Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,862	C→A	I389I (ATC→ATA)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,862	0	C	A	100.0%	72.0 / NA	25	I389I (ATC→ATA)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/14);  total (11/14)

GTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCG  >  NC_000913/4637617‑4638107                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gTAGAAACGTTACTGCGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTAGTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCGCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGATCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCt                                                                                                                                                                                                                                                  <  1:145956/251‑1 (MQ=255)      aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTATTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgcg                                                                                                                                                                                                                                             >  1:201038/1‑251 (MQ=255)                  ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGCGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATc                                                                                                                                                                                                                                 >  1:133303/1‑251 (MQ=255)                        aaGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCCCTGAGGGTAATGTTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGGTAACGCCATCGGTTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAac                                                                                                                                                                                                                           >  2:82673/1‑251 (MQ=255)                              ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCCTCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGGAGTGGATCAGGAACACGTcg                                                                                                                                                                                                                     >  2:65310/1‑250 (MQ=255)                                     cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAacacttcgccctta                                                                                                                                                                                                               <  2:264286/250‑11 (MQ=255)                                              gTCGTTCTGACTGTTGTTGATGTGGCGGCATTTTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCGGTTGGCAGAAAAAAACATCACTCTAAATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTACGCGCCGAAGTGGATCAGGAACCCGTCGCACTTAAGGGGCTggg                                                                                                                                                                                                     >  2:82803/1‑250 (MQ=255)                                                            tgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGt                                                                                                                                                                                        <  1:82673/250‑1 (MQ=255)                                                                                  cgccgcGTCAGCGAAGCGCGCACCGTGCAGTTTGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTCAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCtggcacg                                                                                                                                                                 >  2:72734/1‑245 (MQ=255)                                                                                          cAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAttttt                                                                                                                                                          <  2:76439/250‑1 (MQ=255)                                                                                                         cGTGCAGTTGGCAGAAAAACACCTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGACGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGGATTCCTGACTACGCGCTGTAGCGTAGTTTTGAACGCttttcatctt                                                                                                                                          >  2:247743/1‑245 (MQ=255)                                                                                                                  ggCAGAACAAAACATCTCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGATAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCCCGTATTTTTGAACGCTTTTACTCTTTGCCGCGTg                                                                                                                                 >  2:133478/1‑251 (MQ=255)                                                                                                                      gAAAAAAACATCACTCTACATGGTATTCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCaaa                                                                                                                             <  2:234935/251‑1 (MQ=255)                                                                                                                              caTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAGTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaa                                                                                                                     <  2:135212/250‑1 (MQ=255)                                                                                                                                    tctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagc                                                                                                                >  1:185190/5‑250 (MQ=255)                                                                                                                                           gTTAAGCCCACTGGGGTTAAAGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGGAATTTACTGGAATACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCt                                                                                                        <  2:93332/251‑1 (MQ=255)                                                                                                                                                gCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                                                                    <  1:232578/250‑1 (MQ=255)                                                                                                                                                    cctgAGGTTAATGGTGCTGCGGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATCTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGc                                                                                               <  2:107881/248‑1 (MQ=255)                                                                                                                                                       gAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAACAAGCAGCGGTCTGGGGTCGGCGtt                                                                                            >  2:6031/1‑251 (MQ=255)                                                                                                                                                        aGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc                                                                                           >  1:112121/1‑251 (MQ=255)                                                                                                                                                                            ccGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgttt                                                                       <  1:65310/251‑1 (MQ=255)                                                                                                                                                                                              gcgcTGGGGAAGTTACTGGATAACGCCACCGATTTTACCCCCGCGGGCGGTCGCATAACGATAAGCGCCGAAGTGGATCAGGAACACGTCGCACGTAAGTTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGGTCGTCAGTGAGGGCGCCCGTTTGTTTAACGGCTATGTCAc                                                     <  2:238148/251‑1 (MQ=255)                                                                                                                                                                                                        aTTTACTGGATAACGCCATCGAGTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAAc                                           <  1:249301/251‑1 (MQ=255)                                                                                                                                                                                                                 aTAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACTCGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTGTGTGTAACGGCGAAGTCACGCTGCGCAACGTGCAGGaa                                  <  2:49473/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cgCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCg  <  1:137032/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCG  >  NC_000913/4637617‑4638107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑