Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,133	T→G	S146S (TCT→TCG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,133	0	T	G	100.0%	95.5 / NA	34	S146S (TCT→TCG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/11);  total (23/11)

GCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCC  >  NC_000913/4636899‑4637370                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              gcAGGCGTTTTATCAGCTACAACATCGCCCGTTTAGCGCCAATATCGGTGGCATTAACAGAGTACGCAATGAATATCATGTCTATATGACCGATTAGCAGGGCAAAGTCTTGTTCGATTAGGCAAATAACGACGATGGGCAGGATTATTCGCGCTGGTATGACGTCTGGCTACCGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGATCGGTGAGATATGTTGCCg                                                                                                                                                                                                                               >  2:145956/1‑251 (MQ=255)     gCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAATGAATATCAGGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGTGCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGcgcc                                                                                                                                                                                                                           <  1:29484/251‑4 (MQ=255)            aTCAGCTGCAACATCGCCCGTTTCGCGCAATTATCGGTGGCATTAACAAAGAGCGCAATGTATATCATGTATATCTGACCGATTCGCAGGGTAACGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGAGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTACCGCGACGATTATg                                                                                                                                                                                                                    >  2:25510/1‑251 (MQ=255)                 cTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGACTATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAAAGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACgg                                                                                                                                                                                                               >  2:214686/1‑251 (MQ=255)                                 ttCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTgg                                                                                                                                                                                                <  1:71069/250‑1 (MQ=255)                                              cGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGt                                                                                                                                                                                    >  2:254356/1‑249 (MQ=255)                                                          cAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAAcg                                                                                                                                                                      <  1:49835/251‑1 (MQ=255)                                                           aaaGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGAGATGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGCTGTATGTTGCCGCGACGATTATGGACGTCTCGCGGCTTCTTGGCGTTTTGAGCGTAGGCAAACAGAAcgc                                                                                                                                                                     >  2:140776/1‑251 (MQ=255)                                                                   cAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTAGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGAGCAGCACGTTGAAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATgg                                                                                                                                                             >  1:120553/1‑251 (MQ=255)                                                                         atatCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCgg                                                                                                                                                       >  1:98519/1‑251 (MQ=255)                                                                                  cTATATGACCGATTCGCAGGGTAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGGTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGc                                                                                                                                              >  1:2193/1‑251 (MQ=255)                                                                                        gACCGATTCGCAGGGTAAAGTATGGTGCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTGCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTagc                                                                                                                                         <  2:207078/250‑1 (MQ=255)                                                                                                       taaaGAATTGTTCGATTCGGCAAATCAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTAGCTAACGTTGCGTGGTCAGTATGGTGCGCGAAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGCTGTCTGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTAt                                                                                                                         >  2:264671/2‑251 (MQ=255)                                                                                                        aaaGTATTGTTCGATTCGGCAAATAAAGCCGCTGGGCAGGAATATACGCGCTGGAATGACGTCTGGTTAAAATTGCGTGATCAGTCAGGTGCGCGCAGCACGTTGCAAAATCCTGACGATCACGAAAGTACGGAGATGTATGATGACGCGAAGATTATGGACGGCTCGCGGCTTATTAGCGTTTTGAGCGTAGGCAAACCGCACGAGGCGATGGCTCCGGTCATTCAACGAAGCGAGCGGCGAATTTTATg                                                                                                                        >  1:238418/1‑251 (MQ=255)                                                                                                                   cgcttCGGCAAATAAAGCCGCTGGGCAGGATTATTCGCGCTGGAAGGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCACGCCGATTATGGACGGCGCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAACGGCGGATTTTATGGGCCAGCGcc                                                                                                              >  2:155974/4‑250 (MQ=255)                                                                                                                     aTTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCAtt                                                                                                           >  1:160831/1‑251 (MQ=255)                                                                                                                             aaTAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTggg                                                                                                   >  2:125039/1‑251 (MQ=255)                                                                                                                                  aGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCAtttt                                                                                                         >  1:17313/1‑240 (MQ=255)                                                                                                                                  aGCCGTTGGGCAGGATGATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCAtttt                                                                                                         <  2:17313/240‑1 (MQ=255)                                                                                                                                   gCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGc                                                                                             >  1:219291/1‑251 (MQ=255)                                                                                                                                    ccGTTGGGCAGGATTATTCGCGCTGGAATGCCGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGc                                                                                             >  1:82470/1‑250 (MQ=255)                                                                                                                                                attattCGCGCTGGAATGACGTCTGGCTAACGTTGCTGGGTCAGTATGGGGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTTCTGGTGATTGCACTGGTGATTGgc                                                                                <  2:194477/251‑1 (MQ=255)                                                                                                                                                   attCGAGCTGGAATGACGTCTGACTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGCCATTAAGCGTAGCGAGCGGGGAATTTTATGGGCCCGCGCCATTTTGCTGGGGATTGCACTGGTGGTTggggcg                                                                             >  2:174931/1‑247 (MQ=255)                                                                                                                                                             gAATGACGTATGGCTAACGTTGAGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGttt                                                                   >  1:210598/1‑251 (MQ=255)                                                                                                                                                                    gTCTGGCTAACGTTGCGTGGGCAGTATGGTGCGCGCAGCACGTTGCAAAATACTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACAGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGAt                                                            >  1:173972/1‑251 (MQ=255)                                                                                                                                                                     gcTGGCTAACTTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACTCTGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATGTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATc                                                           <  1:15057/250‑1 (MQ=255)                                                                                                                                                                           tAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCATAATCCTGCCGATCCCGACAGTTCGGCGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGCTTGCACTGGTGCTTGGCGCAGGCATGGTTTGGTGGATCAACCGc                                                     >  2:120640/1‑251 (MQ=255)                                                                                                                                                                            aaCGTTGCGTGGTAAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAAAGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTAATGGGCCAGCGACATTTTGCTGGGGATTGCACTGGGGATTGGCGCAGGAATGGTTTGGTGGATCAACCGct                                                    >  2:198444/1‑251 (MQ=255)                                                                                                                                                                              cGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGctcc                                                  >  1:208605/1‑250 (MQ=255)                                                                                                                                                                                    gTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCCACCGCTCCATTGcc                                            >  1:163228/1‑251 (MQ=255)                                                                                                                                                                                                gTGCGCTCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGc                                <  1:227799/251‑1 (MQ=255)                                                                                                                                                                                                     ggcagcaCTTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATGTTGCGGGGGATTGCACGGTTGATTGGCGCAGGCATGGTTTGGTTGATCAACCTCTGCATTGCCCGGCTCATTCGCTATGc                           <  2:164339/250‑1 (MQ=255)                                                                                                                                                                                                             gTTGCAAAATCCTGCCGATCCCGAAAGTTCGGTGATGTATGTTGCCGCGCCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCTCACTCGCTATGCCGATTCCg                   <  1:28596/251‑1 (MQ=255)                                                                                                                                                                                                                              aTCCGGAAAGTTCGGTGATGGATGTTGCCGCGCCGATTATGGACGGCTCTCGGCTTATTGGCGTTTGGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGCTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCCATTGCCCGGCGCACTCGCTATGCCGATCCCGTCACTGACAATAAGccc  <  2:136695/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              GCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCGGCTTATTGGCGTTTTGAGCGTAGGCAAACCGAACGCGGCGATGGCTCCGGTCATTAAGCGTAGCGAGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCC  >  NC_000913/4636899‑4637370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑