Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,278	C→T	N314N (AAC→AAT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,278	0	C	T	100.0%	70.1 / NA	26	N314N (AAC→AAT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/11);  total (15/11)

ATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGG  >  NC_000913/1034‑1477                                                                                                                                                                                                                                                     |                                                                                                                                                                                                        ataCCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGc                                                                                                                                                                                                    >  2:68385/1‑250 (MQ=255)       gCGACCCGCGTCAGGGGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAAGAATATGGCAATGTTc                                                                                                                                                                                             >  2:79513/1‑251 (MQ=255)                  cAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGATCTTGCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCGCATTGGTGCCAGCCGTGATGAAGGCGAATTGCCGGTCAAGGGGATTTCCAATCGGAATAATATTGCAATGTGCAGCGGTTccgg                                                                                                                                                                                  <  2:257686/251‑4 (MQ=255)                                     tgttgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGAt                                                                                                                                                                <  1:202399/250‑1 (MQ=255)                                           aGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTAAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGc                                                                                                                                                         >  1:173914/1‑251 (MQ=255)                                                  gTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATggcgg                                                                                                                                                  <  1:186235/251‑1 (MQ=255)                                                                 gATGGAGTGTTCCTACTTCGGCGATAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCATCAAGCTCCCGGCACGCTAATTGGTGCAAGCTGTGATGAAGACGAATTACCGGTAAAGAGCATTTCCAATCTGAATAATATTGCAATATTCAGTGTTTCCGGCCCGTGGATGAAAGGGTTAGTTGGCATGGTGGtgcgcgggtttga                                                                                                                                     >  2:194185/1‑242 (MQ=255)                                                                 gATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGc                                                                                                                                     >  1:128877/1‑249 (MQ=255)                                                                     gctcTTTCCTACTGCGGCGCTAATGTTCTTCACCCCCCCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAACAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGTATTTCCGGTCAAGGGCATTTCCAATCTGAAGAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATTAAATCGATGGTTGGCATGGCGGCGCGCGTGTTGGCAGCGAt                                                                                                                               <  2:160064/248‑1 (MQ=255)                                                                                                  tcACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATTCCTGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCTGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGTATTTCCGTGGTGCTg                                                                                                   <  1:236889/250‑1 (MQ=255)                                                                                                       cccGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACg                                                                                             >  1:85857/1‑251 (MQ=255)                                                                                                           cACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAat                                                                                         >  1:142823/1‑251 (MQ=255)                                                                                                                 tACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCtt                                                                                   >  1:89263/1‑251 (MQ=255)                                                                                                                        aTCGCCCAGGTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATa                                                                            <  1:237735/251‑1 (MQ=255)                                                                                                                                     aGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTCATGAAGACGCATTACCGGTCAAGGGAATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTCTTTCCGTGGAGCTGGTTACGCCATCATCTTCCGCATACAGTATCCGTTTc                                                               >  1:254309/1‑251 (MQ=255)                                                                                                                                       aTCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTGCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGTCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTAGCAGTTTCTg                                                             <  2:135321/251‑1 (MQ=255)                                                                                                                                        tCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGACTAAAGTATCAGTTTCTGc                                                            >  1:52144/1‑251 (MQ=255)                                                                                                                                              gCCTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTGTCTGCGTTccg                                                      <  2:20979/251‑2 (MQ=255)                                                                                                                                                cTGATTAAAAATACCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATTCAGTATCAGTTTCTGCGTTccgc                                                     >  1:175886/1‑248 (MQ=255)                                                                                                                                                            aCCGGAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACtgtgt                                        >  2:203279/1‑251 (MQ=255)                                                                                                                                                                gAAATCCTCAAGCTCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGa                                    <  2:142823/251‑1 (MQ=255)                                                                                                                                                                              ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa                       <  1:125756/250‑1 (MQ=255)                                                                                                                                                                                            gggTGCCAGCCTGGATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGTTTGGCATGGCGGCGCGCGTGTTTGCAGCTATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTGCCGAAGACTGTATCAGTTTCTGCGTGCCTCAAAGCGACTGTGTGCGAGCTGAGCGGGCAATGCAGGAAGAGTTc         <  2:250914/249‑1 (MQ=255)                                                                                                                                                                                             ggTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCtc       >  1:27863/1‑250 (MQ=255)                                                                                                                                                                                               tGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGCGCTGAACGGGCAATGCAGGAAGAGTTCTAcc     >  2:103020/1‑251 (MQ=255)                                                                                                                                                                                                   acccGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCGTGGTGGTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCCAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg  >  2:187916/3‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                        ATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGG  >  NC_000913/1034‑1477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑