Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,675	C→T	A327V (GCT→GTT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,675	0	C	T	100.0%	123.8 / NA	40	A327V (GCT→GTT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

ATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGA  >  NC_000913/4637431‑4637912                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              aTGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGttgttg                                                                                                                                                                                                                                          >  1:76439/1‑250 (MQ=255)           aGCTGGAAGGGAAACACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggc                                                                                                                                                                                                                                >  1:49473/1‑250 (MQ=255)              tGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTCAAAAGCCCACTGGCGGCGAATCGTGGCGCGGCGGAAATTTTACGCGAAAGGCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGAGCGTATGAAGGCATTGGTAGAAACGTTACTACGCCACGAAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggccttt                                                                                                                                                                                                                            >  1:269104/1‑247 (MQ=255)               ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCatt                                                                                                                                                                                                                             <  1:254356/249‑1 (MQ=255)                 aaGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                                                                                                                                                                                                                         <  1:174931/251‑2 (MQ=255)                  aGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatttc                                                                                                                                                                                                                        <  2:208605/251‑3 (MQ=255)                                 tGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACGACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTTTTGGTGATGTGACGGCATTAGTTCGCCGCGTCAGCGaa                                                                                                                                                                                                          <  2:122595/250‑1 (MQ=255)                                   aGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGGAATTTTACGCGAAAGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAACATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGTTGTTGATGTGGCGGGattttttgccccgggcagcgagggg                                                                                                                                                                                                       >  2:159855/1‑229 (MQ=255)                                     cAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAgcgcg                                                                                                                                                                                                     <  1:52764/251‑1 (MQ=255)                                                 gcgtttacccatgagagaaaaaGCCCACTGGCGTCGATTCGTGGCGCGTCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGAGGTTGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGtt                                                                                                                                                                                         <  2:267396/234‑1 (MQ=255)                                                  cgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTg                                                                                                                                                                                        <  1:224095/249‑1 (MQ=255)                                                         ccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACGGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATGATTTCGCCGCGGCAGCGAAGCGCGCAACGTGCAGGTGGCAGaaa                                                                                                                                                                                 <  2:137208/250‑1 (MQ=255)                                                                       gCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCActcta                                                                                                                                                                   <  1:264671/251‑2 (MQ=255)                                                                            cTGGCAGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACCTTCTGCCGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTTGCAGAAAAAAACATCCCTCTACATGt                                                                                                                                                              >  2:249301/1‑251 (MQ=255)                                                                               gcggcgATTCGTGGCGCGGCGGAACTTTTACGCGCAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGGCAGCGGAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTat                                                                                                                                                           >  2:232578/1‑250 (MQ=255)                                                                                 ggcgATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGAAGAAAAAAACATCACTCTACATGTTatgc                                                                                                                                                         >  1:57102/1‑248 (MQ=255)                                                                                   cgATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTTTTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTTACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGccc                                                                                                                                                       <  1:250708/251‑1 (MQ=255)                                                                                     aTTCGTGGCGCGGCGGAAATTTTACGCGACGGTCCGCCACCGGAAGTGGCGGCTCGTTTTCCCGACAACCTTCTGACGCAAAATGCGCGTATGCAGGGATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGGTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTatggccac                                                                                                                                                     >  1:107881/1‑244 (MQ=255)                                                                                     aTTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCTGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCCTTGGTAGAACCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTTGCGGCATTCTTTCGCCGCGTCAGCGGAGCGCGCACCGTGCAGTTGGCAGAAAACAACATCACTCTACATTTTATGCCCAc                                                                                                                                                     >  1:238148/1‑251 (MQ=255)                                                                                                     aaaTTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTtgct                                                                                                                                     <  1:60691/251‑1 (MQ=255)                                                                                                          ttACGCGAAGGGCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGctgctg                                                                                                                                 <  2:107596/250‑1 (MQ=255)                                                                                                                              ggAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAAACGGCGTTACTGGAGCAgg                                                                                                            >  1:85888/1‑251 (MQ=255)                                                                                                                                           cGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTAGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAtt                                                                                                <  1:239517/250‑1 (MQ=255)                                                                                                                                                  ccgACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGAt                                                                                        <  1:254157/249‑1 (MQ=255)                                                                                                                                                      caacaGTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACg                                                                                    <  1:198444/251‑1 (MQ=255)                                                                                                                                                          aTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCAGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCAt                                                                                >  1:21403/1‑251 (MQ=255)                                                                                                                                                            tCTGACGCAAAATGCGCGTATGAAGGCATTGGTAGAAACGTTACTACGCCAGGCAATACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTTTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCTCTGAGGTTAATGTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTGGGGAATTTCCTGGATAACTCCATc                                                                               <  2:149215/250‑1 (MQ=255)                                                                                                                                                               gACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGAt                                                                            <  2:210598/250‑1 (MQ=255)                                                                                                                                                                   cAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGCGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAc                                                                       >  1:93332/1‑251 (MQ=255)                                                                                                                                                                    aaaaTGCGCGTATGCAGGCCTTGGTAGCAACGTTACTACGCCAGGCAGGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGACGTGGCGGCCTTATTTCGCCGGGTCAGCGAAGCGCGCACCGGGCCGTTGGCAGCAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTCATGATGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGctcccgtttttacc                                                                      >  2:223959/1‑238 (MQ=255)                                                                                                                                                                    aaaaTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCCGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGCAGCGCGCACCGTGCCGGTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGGTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTTGATACCGCCATCGCTTTTAcc                                                                      >  2:241867/1‑251 (MQ=255)                                                                                                                                                                     aaaTGCGCGTATGCAGGCATTGGTAGAAACGCTACTACGCCAGCAAAGACTGGCGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGCGGCGGCATTATTTCGCCCCGTAAGCGAAGCACGCCCCGTGCAGTTGGCAGAAAAAAAAATCAATCTACATGTTATGCCCACTGCGGTTAATGTTGCTGATGAACCGGCGTTCCTGGCGCAGGCGCTGGGGAATTTCCTGGATCCCGCCATCGATTTTAccc                                                                     >  2:137032/1‑251 (MQ=255)                                                                                                                                                                         gcgcGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGTCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCg                                                                  <  2:57102/250‑1 (MQ=255)                                                                                                                                                                                         tGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCAtaac                                                  <  1:14362/250‑4 (MQ=255)                                                                                                                                                                                           gTAGAAACGTTACTGCGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTAGTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCGCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGATCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCt                                               <  1:145956/251‑1 (MQ=255)                                                                                                                                                                                                aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCCCCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTATTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgcg                                          >  1:201038/1‑251 (MQ=255)                                                                                                                                                                                                            ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGCGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATc                              >  1:133303/1‑251 (MQ=255)                                                                                                                                                                                                                  aaGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCCCTGAGGGTAATGTTGATGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGGTAACGCCATCGGTTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAac                        >  2:82673/1‑251 (MQ=255)                                                                                                                                                                                                                        ggAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGCTAACGCCCTCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGGAGTGGATCAGGAACACGTcg                  >  2:65310/1‑250 (MQ=255)                                                                                                                                                                                                                               cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAacacttcgccctta            <  2:264286/250‑11 (MQ=255)                                                                                                                                                                                                                                        gTCGTTCTGACTGTTGTTGATGTGGCGGCATTTTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCGGTTGGCAGAAAAAAACATCACTCTAAATGTTATGCCCACTGGGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTACGCGCCGAAGTGGATCAGGAACCCGTCGCACTTAAGGGGCTggg  >  2:82803/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGA  >  NC_000913/4637431‑4637912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑