Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,627	A→G	S111S (TCT→TCC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,627	0	A	G	100.0%	40.5 / NA	16	S111S (TCT→TCC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

TGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT  >  NC_000913/7384‑7842                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        cgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATAAAAAGAAAAACTCAGGGCGCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCTAGGGAACAt                                                                                                                                                                                                                   <  2:38647/249‑1 (MQ=255)       cagAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCAAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCa                                                                                                                                                                                                             >  2:200584/1‑250 (MQ=255)                            aagaccagTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCTCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATaa                                                                                                                                                                                       <  2:212845/244‑1 (MQ=255)                                              gggAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAACTCCGCGGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCAGCCCGCGCGCCAGATACCATGCCGGGCCGCCACGAAAGTGCCCATTGGCATCACGTTCTTCATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATcc                                                                                                                                                                     <  1:162493/251‑1 (MQ=255)                                                     ataaaaaaaaaacaaaggggccgctcAACGTCGTTCGCCGGAATTCCGCTGAAAATTATGCCGTAGGACAGGAGCAAAAAAACGGCGAAAAAAATGCCGAACCAGCGAATTCCCAGCCCGCGCGCCATACACCATGCCGTTCCGCCACGAAACTGGCCAGTGGCATCACGTTCAGTAAAAAGCTGTGCGAGGGAACATGCGGAAAAAGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGAc                                                                                                                                                              <  1:134339/226‑1 (MQ=255)                                                               aaCTCAGGGATCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACTGCTCCAGGt                                                                                                                                                    >  1:60908/1‑251 (MQ=255)                                                                                                         aaaTTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCg                                                                                                          <  1:125507/251‑1 (MQ=255)                                                                                                              aTGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCGCGCAACGCCGGCCCGGTTTCCGCCAcc                                                                                                     >  1:130570/1‑251 (MQ=255)                                                                                                                    tAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCGACCCCcgcg                                                                                               >  1:91720/1‑251 (MQ=255)                                                                                                                      ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCAGGCCGATAAACGCGGCAGCCCACATCCAGAATACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCTCAACGCCGGGCAGGGTGCCTCTACCCAcgcgcg                                                                                             <  2:43140/251‑1 (MQ=255)                                                                                                                       gCGATGAGCAAAAATACGGCGAACAGAACGCCCATCCAGCACATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACgcgcgc                                                                                            <  2:245864/251‑1 (MQ=255)                                                                                                                           tGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCACAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAACGCCACCCCGGCCCGGTTGCCGCCACCCACCCGcgccgcc                                                                                        >  2:174671/1‑250 (MQ=255)                                                                                                                                               aGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCACGCACCGCCGGCCCGGGTGCCCCTCCCCACACGCGCCCCGAGACTggcaccaacagaatg                                                                    >  2:11303/1‑238 (MQ=255)                                                                                                                                                 aactcccATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGaa                                                                  <  1:79739/247‑1 (MQ=255)                                                                                                                                                                      cAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAACGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAAc                                                                                                                                                                             >  1:132014/1‑123 (MQ=255)                                                                                                                                                                                                                 ccATTGTCATCACGTCCTTTATAAAGCGGGGCGAGGGAACATCCGGCAAACAGGGTCGCCATGCCGATAAACGCGGCAACCCACAACCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAt  <  2:77968/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        TGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGAT  >  NC_000913/7384‑7842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑