Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,814	A→G	G373G (GGA→GGG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,814	0	A	G	100.0%	104.2 / NA	34	G373G (GGA→GGG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

CGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCAC  >  NC_000913/4637569‑4638056                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   cGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTAGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAtt                                                                                                                                                                                                                                                <  1:239517/250‑1 (MQ=255)        ccgACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGAt                                                                                                                                                                                                                                        <  1:254157/249‑1 (MQ=255)            caacaGTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACg                                                                                                                                                                                                                                    <  1:198444/251‑1 (MQ=255)                aTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCAGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCAt                                                                                                                                                                                                                                >  1:21403/1‑251 (MQ=255)                  tCTGACGCAAAATGCGCGTATGAAGGCATTGGTAGAAACGTTACTACGCCAGGCAATACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTTTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCTCTGAGGTTAATGTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTGGGGAATTTCCTGGATAACTCCATc                                                                                                                                                                                                                               <  2:149215/250‑1 (MQ=255)                     gACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGAt                                                                                                                                                                                                                            <  2:210598/250‑1 (MQ=255)                         cAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGCGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAc                                                                                                                                                                                                                       >  1:93332/1‑251 (MQ=255)                          aaaaTGCGCGTATGCAGGCCTTGGTAGCAACGTTACTACGCCAGGCAGGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGACGTGGCGGCCTTATTTCGCCGGGTCAGCGAAGCGCGCACCGGGCCGTTGGCAGCAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTCATGATGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGctcccgtttttacc                                                                                                                                                                                                                      >  2:223959/1‑238 (MQ=255)                          aaaaTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCCGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGCAGCGCGCACCGTGCCGGTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGGTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTTGATACCGCCATCGCTTTTAcc                                                                                                                                                                                                                      >  2:241867/1‑251 (MQ=255)                           aaaTGCGCGTATGCAGGCATTGGTAGAAACGCTACTACGCCAGCAAAGACTGGCGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGCGGCGGCATTATTTCGCCCCGTAAGCGAAGCACGCCCCGTGCAGTTGGCAGAAAAAAAAATCAATCTACATGTTATGCCCACTGCGGTTAATGTTGCTGATGAACCGGCGTTCCTGGCGCAGGCGCTGGGGAATTTCCTGGATCCCGCCATCGATTTTAccc                                                                                                                                                                                                                     >  2:137032/1‑251 (MQ=255)                               gcgcGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGTCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCg                                                                                                                                                                                                                  <  2:57102/250‑1 (MQ=255)                                               tGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAtaac                                                                                                                                                                                                  <  1:14362/250‑4 (MQ=255)                                                 gTAGAAACGTTACTGCGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTAGTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCGCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGATCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCt                                                                                                                                                                                               <  1:145956/251‑1 (MQ=255)                                                      aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTATTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgcg                                                                                                                                                                                          >  1:201038/1‑251 (MQ=255)                                                                  ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGCGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATc                                                                                                                                                                              >  1:133303/1‑251 (MQ=255)                                                                        aaGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCCCTGAGGGTAATGTTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGGTAACGCCATCGGTTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAac                                                                                                                                                                        >  2:82673/1‑251 (MQ=255)                                                                              ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCCTCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGGAGTGGATCAGGAACACGTcg                                                                                                                                                                  >  2:65310/1‑250 (MQ=255)                                                                                     cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAacacttcgccctta                                                                                                                                                            <  2:264286/250‑11 (MQ=255)                                                                                              gTCGTTCTGACTGTTGTTGATGTGGCGGCATTTTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCGGTTGGCAGAAAAAAACATCACTCTAAATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTACGCGCCGAAGTGGATCAGGAACCCGTCGCACTTAAGGGGCTggg                                                                                                                                                  >  2:82803/1‑250 (MQ=255)                                                                                                            tgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGt                                                                                                                                     <  1:82673/250‑1 (MQ=255)                                                                                                                                  cgccgcGTCAGCGAAGCGCGCACCGTGCAGTTTGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTCAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCtggcacg                                                                                                              >  2:72734/1‑245 (MQ=255)                                                                                                                                          cAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTAttttt                                                                                                       <  2:76439/250‑1 (MQ=255)                                                                                                                                                         cGTGCAGTTGGCAGAAAAACACCTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGACGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGGATTCCTGACTACGCGCTGTAGCGTAGTTTTGAACGCttttcatctt                                                                                       >  2:247743/1‑245 (MQ=255)                                                                                                                                                                  ggCAGAACAAAACATCTCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGATAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCCCGTATTTTTGAACGCTTTTACTCTTTGCCGCGTg                                                                              >  2:133478/1‑251 (MQ=255)                                                                                                                                                                      gAAAAAAACATCACTCTACATGGTATTCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCaaa                                                                          <  2:234935/251‑1 (MQ=255)                                                                                                                                                                              caTCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAGTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCaaa                                                                  <  2:135212/250‑1 (MQ=255)                                                                                                                                                                                    tctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAagc                                                             >  1:185190/5‑250 (MQ=255)                                                                                                                                                                                           gTTAAGCCCACTGGGGTTAAAGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGGAATTTACTGGAATACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCt                                                     <  2:93332/251‑1 (MQ=255)                                                                                                                                                                                                gCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTgggg                                                 <  1:232578/250‑1 (MQ=255)                                                                                                                                                                                                    cctgAGGTTAATGGTGCTGCGGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATCTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGc                                            <  2:107881/248‑1 (MQ=255)                                                                                                                                                                                                       gAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAACAAGCAGCGGTCTGGGGTCGGCGtt                                         >  2:6031/1‑251 (MQ=255)                                                                                                                                                                                                        aGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTc                                        >  1:112121/1‑251 (MQ=255)                                                                                                                                                                                                                            ccGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCgttt                    <  1:65310/251‑1 (MQ=255)                                                                                                                                                                                                                                              gcgcTGGGGAAGTTACTGGATAACGCCACCGATTTTACCCCCGCGGGCGGTCGCATAACGATAAGCGCCGAAGTGGATCAGGAACACGTCGCACGTAAGTTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGGTCGTCAGTGAGGGCGCCCGTTTGTTTAACGGCTATGTCAc  <  2:238148/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   CGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCAC  >  NC_000913/4637569‑4638056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑