Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,939	G→A	F7F (TTC→TTT)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,939	0	G	A	100.0%	76.6 / NA	26	F7F (TTC→TTT)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

AGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGT  >  NC_000913/7711‑8176                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              tgCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTAGTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgcatg                                                                                                                                                                                                                          <  1:174671/249‑1 (MQ=255)          aaCGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCctc                                                                                                                                                                                                                >  1:199980/1‑251 (MQ=255)          aaCGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCctc                                                                                                                                                                                                                >  1:66866/1‑251 (MQ=255)                     ggTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGAGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCAGGCATATCCCTCTTATTGCCGGt                                                                                                                                                                                                     <  1:200584/251‑1 (MQ=255)                                       ccccataacgACTGGTACACAATGCCTGAAAGGAGGTGAAACCGCCGGGCTGTGGATGAAAGCTATTTTTAAGTGTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAAGCCGGTGCGAAAAGTGAACCTACAACCTGCGCCGAAGAGCAGGGAAATCATTCCCGATCCCAAAAGGACGCTGTGAATAAAGGAGAAAAAATCTGGCATGCATATCTCTCTTATTGCCGGTCGCAATGATGTTCCtgtg                                                                                                                                                                                   <  2:258562/242‑1 (MQ=255)                                                    ggTAAACAATGACTGAAATGAGGTTAAACGGGCTGGCTGGGGATGAATGCTATTTTTAAGACGTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCGCTTATTGCCGGTCGCGATGACTTTCCTGTGGAAACGTTAcc                                                                                                                                                                        <  2:164664/249‑1 (MQ=255)                                                     gtaaacaATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAATCGTTACCAAt                                                                                                                                                                     <  2:130570/247‑1 (MQ=255)                                                             tGACTGAAATGAGGTTAACCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGGAGCGAAACTGGACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCAGAAGAGCAGGACAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGGTACCAATTGTTTa                                                                                                                                                               >  2:152345/1‑249 (MQ=255)                                                                            tAAACCGCCTGGCTGTGGATGCATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgt                                                                                                                                                                                    >  2:14221/1‑213 (MQ=255)                                                                            tAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCtgt                                                                                                                                                                                    <  1:14221/213‑1 (MQ=255)                                                                             aacccgccgGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAAGCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGGCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTAc                                                                                                                                              <  2:91720/247‑1 (MQ=255)                                                                               aCCGCCTGGCTGTGGATGAATGCTCTTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGGAACTGCACAAATCCGGTGAGAAAAGTGACCCCACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCCATTGTTTAAGAAGTATATACGCTACg                                                                                                                                             >  2:199519/1‑249 (MQ=255)                                                                                 cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt                                                                                                                                         >  1:19580/1‑251 (MQ=255)                                                                                 cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCCGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt                                                                                                                                         <  1:11303/251‑1 (MQ=255)                                                                                  gCCTGGCTGTGGATGAATGCTATTTTTACGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCCGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGtc                                                                                                                                        >  2:253779/1‑250 (MQ=255)                                                                                           tgGATGAATGCTATTTTTAAGACTTTTGCCACACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAAc                                                                                                                               >  1:213070/1‑251 (MQ=255)                                                                                                gAATGCTATTTTCAAGACTTTTGCCAAACTGGCGGAAGTAGCGACACTGCACAAATCCGGTGCGAAACGTGAACCAACACCCTGCGCAGAAGAGCAGGTACATCATTACCGCTCCCCAACGGACGCTGTTAATAAAGGCGAACAACTCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTAATTGATAACTTCTg                                                                                                                          >  1:32721/1‑251 (MQ=255)                                                                                                                         aaaCTGGCGGATGTAGCGAAACTGCACCAATCCGGTGCGAAAAGAGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAg                                                                                                                                           >  2:90910/1‑209 (MQ=255)                                                                                                                         aaaCTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCGGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGGGTAAACGTTACCAATTGTTGAAGAAGTATATACGCTACGAg                                                                                                                                           <  1:90910/209‑1 (MQ=255)                                                                                                                                              cTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGAACCCCACAAGACCCTGTTACTAAAGGAGCAAAAATCTGCCATGCATATACCTCTTATTGCAGGTCGCGATGACTTTCCTGTGTAAACGTTACTAATTGTTTAAGAAGTATATACGCTACGAGGTACTGGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAACTggcgggcagcatcacc                                                                            >  1:252572/1‑242 (MQ=255)                                                                                                                                                                        aCCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATAGCCCTCTTATGGCCGGTTGCGAGGACTTTCCTGGGTAAACGTTACCAATGGTGTAAGAAGTATATACGCTACGAGGTACTTGATAACTCCTGCGTAGCATATATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAACCAGtct                                                  <  2:67944/251‑1 (MQ=255)                                                                                                                                                                                     gcCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATa                                      >  1:27487/1‑250 (MQ=255)                                                                                                                                                                                                ggTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTcg                          <  1:139789/251‑1 (MQ=255)                                                                                                                                                                                                      tCATTACCGCTCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCATGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATACATTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACACTACCGCCTGGAGATAACCGTATTGTCGGCGGtt                    >  2:89810/1‑251 (MQ=255)                                                                                                                                                                                                               aTCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTTCGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTcggcgggtgggcggg             >  2:215486/1‑240 (MQ=255)                                                                                                                                                                                                                        ggACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGGGTAAACGTTACCAATTGTTTAAGAAGTATAGACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGcgtcgt  <  1:112117/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              AGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGCGTAGCATACATGAGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGT  >  NC_000913/7711‑8176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑