Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,142	T→C	intergenic (+22/‑36)	creC → / → creD	sensory histidine kinase CreC/putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,142	0	T	C	100.0%	112.0 / NA	35	intergenic (+22/‑36)	creC/creD	sensory histidine kinase CreC/putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

AGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCT  >  NC_000913/4637903‑4638366                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 cggTGCTGGATACCGGTAGTGGTATTCCTGACTTCGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCATGGATGTCGCCCTTTTGTTTAGCGGCGAAGTCTCGCTGCGCAACGTGCAGGAAGGTTGCGTTCTGGCCTCGCTTCGACTTCACTGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCct                                                                                                                                                                                                                       <  1:43739/250‑1 (MQ=255)          aTACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCAtt                                                                                                                                                                                                              <  1:72734/251‑1 (MQ=255)                 tAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGAGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGCAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAgg                                                                                                                                                                                                       >  2:176622/1‑251 (MQ=255)                  aGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAggg                                                                                                                                                                                                      >  1:13467/1‑250 (MQ=255)                             tGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGt                                                                                                                                                                                           >  2:213367/1‑251 (MQ=255)                              gACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTGTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGGCACTTCACATAGCTTCAAATTCTGCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATg                                                                                                                                                                                            <  1:223959/249‑1 (MQ=255)                                                        aCGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGa                                                                                                                                                                <  1:159855/251‑1 (MQ=255)                                                                    tttGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGAccaccttgtttg                                                                                                                                                    >  1:198728/1‑240 (MQ=255)                                                                        ccgcGTGCAAATGGGCAAAAAAGCAGCGGGCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGTGAAGTCACGCTGCGCAACGTGCAGGAAGGGGGCGGGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAAGTACTCCCACATATCCTTCGTATCCTGCTGCCATTTCAAAGGAGAAGACTATGTTGAAATCCCCACTGCTCTGGAAAATGAccaccttggttggtgc                                                                                                                                                <  2:269104/248‑16 (MQ=255)                                                                             tGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGagaa                                                                                                                                                                                                   >  1:83370/1‑195 (MQ=255)                                                                             tGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTACCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGagaa                                                                                                                                                                                                   <  2:83370/195‑1 (MQ=255)                                                                               cAAATGGGCAACAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTg                                                                                                                                         >  1:193601/1‑251 (MQ=255)                                                                                       cAAAAAAGCAACGGTCTGGGGTTGGCGTGCGTCAGTGAGTTCGCCCGTTTGTGTAACGGCGAAGACACGCGGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGTCTTCACCGTCACTTCACATAGCTGCAAATTCTTCCCACATAGCCTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCtgttggt                                                                                                                                 <  2:201038/251‑3 (MQ=255)                                                                                               cagcGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTGCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGa                                                                                                                         <  1:241867/251‑1 (MQ=255)                                                                                                     tCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTACAGGAAGGCGACGTGCTGGCATCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                   >  1:180330/1‑251 (MQ=255)                                                                                                       tGGGGCTGGAGTTCGTCAGTGAGGACCCCCGTTTGTTTCACGGCGCAGTAACGCTGCGCAACGTGCAGGCCGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATCGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATActgctg                                                                                                                 >  2:251785/1‑251 (MQ=255)                                                                                                              ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGc                                                                                                          <  1:82803/251‑1 (MQ=255)                                                                                                                 gTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCa                                                                                                         >  1:64695/1‑249 (MQ=255)                                                                                                                     gTCAGTGAGGTCGCCCGTTTGTGTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCAGTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGGTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGAt                                                                                                   <  2:133303/251‑1 (MQ=255)                                                                                                                          tGAGGTCGCCCGTTTGTTTAACGGCGAAGTCCCGCTACGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAACTTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGAAAGTGATTGTCg                                                                                              >  2:171483/1‑251 (MQ=255)                                                                                                                            aGGTCGACCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTGCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACGATGTTGAAATCCCCCCTGTTCTTGAAAATGTCCACCTTGGTTGGTTCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCTGCAAGTGATTGTCGaa                                                                                            <  1:13050/251‑1 (MQ=255)                                                                                                                             ggTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAAc                                                                                           <  1:6031/251‑1 (MQ=255)                                                                                                                                cGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACGTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTTTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTg                                                                                        <  2:85888/251‑1 (MQ=255)                                                                                                                                   ccGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGccg                                                                                     <  1:133478/251‑3 (MQ=255)                                                                                                                                                        cACGCTGCTCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAg                                                                <  1:104867/251‑1 (MQ=255)                                                                                                                                                        cACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCTCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctg                                                                                                                                                                                                                      <  2:69883/101‑1 (MQ=255)                                                                                                                                                        cACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCctg                                                                                                                                                                                                                      >  1:69883/1‑101 (MQ=255)                                                                                                                                                               cgcAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGAATTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTATGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGGGATTGTCGAACGTGCCGATTAACGTAGCGATGTGGAAGATGCGAt                                                         >  2:167075/1‑251 (MQ=255)                                                                                                                                                                       gCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCaa                                                  >  1:127481/1‑250 (MQ=255)                                                                                                                                                                                      gCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCg                                   <  2:112121/250‑1 (MQ=255)                                                                                                                                                                                                    gACTTCACCGTCCCTTCACATAGCTTCAAATTATTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGCGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                   >  2:191846/1‑156 (MQ=255)                                                                                                                                                                                                    gACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                                                                                   <  1:191846/156‑1 (MQ=255)                                                                                                                                                                                                            cGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGAGTCGCTAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCttat            <  2:21403/251‑4 (MQ=255)                                                                                                                                                                                                                   tCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTa       <  1:247743/249‑1 (MQ=255)                                                                                                                                                                                                                      caTAGCTTCAAATTCTTCCCACATAGCCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACCACCTTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAAGTGATTGTCGAACGTGCCGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCt  <  1:176622/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 AGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCT  >  NC_000913/4637903‑4638366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑