Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,212	G→C	S12T (AGC→ACC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,212	0	G	C	100.0%	90.6 / NA	29	S12T (AGC→ACC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/16);  total (13/16)

CAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGT  >  NC_000913/4637981‑4638448                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             cAAATGGGCAACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTg                                                                                                                                                                                                                           >  1:193601/1‑251 (MQ=255)         cAAAAAAGCAACGGTCTGGGGTTGGCGTGCGTCAGTGAGTTCGCCCGTTTGTGTAACGGCGAAGACACGCGGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGTCTTCACCGTCACTTCACATAGCTGCAAATTCTTCCCACATAGCCTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCtgttggt                                                                                                                                                                                                                   <  2:201038/251‑3 (MQ=255)                 cagcGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGa                                                                                                                                                                                                           <  1:241867/251‑1 (MQ=255)                       tCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTACAGGAAGGCGACGTGCTGGCATCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                                                                                                     >  1:180330/1‑251 (MQ=255)                         tGGGGCTGGAGTTCGTCAGTGAGGACCCCCGTTTGTTTCACGGCGCAGTAACGCTGCGCAACGTGCAGGCCGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATCGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATActgctg                                                                                                                                                                                                   >  2:251785/1‑251 (MQ=255)                                ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGc                                                                                                                                                                                            <  1:82803/251‑1 (MQ=255)                                   gTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCa                                                                                                                                                                                           >  1:64695/1‑249 (MQ=255)                                       gTCAGTGAGGTCGCCCGTTTGTGTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCAGTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGGTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGAt                                                                                                                                                                                     <  2:133303/251‑1 (MQ=255)                                            tGAGGTCGCCCGTTTGTTTAACGGCGAAGTCCCGCTACGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAACTTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGAAAGTGATTGTCg                                                                                                                                                                                >  2:171483/1‑251 (MQ=255)                                              aGGTCGACCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTGCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACGATGTTGAAATCCCCCCTGTTCTTGAAAATGTCCACCTTGGTTGGTTCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCTGCAAGTGATTGTCGaa                                                                                                                                                                              <  1:13050/251‑1 (MQ=255)                                               ggTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAAc                                                                                                                                                                             <  1:6031/251‑1 (MQ=255)                                                  cGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACGTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTTTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTg                                                                                                                                                                          <  2:85888/251‑1 (MQ=255)                                                     ccGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGccg                                                                                                                                                                       <  1:133478/251‑3 (MQ=255)                                                                          cACGCTGCTCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAg                                                                                                                                                  <  1:104867/251‑1 (MQ=255)                                                                                 cgcAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAATTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTATGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGGGATTGTCGAACGTGCCGATTAACGTAGCGATGTGGAAGATGCGAt                                                                                                                                           >  2:167075/1‑251 (MQ=255)                                                                                         gCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCaa                                                                                                                                    >  1:127481/1‑250 (MQ=255)                                                                                                        gCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCg                                                                                                                     <  2:112121/250‑1 (MQ=255)                                                                                                                      gACTTCACCGTCCCTTCACATAGCTTCAAATTATTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGCGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                                                                                                     >  2:191846/1‑156 (MQ=255)                                                                                                                      gACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                                                                                                     <  1:191846/156‑1 (MQ=255)                                                                                                                              cGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGAGTCGCTAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCttat                                                                                              <  2:21403/251‑4 (MQ=255)                                                                                                                                     tCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTa                                                                                         <  1:247743/249‑1 (MQ=255)                                                                                                                                        caTAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCt                                                                                    <  1:176622/251‑1 (MQ=255)                                                                                                                                                                     tCGTATCCTGCTGCCATTGCAAATGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGAGTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGTTGCAGGaa                                                         <  2:193601/249‑1 (MQ=255)                                                                                                                                                                          tCCTGCTGCCATTGCAAAGGAGAAGACTATGATGAAATCCCCCCTGTTCCGGAAAATGACCACCTTGTTTGGTGAAGTATTGCTGTTGTTGATTCCGGTAATGATGCTTCGGCAAAGGATTGTCGAAGGTGCAGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGATGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATAAGATGCAGGAAGAGGAt                                                   >  1:244464/1‑250 (MQ=255)                                                                                                                                                                             tgctgcAATTGCAAAGGAGAAGACTATGTTGACATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGCTAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTTGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCCAAAACTCGTTGTGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaccc                                               >  1:72179/1‑248 (MQ=255)                                                                                                                                                                                 gcCATTGCAAAGTAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGGACGTGCCGATTACCGTAGCGATGTGGAAGATGCGGTTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATCCGGTGCAGGAGGAGGTTAACACCgg                                           >  2:78373/1‑250 (MQ=255)                                                                                                                                                                                                                ccccccTGTTCTGGAAAATGACCTCCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTg             <  1:185522/250‑1 (MQ=255)                                                                                                                                                                                                                     cTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTAcc       >  1:82029/1‑251 (MQ=255)                                                                                                                                                                                                                          cTGGAAAATGACCACCTTGTTTGGTGCAGTCTTGATGTTGTTGATTCCGATCATGCTGATTCGGCAAGTGATTGTCGCACGTGCCGATTCCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACAAGCGGGCCGCAAAAACTCGTTGGGCCGCTTCTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGt  >  2:246886/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             CAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGT  >  NC_000913/4637981‑4638448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑