Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,927	C→A	H250Q (CAC→CAA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,927	0	C	A	100.0%	64.7 / NA	22	H250Q (CAC→CAA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/12);  total (10/12)

GGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGG  >  NC_000913/4638695‑4639162                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            ggTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGttt                                                                                                                                                                                                                           <  1:135482/251‑1 (MQ=255)    tAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGAACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGGAATAGCGAAATGACCTTAACCAGCCACTGGCCGCATTCCAGTTTTTTAGGGGATTTTTTGCCAGCACAACGGGAAGTTAGCGGGTCAGGTTTTCAGGCGCAATGGCACAACAGCTGGGTTgtt                                                                                                                                                                                                                        >  2:152266/1‑249 (MQ=255)                          gCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGtttt                                                                                                                                                                                                   <  1:244545/250‑1 (MQ=255)                                         cgAAGTGGACTGGCGGAAGCAGAACCGGAAGCTTAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCGGCCGGGCGTAATAGGGAAATGACCTTATCCAGCAACTGGCCGCATCCCAGTTTTGGAGGTGATTTGGTGCCAGCCAAACGGGAAGTTAGCTAGTCAGGTCTTTAGGCGCAAGGGCAATGCATCTGGGGTGGTAATAATCTCGGTGAGCGGTTTgggtcggtcaatgata                                                                                                                                                                                   <  2:111304/250‑16 (MQ=255)                                         cgAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTGTGCTTCAGGCAATGATa                                                                                                                                                                                   <  1:215768/250‑1 (MQ=255)                                                                            atatGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTGGCTAATAATCTCGGTGAGCGTTTTGCTCCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCa                                                                                                                                                <  2:234414/251‑1 (MQ=255)                                                                                ggCCCGGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGGTTAGCGTCGCAGtg                                                                                                                                             >  1:130826/1‑249 (MQ=255)                                                                                                            cTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGCAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAc                                                                                                                >  1:50687/1‑251 (MQ=255)                                                                                                             tttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTCGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAcg                                                                                                               >  1:117318/1‑250 (MQ=255)                                                                                                                                  gTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGc                                                                                                       >  1:198898/1‑238 (MQ=255)                                                                                                                                  gTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAAGGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGc                                                                                                       <  2:198898/238‑1 (MQ=255)                                                                                                                                                cccTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAAGCCCAATTAACGGACCGGGCGACTGAGTACGCCATTCTGCCGATTGc                                                                            <  2:117318/250‑1 (MQ=255)                                                                                                                                                             aCTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATg                                                               >  1:116725/1‑251 (MQ=255)                                                                                                                                                                    gCATCCCAGTTTTTTAGGTGAAGTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAAGGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTTATCGTTTTGCTTCAGCCAAGGATACCGGCTGGGAAAACTTCCCCGCGGTTGTCGTCGCAGTTACGCCGCCAGCCTATCAGTACCAAGTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGGCtttttttgggggct                                                        <  2:267457/251‑11 (MQ=255)                                                                                                                                                                         ccAGTTTTTTAGGCGATTTTTTGCCAGACAAACGGGAACTTAGCGAGTCAGGTTTTCAGGAGCAATGGCAAAGCAGATGGATTGCTAATAATCTCGGTGAGCGTTTTGCTGCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGGCGAGACGACTCCAGCCGATCAATACCAATTTACAGACCCGGCGACTAAGTACCACATTCTACGGATTGCACTCACTTTTATGGCGTGCtttttt                                                   >  2:109785/1‑248 (MQ=255)                                                                                                                                                                                                   gCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGttt                         <  1:65453/251‑1 (MQ=255)                                                                                                                                                                                                        aCGGGAAGTTAACGAGTCAGGTTTTCAGGCGCAATGGAAAAGCAGCTGTGTTACTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATTCCGGCGGGGAAAACTCCCCCGCGTTTATCGTCGCAGTTACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTTCGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTGTGAAACGCTCACCGCGCAACGTTTACAcc                    <  2:109752/251‑1 (MQ=255)                                                                                                                                                                                                              aGTTAGCGAGTCAGGGTTTCAGGCGCAATGGCAAAGCAGCTGGGTTGCTAATAATCGCGGTGAGCGTTTTTCCTCAGGCAATGATACCTGCTGGTAAAACTGCCCCGCGTTGAGCGTCGCAGTTGCGACGCCAGCCGATCAATACCAATTAACTGTCCGTGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTGTGTTTTTGAAACGCTCACCTCGCATCGTGTACACCCAATGc              <  2:142473/251‑1 (MQ=255)                                                                                                                                                                                                                    cGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATAtt        >  1:74083/1‑251 (MQ=255)                                                                                                                                                                                                                     gAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATAttt       <  2:74083/251‑1 (MQ=255)                                                                                                                                                                                                                         cAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCtg   >  1:188723/1‑251 (MQ=255)                                                                                                                                                                                                                          aGGTTTTCAGGAGCAATGGCAAAGCAGCTGGCTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGAAGTGACGACGCCAGCCGCTCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCAATGCCTATTATGGCGTTCTTTGTTTTTGAAACGCCCACCGCGCAACGTTTACACCCAATGCACTCTTTGCtgg  >  2:164379/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            GGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGG  >  NC_000913/4638695‑4639162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑