Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,818	G→C	G214A (GGC→GCC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,818	0	G	C	100.0%	59.3 / NA	20	G214A (GGC→GCC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/12);  total (8/12)

CAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACT  >  NC_000913/4638578‑4639053                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            cAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCTTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAATGGGACTGGCGGAAGCAGAACCTGGAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTTTTTGGTGCCTGCCGGGCGTact                                                                                                                                                                                                                                   >  2:65453/1‑249 (MQ=255)        caggtgaggcaagagctttATTGTGAGGAGGGGCGGGGATGCGCGTGGTAGTGGTGTGGTGAAAGCGCCTGAAGGTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGAAGGGAGAGCATATCCCTGGACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATAGGGACCTGAATTTAAGCGGTACAGGCGATCATTCTGTGGTGCCTGCCGGGCGGAATAGCGaaa                                                                                                                                                                                                                            <  2:72179/235‑1 (MQ=255)                          tATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGTTGTGGTGAAAGCGCCTTAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAAc                                                                                                                                                                                                           <  1:116803/250‑1 (MQ=255)                                                                 ggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGTGTAGAGCAAGGCGTGCAGGGCGTGAATATCCCTATACCCGAAGGGGACTGGCGGAAGCAGAACATGAAGCTGAAGATGGCCCTGAAGTTAAGCGGTACCGGCGATCTTTCGGTGGTGCCTGCCGGGCGTTATAGTGAAATGTCTTTATCCCGCAACTGGTCGCATCCCAGTTTTTTAGGTGAtttgttgcaagcta                                                                                                                                                                   <  2:186689/251‑12 (MQ=255)                                                                     aaaGCGCCTGAAGTTAACGGAACGGCGCTGTCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACg                                                                                                                                                               <  1:167075/251‑1 (MQ=255)                                                                                         aacggCGCTGACCATTGAACCCGGCAGCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGtt                                                                                                                                           <  1:120191/251‑1 (MQ=255)                                                                                            ggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGCAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCCTCCCAGTTTTTTAGGGGATTTTTTGCCAGCCAAACGGGAAGTTAGCGCGTAAGGTTTTc                                                                                                                                        >  1:234414/1‑251 (MQ=255)                                                                                                                      ggTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGttt                                                                                                              <  1:135482/251‑1 (MQ=255)                                                                                                                         tAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGAACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGGAATAGCGAAATGACCTTAACCAGCCACTGGCCGCATTCCAGTTTTTTAGGGGATTTTTTGCCAGCACAACGGGAAGTTAGCGGGTCAGGTTTTCAGGCGCAATGGCACAACAGCTGGGTTgtt                                                                                                           >  2:152266/1‑249 (MQ=255)                                                                                                                                               gCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGtttt                                                                                      <  1:244545/250‑1 (MQ=255)                                                                                                                                                              cgAAGTGGACTGGCGGAAGCAGAACCGGAAGCTTAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCGGCCGGGCGTAATAGGGAAATGACCTTATCCAGCAACTGGCCGCATCCCAGTTTTGGAGGTGATTTGGTGCCAGCCAAACGGGAAGTTAGCTAGTCAGGTCTTTAGGCGCAAGGGCAATGCATCTGGGGTGGTAATAATCTCGGTGAGCGGTTTgggtcggtcaatgata                                                                      <  2:111304/250‑16 (MQ=255)                                                                                                                                                              cgAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTGTGCTTCAGGCAATGATa                                                                      <  1:215768/250‑1 (MQ=255)                                                                                                                                                                                    aaCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCa                                                                                                                                               >  1:130509/1‑156 (MQ=255)                                                                                                                                                                                    aaCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTATCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCa                                                                                                                                               <  2:130509/156‑1 (MQ=255)                                                                                                                                                                                                 atatGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTGGCTAATAATCTCGGTGAGCGTTTTGCTCCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCa                                   <  2:234414/251‑1 (MQ=255)                                                                                                                                                                                                     ggCCCGGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGGTTAGCGTCGCAGtg                                >  1:130826/1‑249 (MQ=255)                                                                                                                                                                                                                                 cTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGCAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAc   >  1:50687/1‑251 (MQ=255)                                                                                                                                                                                                                                  tttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTCGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAAcg  >  1:117318/1‑250 (MQ=255)                                                                                                                                                                                                                                    tCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAACCGGGAAGTTAGCGAGTCAGGttt                                                                                                                                          >  2:218367/1‑113 (MQ=255)                                                                                                                                                                                                                                    tCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGttt                                                                                                                                          <  1:218367/113‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            CAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACT  >  NC_000913/4638578‑4639053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑