Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,478	C→T	Y261Y (TAC→TAT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,478	0	C	T	100.0%	101.6 / NA	33	Y261Y (TAC→TAT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

GGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCG  >  NC_000913/4637240‑4637717                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                ggcgAATTTTATGGGCCAGCGCCATTTTGCTGGTGCTTTAACTGATGCTTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACACGGCAGTAGCGAGTTGAGTAAGCTTGCGCAGGCGCTGGAAAGAATGCGCGTGAAGCTGGAAGGGAAAAAATATATTGAGCAGTATGTTTATGCGTTAACCCAt                                                                                                                                                                                                                                     >  2:14362/1‑251 (MQ=255)          tATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGCTTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTaaa                                                                                                                                                                                                                             >  2:52764/1‑250 (MQ=255)                               tgGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCgc                                                                                                                                                                                                       >  2:254157/1‑250 (MQ=255)                                 gggATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTgg                                                                                                                                                                                                     >  2:224095/1‑251 (MQ=255)                                         aCTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGcggcgg                                                                                                                                                                                             >  1:234935/1‑251 (MQ=255)                                                              ggTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTccg                                                                                                                                                                         <  2:73579/250‑1 (MQ=255)                                                                                  ccaTTGCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCg                                                                                                                                                     <  1:214686/248‑1 (MQ=255)                                                                                  ccaTTGCCCGGCTCACTCGCTATGCCGAGTCCGTCACTGACAATAAGCCCGATCCTCTTCCGGAACTCGGCAGTAGCGAGTTGCGTAAGCGTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGGTTGTTGATGCGTTAACCCATGAGCTAAAAAGGCCACTGGCGGCGAGTCGTGTCGCGGCGGAAATGTTACGCGAAGGTCCGCCACCGGAAGTGTTGGCTc                                                                                                                                                      <  2:108669/247‑1 (MQ=255)                                                                                       gCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTa                                                                                                                                                <  1:125039/250‑1 (MQ=255)                                                                                       gCCCGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGGGGTGGCTCGTTTTAc                                                                                                                                               >  1:264286/1‑251 (MQ=255)                                                                                         ccGGCTCACTCGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTAccg                                                                                                                                             >  2:60691/1‑249 (MQ=255)                                                                                          cGGCTCACTCGCTATGCCGATTCCGTCACTGACACTAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGCTGGCTCGTTTTAccgc                                                                                                                                            >  2:239517/1‑248 (MQ=255)                                                                                                   cGCTATGCCGATTCCGTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCt                                                                                                                                   >  1:135212/1‑251 (MQ=255)                                                                                                                  gTCACTGACAATAAGCCCGTTCCTCTTCCTGAACTCGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGcgcg                                                                                                                    <  2:163228/251‑1 (MQ=255)                                                                                                                                                gaactcGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTtacta                                                                                        <  1:140776/246‑1 (MQ=255)                                                                                                                                                 aactcGGCAGTAGCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGc                                                                                     >  1:137208/3‑251 (MQ=255)                                                                                                                                                     cggcagtagCGAGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCGGGAAGGGAAAAACAATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCGAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCGCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAgg                                                                                 <  2:2193/247‑1 (MQ=255)                                                                                                                                                                aGTTGCGTAAGCTTGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCGGGAAGGGAAAAACTATATTGAGCAGTATGGTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGGTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTTCTCCACGCAAGGCTGgag                                                                      <  2:120553/251‑1 (MQ=255)                                                                                                                                                                              gcgcATGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTAGATTTAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGTCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCGCGTTTTACCGACAACATTCTGTCGCAAAATGCGCGTAGGCATGTATTGGTAGAAACGTTACTACGCCAGGcgtggcgggttaatcgttaggaag                                                         <  2:52499/250‑24 (MQ=255)                                                                                                                                                                               cgcAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATGCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTc                                                       <  1:39214/251‑1 (MQ=255)                                                                                                                                                                                  aGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGtt                                                    >  1:107596/1‑251 (MQ=255)                                                                                                                                                                                    gcgcTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCt                                                  <  2:219291/251‑1 (MQ=255)                                                                                                                                                                                      gcTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGa                                                <  1:245048/251‑1 (MQ=255)                                                                                                                                                                                         ggAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCAGTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGAc                                               <  2:82470/249‑1 (MQ=255)                                                                                                                                                                                                aTGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGttgttg                                       >  1:76439/1‑250 (MQ=255)                                                                                                                                                                                                          aGCTGGAAGGGAAACACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggc                             >  1:49473/1‑250 (MQ=255)                                                                                                                                                                                                             tGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTCAAAAGCCCACTGGCGGCGAATCGTGGCGCGGCGGAAATTTTACGCGAAAGGCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGAGCGTATGAAGGCATTGGTAGAAACGTTACTACGCCACGAAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTggcggccttt                         >  1:269104/1‑247 (MQ=255)                                                                                                                                                                                                              ggAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCatt                          <  1:254356/249‑1 (MQ=255)                                                                                                                                                                                                                aaGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattattt                      <  1:174931/251‑2 (MQ=255)                                                                                                                                                                                                                 aGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatttc                     <  2:208605/251‑3 (MQ=255)                                                                                                                                                                                                                                tGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACGACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTTTTGGTGATGTGACGGCATTAGTTCGCCGCGTCAGCGaa       <  2:122595/250‑1 (MQ=255)                                                                                                                                                                                                                                  aGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGGAATTTTACGCGAAAGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAACATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGTTGTTGATGTGGCGGGattttttgccccgggcagcgagggg    >  2:159855/1‑229 (MQ=255)                                                                                                                                                                                                                                    cAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAgcgcg  <  1:52764/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                GGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCG  >  NC_000913/4637240‑4637717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑