Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,023	A→G	V282V (GTA→GTG)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,023	0	A	G	88.5%	67.1 / NA	26	V282V (GTA→GTG)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (14/9);  minor base T (0/3);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 8.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAAT  >  NC_000913/4638802‑4639265                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   cTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGCAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAc                                                                                                                                                                                                                       >  1:50687/1‑251 (MQ=255)  tttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTCGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAcg                                                                                                                                                                                                                      >  1:117318/1‑250 (MQ=255)                       gTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGc                                                                                                                                                                                                              >  1:198898/1‑238 (MQ=255)                       gTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAAGGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGc                                                                                                                                                                                                              <  2:198898/238‑1 (MQ=255)                                     cccTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAAGCCCAATTAACGGACCGGGCGACTGAGTACGCCATTCTGCCGATTGc                                                                                                                                                                                   <  2:117318/250‑1 (MQ=255)                                                  aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATg                                                                                                                                                                      >  1:116725/1‑251 (MQ=255)                                                         gCATCCCAGTTTTTTAGGTGAAGTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAAGGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTTATCGTTTTGCTTCAGCCAAGGATACCGGCTGGGAAAACTTCCCCGCGGTTGTCGTCGCAGTTACGCCGCCAGCCTATCAGTACCAAGTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGGCtttttttgggggct                                                                                                                                                               <  2:267457/251‑11 (MQ=255)                                                              ccAGTTTTTTAGGCGATTTTTTGCCAGACAAACGGGAACTTAGCGAGTCAGGTTTTCAGGAGCAATGGCAAAGCAGATGGATTGCTAATAATCTCGGTGAGCGTTTTGCTGCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGGCGAGACGACTCCAGCCGATCAATACCAATTTACAGACCCGGCGACTAAGTACCACATTCTACGGATTGCACTCACTTTTATGGCGTGCtttttt                                                                                                                                                          >  2:109785/1‑248 (MQ=255)                                                                                        gCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGttt                                                                                                                                <  1:65453/251‑1 (MQ=255)                                                                                             aCGGGAAGTTAACGAGTCAGGTTTTCAGGCGCAATGGAAAAGCAGCTGTGTTACTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATTCCGGCGGGGAAAACTCCCCCGCGTTTATCGTCGCAGTTACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTTCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTGTGAAACGCTCACCGCGCAACGTTTACAcc                                                                                                                           <  2:109752/251‑1 (MQ=255)                                                                                                   aGTTAGCGAGTCAGGGTTTCAGGCGCAATGGCAAAGCAGCTGGGTTGCTAATAATCGCGGTGAGCGTTTTTCCTCAGGCAATGATACCTGCTGGTAAAACTGCCCCGCGTTGAGCGTCGCAGTTGCGACGCCAGCCGATCAATACCAATTAACTGTCCGTGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTGTGTTTTTGAAACGCTCACCTCGCATCGTGTACACCCAATGc                                                                                                                     <  2:142473/251‑1 (MQ=255)                                                                                                         cGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATAtt                                                                                                               >  1:74083/1‑251 (MQ=255)                                                                                                          gAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATAttt                                                                                                              <  2:74083/251‑1 (MQ=255)                                                                                                              cAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCtg                                                                                                          >  1:188723/1‑251 (MQ=255)                                                                                                               aGGTTTTCAGGAGCAATGGCAAAGCAGCTGGCTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGAAGTGACGACGCCAGCCGCTCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCAATGCCTATTATGGCGTTCTTTGTTTTTGAAACGCCCACCGCGCAACGTTTACACCCAATGCACTCTTTGCtgg                                                                                                         >  2:164379/1‑251 (MQ=255)                                                                                                                                          cTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTCCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGt                                                                                    <  1:192471/245‑1 (MQ=255)                                                                                                                                          cTGGTTTGCTAATAATCTCAGTGAGCGTTTTGCTTCAGGCAATGCTACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTGTGGCGTTATTTGTTTTTGCAACGCTCACCGCGCCACGTTTACACCCAATGCAATATTTTCTGGTGGGGCTTTCATTGGTGATGtcaatcg                                                                              >  2:192471/1‑245 (MQ=255)                                                                                                                                                                        tGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATAAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATatcg                                                >  2:169435/1‑248 (MQ=255)                                                                                                                                                                             cAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGGGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGGGATGGTTTATTTGCTCTTGCTGTCGCTTTCTGAACAGATCGGttt                                            <  2:50687/250‑1 (MQ=255)                                                                                                                                                                                         ccGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGttt                                            >  1:104014/1‑238 (MQ=255)                                                                                                                                                                                         ccGGCTGGGAAAACTTCCCCGCGGTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATGCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCGCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGttt                                            <  2:104014/238‑1 (MQ=255)                                                                                                                                                                                          cGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGCCGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGATGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATgg                               >  2:131685/1‑250 (MQ=255)                                                                                                                                                                                               gggAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTCCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATCTGCTGGTGGGGTTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATc                         >  1:100154/1‑251 (MQ=255)                                                                                                                                                                                                       ttCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGCTCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTTCTGACGCTTTCTGAACATATCGGTTTTACCGAGGCATGGATAATCGCCAGTCt                 >  1:248265/1‑251 (MQ=255)                                                                                                                                                                                                          ccctcGTTTAGCGTCGCAGTGACGCCGCCAGCCGAGCAATACCAATTAACGGACCGGGCGACTACGTACGCCATTCTGCTGATTGCTCTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCTCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCCTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGAt              <  1:109785/247‑1 (MQ=255)                                                                                                                                                                                                                      gTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGTTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAAt  <  1:164379/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   CTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAAT  >  NC_000913/4638802‑4639265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑