACACCCAATGCAATATTTGCTGGGGGGGCTTTCATTGGTGAGGTTGTATTTGCTCTTTCCGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCTGTATTGTAAGGCTGGCTCATCAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGAtt                                                                              <  2:130826/250‑2 (MQ=255)                                                                                                                                              ttGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCt                                                           <  1:169435/250‑1 (MQ=255)                                                                                                                                                        cACCGCGCCACGTTGACCCCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAGGTTTTTTTTGCTCTTGCTGGCTCTTGCTGATCATATCGGTTTTCCTGTGGCATGGATAAGCGCCAGTCTGATTGGGTCGTTAATGAACGGTAGTTATTTGCCGGCGGTATTGAAAGGCTGTCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGTATGGTGTGATTTTGGGACTGATCAACTCTGcc                                                 <  2:221163/250‑1 (MQ=255)                                                                                                                                                                                  aaTATTTGCTGGTGGGGCTTTCATTGGTGCTGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAgg                      >  1:210306/1‑250 (MQ=255)                                                                                                                                                                                           tggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTg                                                   <  1:139624/213‑1 (MQ=255)                                                                                                                                                                                            ggtggGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATAGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGAGGAATTGAAAGGCGGGCGGAACAGCAAGTTGTTTAACCTCGCGGAGTTGGTGCTGGAAGGGGTGATGGGGGGACTGCTCAACGCTGCCGATAGCGAGCTGGTGGTGGGAACCAgggaggtggggg            >  1:138605/1‑240 (MQ=255)                                                                                                                                                                                                gggcgttgaTTGGTGATGTTTTTGGTTCTCTTGCTGGCGCTTGTAGAACATATCGGTTTTACCGGGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGACGTGGGGACTGCTCAACTCTGCCGATAGCGCGCGGTTGTTGGGAACCAGTGTGCTGGTGGTGgc        <  2:100154/243‑1 (MQ=255)                                                                                                                                                                                                gggCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTTTTTCTGGCTGGTGTGGTGTGGGGACTGATCACCTTTGCCGATTGCGCGCTGTTGTTGGGTACCAGGTTGCTGGTTGTGgc        >  2:109539/1‑251 (MQ=255)                                                                                                                                                                                                     ttCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCGGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCATTGTGCTGGTGGTGGCGCTg    <  2:188723/250‑1 (MQ=255)                                                                                                                                                                                                      tCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGGCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGGGGCGCTGGc  >  1:210832/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             CAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGC  >  NC_000913/4638974‑4639421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑